Abstract：
:Clathrin-coated vesicles mediate endocytosis and transport between the trans-Golgi network (TGN) and endosomes in eukaryotic cells. Clathrin adaptors play central roles in coat assembly, interacting with clathrin, cargo and membranes. Two main types of clathrin adaptor act in TGN-endosome traffic: GGA proteins and the AP-1 complex. Here we characterize the relationship between GGA proteins, AP-1 and other TGN clathrin adaptors using live-cell and super-resolution microscopy in yeast. We present evidence that GGA proteins and AP-1 are recruited sequentially in two waves of coat assembly at the TGN. Mutations that decrease phosphatidylinositol 4-phosphate (PtdIns(4)P) levels at the TGN slow or uncouple AP-1 coat assembly from GGA coat assembly. Conversely, enhanced PtdIns(4)P synthesis shortens the time between adaptor waves. Gga2p binds directly to the TGN PtdIns(4)-kinase Pik1p and contributes to Pik1p recruitment. These results identify a PtdIns(4)P-based mechanism for regulating progressive assembly of adaptor-specific clathrin coats at the TGN.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Daboussi L,Costaguta G,Payne GSdoi
10.1038/ncb2427subject
Has Abstractpub_date
2012-02-19 00:00:00pages
239-48issue
3eissn
1465-7392issn
1476-4679pii
ncb2427journal_volume
14pub_type
杂志文章abstract：:Coordination of microtubules and the actin cytoskeleton is important in several types of cell movement. mDia1 is a member of the formin-homology family of proteins and an effector of the small GTPase Rho. It contains the Rho-binding domain in its amino terminus and two distinct regions of formin homology, FH1 in the m...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35050598
更新日期：2001-01-01 00:00:00
abstract：:Centromeres are specialized chromosomal domains that direct kinetochore assembly during mitosis. CENP-A (centromere protein A), a histone H3-variant present exclusively in centromeric nucleosomes, is thought to function as an epigenetic mark that specifies centromere identity. Here we identify the essential centromere...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1899
更新日期：2009-07-01 00:00:00
abstract：:KLF4 (GKLF/EZF) encodes a transcription factor that is associated with both tumour suppression and oncogenesis. We describe the identification of KLF4 in a functional genomic screen for genes that bypass RAS(V12)-induced senescence. However, in untransformed cells, KLF4 acts as a potent inhibitor of proliferation. KLF...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1314
更新日期：2005-11-01 00:00:00
abstract：:For accurate segregation of chromosomes during cell division, microtubule fibres must attach sister kinetochores to opposite poles of the mitotic spindle (bi-orientation). Aurora kinases are linked to oncogenesis and have been implicated in the regulation of chromosome-microtubule attachments. Although loss of Aurora ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1102
更新日期：2004-03-01 00:00:00
abstract：:The cyclical protrusion and retraction of the leading edge is a hallmark of many migrating cells involved in processes such as development, inflammation and tumorigenesis. The molecular identity of the signalling mechanisms that control these cycles has remained unknown. Here, we used live-cell imaging of biosensors t...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2231
更新日期：2011-06-01 00:00:00
abstract：:Tissue stem cells are the cell of origin for many malignancies. Metabolites regulate the balance between self-renewal and differentiation, but whether endogenous metabolic pathways or nutrient availability predispose stem cells towards transformation remains unknown. Here, we address this question in epidermal stem ce...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-020-0525-9
更新日期：2020-07-01 00:00:00
abstract：:MicroRNAs (miRNAs) have emerged as novel cancer genes. In particular, the miR-17-92 cluster, containing six individual miRNAs, is highly expressed in haematopoietic cancers and promotes lymphomagenesis in vivo. Clinical use of these findings hinges on isolating the oncogenic activity within the 17-92 cluster and defin...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2037
更新日期：2010-04-01 00:00:00
abstract：:Actin and myosin are major components of the cell cytoskeleton, with structural and regulatory functions that affect many essential cellular processes. Although they were traditionally thought to function only in the cytoplasm, it is now well accepted that actin and multiple myosins are found in the nucleus. Increasin...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb2364
更新日期：2011-11-02 00:00:00
abstract：:Proteins essential for vesicle formation by the Coat Protein I (COPI) complex are being identified, but less is known about the role of specific lipids. Brefeldin-A ADP-ribosylated substrate (BARS) functions in the fission step of COPI vesicle formation. Here, we show that BARS induces membrane curvature in cooperatio...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1774
更新日期：2008-10-01 00:00:00
abstract：:The formation of branched epithelial networks is fundamental to the development of many organs, such as the lung, the kidney or the vasculature. Little is known about the mechanisms that control cell rearrangements during tubulogenesis and regulate the size of individual tubes. Recent studies indicate that whereas the...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1049
更新日期：2003-10-01 00:00:00
abstract：:Heterochromatin performs a central role in chromosome segregation and stability by promoting cohesion at centromeres. Establishment of both heterochromatin-mediated silencing and cohesion requires passage through S phase, although the mechanism is unknown. Here we demonstrate that Schizosaccharomyces pombe Hsk1 (CDC7)...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1069
更新日期：2003-12-01 00:00:00
abstract：:Cell-cell adhesion mediated by specific cell-surface molecules is essential for multicellular development. Here we quantify de-adhesion forces at the resolution of individual cell-adhesion molecules, by controlling the interactions between single cells and combining single-molecule force spectroscopy with genetic mani...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35014000
更新日期：2000-06-01 00:00:00
abstract：:Autophagy is an evolutionarily conserved 'self-eating' process. Although the genes essential for autophagy (named Atg) have been identified in yeast, the molecular mechanism of how Atg proteins control autophagosome formation in mammalian cells remains to be elucidated. Here, we demonstrate that Bif-1 (also known as E...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1634
更新日期：2007-10-01 00:00:00
abstract：:Vaccinia virus, a close relative of the causative agent of smallpox, exploits actin polymerization to enhance its cell-to-cell spread. We show that actin-based motility of vaccinia is initiated only at the plasma membrane and remains associated with it. There must therefore be another form of cytoplasmic viral transpo...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1101-992
更新日期：2001-11-01 00:00:00
abstract：:Store-operated Ca(2+) channels (SOCs) are activated in response to Ca(2+) release from the endoplasmic reticulum (ER). The stromal interaction molecule 1 (STIM1) is the ER sensor that transmits the stored Ca(2+) content to the pore-forming SOCs Orai and TRPC channels. Recent studies reveal high levels of Orai1 and STI...
journal_title：Nature cell biology
pub_type： 新闻
doi：10.1038/ncb0608-639
更新日期：2008-06-01 00:00:00
abstract：:Phagocytic cells engulf their prey into vesicular structures called phagosomes, of which a certain proportion becomes demarcated for enhanced maturation by a process called LC3-associated phagocytosis (LAP). Light has now been shed on the molecular requirements of LAP, establishing a central role for the protein Rubic...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb3197
更新日期：2015-07-01 00:00:00
abstract：:Formins have been implicated in the regulation of cytoskeletal structure in animals and fungi. Here we show that the formins Bni1 and Bnr1 of budding yeast stimulate the assembly of actin filaments that function as precursors to tropomyosin-stabilized cables that direct polarized cell growth. With loss of formin funct...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb718
更新日期：2002-01-01 00:00:00
abstract：:The emergence of resistance to poly-ADP-ribose polymerase inhibitors (PARPi) poses a threat to the treatment of BRCA1 and BRCA2 (BRCA1/2)-deficient tumours. Stabilization of stalled DNA replication forks is a recently identified PARPi-resistance mechanism that promotes genomic stability in BRCA1/2-deficient cancers. D...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3626
更新日期：2017-11-01 00:00:00
abstract：:Reprogrammed glucose metabolism as a result of increased glycolysis and glucose uptake is a hallmark of cancer. Here we show that cancer cells can suppress glucose uptake by non-tumour cells in the premetastatic niche, by secreting vesicles that carry high levels of the miR-122 microRNA. High miR-122 levels in the cir...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3094
更新日期：2015-02-01 00:00:00
abstract：:In three-dimensional matrices cancer cells move with a rounded, amoeboid morphology that is controlled by ROCK-dependent contraction of acto-myosin. In this study, we show that PDK1 is required for phosphorylation of myosin light chain and cell motility, both on deformable gels and in vivo. Depletion of PDK1 alters th...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1675
更新日期：2008-02-01 00:00:00
abstract：:Chromophore-assisted laser inactivation (CALI) is a light-mediated technique used to selectively inactivate proteins within cells. Here, we demonstrate that GFP can be used as a CALI reagent to locally inactivate proteins in living cells. We show that focused laser irradiation of EGFP-alpha-actinin expressed in Swiss ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb772
更新日期：2002-04-01 00:00:00
abstract：:The mitogen-activated protein kinase (MAPK) network is a conserved signalling module that regulates cell fate by transducing a myriad of growth-factor signals. The ability of this network to coordinate and process a variety of inputs from different growth-factor receptors into specific biological responses is, however...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1543
更新日期：2007-03-01 00:00:00
abstract：:Organization of chromatin by epigenetic mechanisms is essential for establishing and maintaining cellular identity in developing and adult organisms. A key question that remains unresolved about this process is how epigenetic marks are transmitted to the next cell generation during cell division. Here we provide a mod...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1787
更新日期：2008-11-01 00:00:00
abstract：:Numerous cellular functions depend on actin filament (F-actin) disassembly. The best-characterized disassembly proteins, the ADF (actin-depolymerizing factor)/cofilins (encoded by the twinstar gene in Drosophila), sever filaments and recycle monomers to promote actin assembly. Cofilin is also a relatively weak actin d...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3390
更新日期：2016-08-01 00:00:00
abstract：:The actin cable is a supracellular structure that embryonic epithelia produce to close gaps. However, the action of the cable remains debated. Here, we address the function of the cable using Drosophila dorsal closure, a paradigm to understand wound healing. First, we show that the actin cytoskeleton protein Zasp52 is...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3421
更新日期：2016-11-01 00:00:00
abstract：:Aberrant activation of beta-catenin promotes cell proliferation and initiates colorectal tumorigenesis. However, the expansion of tumours and the inadequacy of their local vasculature results in areas of hypoxia where cell growth is typically constrained. Here, we report a novel diversion in beta-catenin signalling tr...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1534
更新日期：2007-02-01 00:00:00
abstract：:Treatment of cultured vertebrate neurons with nitric oxide leads to growth-cone collapse, axon retraction and the reconfiguration of axonal microtubules. We show that the light chain of microtubule-associated protein (MAP) 1B is a substrate for S-nitrosylation in vivo, in cultured cells and in vitro. S-nitrosylation o...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1625
更新日期：2007-09-01 00:00:00
abstract：:Assisted reproductive technologies (ART) are exceptional among clinical therapies, as unlike most medical procedures, ART have generational consequences. Further, human embryo research in the US has been sponsored solely by the private sector and, until recent biotechnology forays into human embryonic stem cell (hESC)...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb-nm-fertilityS19
更新日期：2002-10-01 00:00:00
abstract：:Structural maintenance of chromosomes (SMC) protein complexes are key determinants of chromosome conformation. Using Hi-C and polymer modelling, we study how cohesin and condensin, two deeply conserved SMC complexes, organize chromosomes in the budding yeast Saccharomyces cerevisiae. The canonical role of cohesin is t...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3594
更新日期：2017-09-01 00:00:00
abstract：:Extracellular signal-regulated kinase (ERK) controls fundamental cellular functions, including cell fate decisions. In PC12, cells shifting ERK activation from transient to sustained induces neuronal differentiation. As ERK associates with both regulators and effectors, we hypothesized that the mechanisms underlying t...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1994
更新日期：2009-12-01 00:00:00
