Abstract:
:The Ndc80 complex (Ndc80, Nuf2, Spc24 and Spc25) is a highly conserved kinetochore protein essential for end-on anchorage to spindle microtubule plus ends and for force generation coupled to plus-end polymerization and depolymerization. Spc24/Spc25 at one end of the Ndc80 complex binds the kinetochore. The N-terminal tail and CH domains of Ndc80 bind microtubules, and an internal domain binds microtubule-associated proteins (MAPs) such as the Dam1 complex. To determine how the microtubule- and MAP-binding domains of Ndc80 contribute to force production at the kinetochore in budding yeast, we have inserted a FRET tension sensor into the Ndc80 protein about halfway between its microtubule-binding and internal loop domains. The data support a mechanical model of force generation at metaphase where the position of the kinetochore relative to the microtubule plus end reflects the relative strengths of microtubule depolymerization, centromere stretch and microtubule-binding interactions with the Ndc80 and Dam1 complexes.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Suzuki A,Badger BL,Haase J,Ohashi T,Erickson HP,Salmon ED,Bloom Kdoi
10.1038/ncb3323subject
Has Abstractpub_date
2016-04-01 00:00:00pages
382-92issue
4eissn
1465-7392issn
1476-4679pii
ncb3323journal_volume
18pub_type
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