Abstract：
:Although it is well recognized that haematopoietic stem cells (HSCs) develop from a specialized population of endothelial cells known as haemogenic endothelium, the regulatory pathways that control this transition are not well defined. Here we identify Sox17 as a key regulator of haemogenic endothelial development. Analysis of Sox17-GFP reporter mice revealed that Sox17 is expressed in haemogenic endothelium and emerging HSCs and that it is required for HSC development. Using the mouse embryonic stem cell differentiation model, we show that Sox17 is also expressed in haemogenic endothelium generated in vitro and that it plays a pivotal role in the development and/or expansion of haemogenic endothelium through the Notch signalling pathway. Taken together, these findings position Sox17 as a key regulator of haemogenic endothelial and haematopoietic development.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Clarke RL,Yzaguirre AD,Yashiro-Ohtani Y,Bondue A,Blanpain C,Pear WS,Speck NA,Keller Gdoi
10.1038/ncb2724subject
Has Abstractpub_date
2013-05-01 00:00:00pages
502-10issue
5eissn
1465-7392issn
1476-4679pii
ncb2724journal_volume
15pub_type
杂志文章abstract：:The tumour-suppressor gene ATM, mutations in which cause the human genetic disease ataxia telangiectasia (A-T), encodes a key protein kinase that controls the cellular response to DNA double-strand breaks (DSBs). DNA DSBs caused by ionizing radiation or chemicals result in rapid ATM autophosphorylation, leading to che...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1651
更新日期：2007-11-01 00:00:00
abstract：:The presence of disseminated tumour cells (DTCs) in bone marrow is predictive of poor metastasis-free survival of patients with breast cancer with localized disease. DTCs persist in distant tissues despite systemic administration of adjuvant chemotherapy. Many assume that this is because the majority of DTCs are quies...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-018-0267-0
更新日期：2019-02-01 00:00:00
abstract：:Obesity occurs when excess energy accumulates in white adipose tissue (WAT), whereas brown adipose tissue (BAT), specialized for energy expenditure through thermogenesis, potently counteracts obesity. Factors that induce brown adipocyte commitment and energy expenditure would be a promising defence against adiposity. ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2867
更新日期：2013-12-01 00:00:00
abstract：:Collective cell migration occurs in a range of contexts: cancer cells frequently invade in cohorts while retaining cell-cell junctions. Here we show that collective invasion by cancer cells depends on decreasing actomyosin contractility at sites of cell-cell contact. When actomyosin is not downregulated at cell-cell c...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2133
更新日期：2011-01-01 00:00:00
abstract：:Genomic integrity is maintained by checkpoints that guard against undesired replication after DNA damage. Here, we show that CDT1, a licensing factor of the pre-replication complex (preRC), is rapidly proteolysed after UV- or gamma-irradiation. The preRC assembles on replication origins at the end of mitosis and durin...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1061
更新日期：2003-11-01 00:00:00
abstract：:Membrane association with mother centriole (M-centriole) distal appendages is critical for ciliogenesis initiation. How the Rab GTPase Rab11-Rab8 cascade functions in early ciliary membrane assembly is unknown. Here, we show that the membrane shaping proteins EHD1 and EHD3, in association with the Rab11-Rab8 cascade, ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3109
更新日期：2015-03-01 00:00:00
abstract：:Metastasis is an inefficient process, especially during colonization at a distant organ. This bottleneck underlies the importance of the metastatic niche for seeding and outgrowth of metastases. Here, we classify the common functions of different metastatic niches: anchorage, survival support, protection from external...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/s41556-018-0145-9
更新日期：2018-08-01 00:00:00
abstract：:Different sites of plasma membrane attachment may underlie functional differences between isoforms of Ras. Here we show that palmitoylation and farnesylation targets H-ras to lipid rafts and caveolae, but that the interaction of H-ras with these membrane subdomains is dynamic. GTP-loading redistributes H-ras from raft...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35070050
更新日期：2001-04-01 00:00:00
abstract：:Extracellular signal-regulated kinase (ERK) controls fundamental cellular functions, including cell fate decisions. In PC12, cells shifting ERK activation from transient to sustained induces neuronal differentiation. As ERK associates with both regulators and effectors, we hypothesized that the mechanisms underlying t...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1994
更新日期：2009-12-01 00:00:00
abstract：:Unrepaired DNA double-strand breaks (DSBs) cause genetic instability that leads to malignant transformation or cell death. Cells respond to DSBs with the ordered recruitment of signalling and repair proteins to the site of lesion. Protein modification with ubiquitin is crucial for the signalling cascade, but how ubiqu...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2367
更新日期：2011-10-23 00:00:00
abstract：:Epithelial-mesenchymal transition (EMT) is a crucial embryonic programme that is executed by various EMT transcription factors (EMT-TFs) and is aberrantly activated in cancer and other diseases. However, the causal role of EMT and EMT-TFs in different disease processes, especially cancer and metastasis, continues to b...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/s41556-018-0196-y
更新日期：2019-01-01 00:00:00
abstract：:Clathrin-coated vesicles mediate endocytosis and transport between the trans-Golgi network (TGN) and endosomes in eukaryotic cells. Clathrin adaptors play central roles in coat assembly, interacting with clathrin, cargo and membranes. Two main types of clathrin adaptor act in TGN-endosome traffic: GGA proteins and the...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2427
更新日期：2012-02-19 00:00:00
abstract：:Altered DNA methylation and associated destabilization of genome integrity and function is a hallmark of cancer. Replicative senescence is a tumour suppressor process that imposes a limit on the proliferative potential of normal cells that all cancer cells must bypass. Here we show by whole-genome single-nucleotide bi...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2879
更新日期：2013-12-01 00:00:00
abstract：:Formins have been implicated in the regulation of cytoskeletal structure in animals and fungi. Here we show that the formins Bni1 and Bnr1 of budding yeast stimulate the assembly of actin filaments that function as precursors to tropomyosin-stabilized cables that direct polarized cell growth. With loss of formin funct...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb718
更新日期：2002-01-01 00:00:00
abstract：:LATS (large tumour suppressor) is a family of conserved tumour suppressors identified in Drosophila and mammals. Here we show that human LATS1 binds to LIMK1 in vitro and in vivo and colocalizes with LIMK1 at the actomyosin contractile ring during cytokinesis. LATS1 inhibits both the phosphorylation of cofilin by LIMK...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1140
更新日期：2004-07-01 00:00:00
abstract：:Autophagy is a lysosome-based degradation pathway. During autophagy, lysosomes fuse with autophagosomes to form autolysosomes. Following starvation-induced autophagy, nascent lysosomes are formed from autolysosomal membranes through an evolutionarily conserved cellular process, autophagic lysosome reformation (ALR), w...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2557
更新日期：2012-09-01 00:00:00
abstract：:Gastrulation leads to three germ layers--ectoderm, mesoderm and endoderm--that are separated by two basement membranes. In the mouse embryo, the emergent gut endoderm results from the widespread intercalation of cells of two distinct origins: pluripotent epiblast-derived definitive endoderm (DE) and extra-embryonic vi...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3070
更新日期：2014-12-01 00:00:00
abstract：:Collective cell migration is essential for both physiological and pathological processes. Adherens junctions (AJs) maintain the integrity of the migrating cell group and promote cell coordination while allowing cellular rearrangements. Here, we show that AJs undergo a continuous treadmilling along the lateral sides of...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2985
更新日期：2014-07-01 00:00:00
abstract：:Bcl-2 stimulates mutagenesis after the exposure of cells to DNA-damaging agents. However, the biological mechanisms of Bcl-2-mediated mutagenesis have remained largely obscure. Here we demonstrate that the Bcl-2-mediated suppression of hMSH2 expression results in a reduced cellular capacity to repair mismatches. The p...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1215
更新日期：2005-02-01 00:00:00
abstract：:The molecular requirements and morphology of migrating cells can vary depending on matrix geometry; therefore, predicting the optimal migration strategy or the effect of experimental perturbation is difficult. We present a model of cell motility that encompasses actin-polymerization-based protrusions, actomyosin contr...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2775
更新日期：2013-07-01 00:00:00
abstract：:Phosphoinositides (PtdIns) control fundamental cell processes, and inherited defects of PtdIns kinases or phosphatases cause severe human diseases, including Lowe syndrome due to mutations in OCRL, which encodes a PtdIns(4,5)P2 5-phosphatase. Here we unveil a lysosomal response to the arrival of autophagosomal cargo i...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3386
更新日期：2016-08-01 00:00:00
abstract：:Work over the past two years has led to a breakthrough in our understanding of the molecular basis of the directionality of the kinesin motor proteins. This breakthrough has come first from the reversal of directionality of the kinesin-related motor Ncd, followed closely by the reversal of kinesin's directionality and...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/14113
更新日期：1999-10-01 00:00:00
abstract：:Fetal haematopoietic stem cells (HSCs) self-renew extensively to build the blood system from scratch. The protein Lin28b negatively regulates the microRNA let-7 to keep levels of its target Hmga2 high, hence conferring high self-renewal potential to fetal HSCs. This regulatory circuit can be experimentally modulated t...
journal_title：Nature cell biology
pub_type： 评论,新闻
doi：10.1038/ncb2817
更新日期：2013-08-01 00:00:00
abstract：:Telomere attrition and other forms of telomere damage can activate the ATM kinase pathway. What generates the DNA damage signal at mammalian chromosome ends or at other double-strand breaks is not known. Telomere dysfunction is often accompanied by disappearance of the 3' telomeric overhang, raising the possibility th...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1275
更新日期：2005-07-01 00:00:00
abstract：:Given the important role of the hypothalamus in regulating feeding and metabolism, there has been considerable interest in a possible function for hypothalamic stem cells in modulating body weight in health and disease. Mice given a high-fat diet develop inflammation in the hypothalamus and lose key types of neurons. ...
journal_title：Nature cell biology
pub_type： 新闻
doi：10.1038/ncb2599
更新日期：2012-10-01 00:00:00
abstract：:The Ndc80 complex (Ndc80, Nuf2, Spc24 and Spc25) is a highly conserved kinetochore protein essential for end-on anchorage to spindle microtubule plus ends and for force generation coupled to plus-end polymerization and depolymerization. Spc24/Spc25 at one end of the Ndc80 complex binds the kinetochore. The N-terminal ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3323
更新日期：2016-04-01 00:00:00
abstract：:It has long-been accepted that normal somatic cells have intrinsic mechanisms that limit their proliferative lifespan. Recent work has now challenged this view by demonstrating that extrinsic factors might be determining proliferative potential. ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb0202-e25
更新日期：2002-02-01 00:00:00
abstract：:Cancer-induced immune responses affect tumour progression and therapeutic response. In multiple murine models and clinical datasets, we identified large variations of neutrophils and macrophages that define 'immune subtypes' of triple-negative breast cancer (TNBC), including neutrophil-enriched (NES) and macrophage-en...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-019-0373-7
更新日期：2019-09-01 00:00:00
abstract：:Inactivation of the von Hippel-Lindau (VHL) tumour-suppressor protein (pVHL) is associated with the von Hippel-Lindau cancer syndrome and the majority of kidney cancers. New evidence suggests that pVHL has properties of an F-box protein that targets the alpha-subunits of hypoxia-inducible factor (HIF)-1 for oxygen-dep...
journal_title：Nature cell biology
pub_type： 评论,新闻
doi：10.1038/35017129
更新日期：2000-07-01 00:00:00
abstract：:YAP (Yes-associated protein) is a transcription co-activator in the Hippo tumour suppressor pathway and controls cell growth, tissue homeostasis and organ size. YAP is inhibited by the kinase Lats, which phosphorylates YAP to induce its cytoplasmic localization and proteasomal degradation. YAP induces gene expression ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3111
更新日期：2015-04-01 00:00:00
