Abstract：
:Migrating fibroblasts undergo contact inhibition of locomotion (CIL), a process that was discovered five decades ago and still is not fully understood at the molecular level. We identify the Slit2-Robo4-srGAP2 signaling network as a key regulator of CIL in fibroblasts. CIL involves highly dynamic contact protrusions with a specialized actin cytoskeleton that stochastically explore cell-cell overlaps between colliding fibroblasts. A membrane curvature-sensing F-BAR domain pre-localizes srGAP2 to protruding edges and terminates their extension phase in response to cell collision. A FRET-based biosensor reveals that Rac1 activity is focused in a band at the tip of contact protrusions, in contrast to the broad activation gradient in contact-free protrusions. SrGAP2 specifically controls the duration of Rac1 activity in contact protrusions, but not in contact-free protrusions. We propose that srGAP2 integrates cell edge curvature and Slit-Robo-mediated repulsive cues to fine-tune Rac1 activation dynamics in contact protrusions to spatiotemporally coordinate CIL.
journal_name
Dev Celljournal_title
Developmental cellauthors
Fritz RD,Menshykau D,Martin K,Reimann A,Pontelli V,Pertz Odoi
10.1016/j.devcel.2015.09.002subject
Has Abstractpub_date
2015-10-12 00:00:00pages
78-92issue
1eissn
1534-5807issn
1878-1551pii
S1534-5807(15)00582-1journal_volume
35pub_type
杂志文章abstract：:Airway smooth muscle is best known for its role as an airway constrictor in diseases such as asthma. However, its function in lung development is debated. A prevalent model, supported by in vitro data, posits that airway smooth muscle promotes lung branching through peristalsis and pushing intraluminal fluid to branch...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2020.02.001
更新日期：2020-04-06 00:00:00
abstract：:Cytokinins affect plant immunity to various pathogens; however, the mechanisms coupling plant-derived cytokinins to pathogen responses have been elusive. Here, we found that plant-derived cytokinins promote resistance of Arabidopsis to Pseudomonas syringae pv. tomato DC3000 (Pst). Modulated cytokinin levels or signali...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2010.07.011
更新日期：2010-08-17 00:00:00
abstract：:Embryonic organizers establish gradients of diffusible signaling molecules to pattern the surrounding cells. Here, we elucidate an additional mechanism of embryonic organizers that is a secondary consequence of morphogen signaling. Using pharmacological and localized transgenic perturbations, 4D imaging of the zebrafi...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2019.05.014
更新日期：2019-06-17 00:00:00
abstract：:The Hippo (Hpo) signaling pathway controls tissue growth and organ size in species ranging from Drosophila to mammals and is deregulated in a wide range of human cancers. The core pathway consists of the Hpo/Warts (Wts) kinase cassette that phosphorylates and inactivates the transcriptional coactivator Yorkie (Yki). H...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2011.12.011
更新日期：2012-02-14 00:00:00
abstract：:In a recent issue of Cell, Coureuil et al. (2010) show that type IV pili of Neisseria meningitidis-the causative bacterium of cerebrospinal meningitis-bind to the endothelial β2-adrenoceptor and elicit biased β-arrestin signaling resulting in microvilli formation and paracytotic bacterial migration. ...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2010.12.011
更新日期：2011-01-18 00:00:00
abstract：:piRNAs are known to silence transposable elements, but not all piRNAs match transposon sequences. Recent studies from Shen et al. (2018) and Zhang et al. (2018) identify rules for piRNA target recognition in Caenorhabditis elegans. Permissive pairing rules allow targeting of essentially all germline mRNAs, while prote...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2018.03.006
更新日期：2018-03-26 00:00:00
abstract：:Exponential increase of cell numbers in early embryos requires large amounts of DNA precursors (deoxyribonucleoside triphosphates (dNTPs)). Little is understood about how embryos satisfy this demand. We examined dNTP metabolism in the early Drosophila embryo, in which gastrulation is preceded by 13 sequential nuclear ...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2017.06.013
更新日期：2017-08-07 00:00:00
abstract：:The role of mitochondria in Drosophila programmed cell death remains unclear, although certain gene products that regulate cell death seem to be evolutionarily conserved. We find that developmental programmed cell death stimuli in vivo and multiple apoptotic stimuli ex vivo induce dramatic mitochondrial fragmentation ...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2007.02.002
更新日期：2007-05-01 00:00:00
abstract：:22q11 deletion (del22q11) syndrome is characterized genetically by heterozygous deletions within chromosome 22q11 and clinically by a constellation of congenital malformations of the aortic arch, heart, thymus, and parathyroid glands described as DiGeorge syndrome (DGS). Here, we report that compound heterozygosity of...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2005.12.002
更新日期：2006-01-01 00:00:00
abstract：:The generation and shedding of extracellular vesicles (EVs), including exosomes and microvesicles (MVs), by cells has emerged as a form of intercellular communication with important roles in several physiological processes and diseases such as cancer. These membrane-enclosed packets can transfer specific proteins, RNA...
journal_title：Developmental cell
pub_type： 杂志文章,评审
doi：10.1016/j.devcel.2016.04.019
更新日期：2016-05-23 00:00:00
abstract：:Neutrophils are essential to the homeostatic mission of safeguarding host tissues, responding rapidly and diversely to breaches of the host's barriers to infection, and returning tissues to a sterile state. In response to specific stimuli, neutrophils extrude modified chromatin structures decorated with specific cytop...
journal_title：Developmental cell
pub_type： 杂志文章,评审
doi：10.1016/j.devcel.2018.01.019
更新日期：2018-03-12 00:00:00
abstract：:The kinase Plk1 plays multiple roles in regulating mitotic progression, including stabilization of spindle poles, but its substrates are largely unknown. A new study by Yamamoto and coworkers has identified a centrosomal protein, Kizuna (Kiz), as a mitotic substrate of Plk1 (Oshimori et al., 2006). Phosphorylation of ...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2006.09.012
更新日期：2006-10-01 00:00:00
abstract：:Mammalian lungs are comprised of conducting airways and alveoli. How the distinct epithelial linings of these two zones are differentially specified and maintained is not fully understood. In this issue of Developmental Cell, two groups find critical roles for the Hippo pathway in regulation of lung progenitor cell di...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2014.07.002
更新日期：2014-07-28 00:00:00
abstract：:Piwi family protein Aubergine (Aub) maintains genome integrity in late germ cells of the Drosophila ovary through Piwi-associated RNA-mediated repression of transposon activities. Although it is highly expressed in germline stem cells (GSCs) and early progeny, it remains unclear whether it plays any roles in early GSC...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2017.03.023
更新日期：2017-04-24 00:00:00
abstract：:Dynamin family members are large GTPases that assemble into multimeric spirals. These spirals promote membrane fission or fusion, or they inhibit processes such as viral replication. Two new studies by Chappie et al. and Gao et al. in a recent issue of Nature identify interactions between subunits of dynamin spirals, ...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2010.05.001
更新日期：2010-05-18 00:00:00
abstract：:Changes to cell shape and interaction drive animal tissue morphogenesis. Reporting now in Developmental Cell, Del Signore et al. (2018) describe active lengthening of cell-cell contacts by Arp2/3-based actin networks. Through cycles of contact lengthening and shortening, the developing Drosophila eye passes through va...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2018.02.003
更新日期：2018-02-26 00:00:00
abstract：:The TGF-beta family member Nodal is one of the most potent molecules known to an embryo. Studies on Nodal regulation thus far have focused on its transcription, maturation, and interaction with antagonists. Two recent studies on the role of microRNAs (miRNAs) in Nodal signaling now reveal that something important was ...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2007.09.012
更新日期：2007-10-01 00:00:00
abstract：:Novel body structures are often generated by the redeployment of ancestral components of the genome. In this issue of Developmental Cell, Glassford et al. (2015) present a thorough analysis of the co-option of a gene regulatory network in the origin of an evolutionary novelty. ...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2015.08.022
更新日期：2015-09-14 00:00:00
abstract：:Hox proteins gain specificity by interacting with TALE-class cofactors. In a recent issue of Cell and in this issue of Developmental Cell, Crocker et al. (2015) and Amin et al. (2015), respectively, demonstrate that non-canonical Hox/TALE binding sequences play a major role in the regionalized regulation of target gen...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2015.01.030
更新日期：2015-02-09 00:00:00
abstract：:Steroid hormones have long been thought to enter target cells via passive diffusion through the plasma membrane. Now, reporting in Developmental Cell, Okamoto et al. (2018) demonstrate that, at least for Drosophila, steroid hormones require a protein transporter for cellular entry. ...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2018.10.022
更新日期：2018-11-05 00:00:00
abstract：:We have used Drosophila ovarian follicle stem cells (FSCs) to study how stem cells are regulated by external signals and draw three main conclusions. First, the spatial definition of supportive niche positions for FSCs depends on gradients of Hh and JAK-STAT pathway ligands, which emanate from opposite, distant sites....
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2012.09.010
更新日期：2012-10-16 00:00:00
abstract：:Blood vessels form an important interface between the environment and the organism by carrying oxygen and nutrients to all cells and thus determining cellular metabolism. It is therefore not surprising that oxygen and metabolism influence the development of the vascular network. Here, we discuss recent insights regard...
journal_title：Developmental cell
pub_type： 杂志文章,评审
doi：10.1016/j.devcel.2009.01.003
更新日期：2009-02-01 00:00:00
abstract：:Theoretical modeling is central to elucidating underlying principles of emergent properties of complex systems. In cell and developmental biology, the last 15 years have witnessed a convergence of empirical and modeling approaches for fresh perspectives. The role of cell division in coordinating size, shape, and fate ...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2016.09.008
更新日期：2016-09-26 00:00:00
abstract：:CLIPs are microtubule plus end-associated proteins that mediate interactions required for cell polarity and cell division. Here we demonstrate that budding yeast Bik1, unlike its human ortholog CLIP-170, is targeted to the microtubule plus end by a kinesin-dependent transport mechanism. Bik1 forms a complex with the k...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2004.05.001
更新日期：2004-06-01 00:00:00
abstract：:Myosin phosphatase-targeting subunit 1 (MYPT1) binds to the catalytic subunit of protein phosphatase 1 (PP1C). This binding is believed to target PP1C to specific substrates including myosin II, thus controlling cellular contractility. Surprisingly, we found that during mitosis, mammalian MYPT1 binds to polo-like kina...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2008.02.013
更新日期：2008-05-01 00:00:00
abstract：:Cell stress often triggers inflammation as a protective response to tissue damage or infection. In this issue of Developmental Cell, Sullivan et al. (2020) reveal an unexpected role for TRAIL receptors in ER-stress-induced inflammation. ...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2020.03.004
更新日期：2020-03-23 00:00:00
abstract：:Boundary formation and epithelialization are crucial processes in the morphological segmentation of vertebrate somites. By a genetic screening procedure with zebrafish, we identified two genes, integrinalpha5 (itga5) and fibronectin (fn), required for these processes. Fibronectin proteins accumulate at somite boundari...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2005.03.006
更新日期：2005-04-01 00:00:00
abstract：:In this issue of Developmental Cell, Isabella and Horne-Badovinac (2016) show that Rab10 directs site-specific secretion of basement membrane components, which assemble into fibrils that spool out to elongate the Drosophila egg chamber. These findings establish the basement membrane's active role in tissue sculpting. ...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2016.06.028
更新日期：2016-07-11 00:00:00
abstract：:Ribosomes perform protein synthesis but are also involved in signaling processes, the full extent of which are still being uncovered. We report that phenotypes of mutating ribosomal proteins (Rps) are largely due to signaling. Using Drosophila, we discovered that a bZip-domain protein, Xrp1, becomes elevated in Rp mut...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2018.07.003
更新日期：2018-08-20 00:00:00
abstract：:Eukaryotic genomes are organized within the nucleus through interactions with inner nuclear membrane (INM) proteins. How chromatin tethering to the INM is controlled in interphase and how this process contributes to subsequent mitotic nuclear envelope (NE) remodeling remains unclear. We have probed these fundamental q...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2020.01.028
更新日期：2020-04-06 00:00:00
