当前位置： SCI文献检索 > NATURE CELL BIOLOGY期刊下所有文献 > Kinesin-dependent movement on microtubules precedes actin-based motility of vaccinia virus.

Kinesin-dependent movement on microtubules precedes actin-based motility of vaccinia virus.

Abstract：

:Vaccinia virus, a close relative of the causative agent of smallpox, exploits actin polymerization to enhance its cell-to-cell spread. We show that actin-based motility of vaccinia is initiated only at the plasma membrane and remains associated with it. There must therefore be another form of cytoplasmic viral transport, from the cell centre, where the virus replicates, to the periphery. Video analysis reveals that GFP-labelled intracellular enveloped virus particles (IEVs) move from their perinuclear site of assembly to the plasma membrane on microtubules. We show that the viral membrane protein A36R, which is essential for actin-based motility of vaccinia, is also involved in microtubule-mediated movement of IEVs. We further show that conventional kinesin is recruited to IEVs via the light chain TPR repeats and is required for microtubule-based motility of the virus. Vaccinia thus sequentially exploits the microtubule and actin cytoskeletons to enhance its cell-to-cell spread.

journal_name

Nat Cell Biol

journal_title

Nature cell biology

authors

Rietdorf J,Ploubidou A,Reckmann I,Holmström A,Frischknecht F,Zettl M,Zimmermann T,Way M

doi

10.1038/ncb1101-992

keywords:

subject

Has Abstract

pub_date

2001-11-01 00:00:00

pages

992-1000

issue

11

eissn

1465-7392

issn

1476-4679

pii

ncb1101-992

journal_volume

3

pub_type

杂志文章

相关文献

NATURE CELL BIOLOGY文献大全
  • New protease inhibitors prevent gamma-secretase-mediated production of Abeta40/42 without affecting Notch cleavage.

    abstract：:We have designed new non-peptidic potential inhibitors of gamma-secretase and examined their ability to prevent production of amyloid-beta 40 (Abeta40) and Abeta42 by human cells expressing wild-type and Swedish-mutant beta-amyloid precursor protein (betaAPP). Here we identify three such agents that markedly reduce re...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/35074581

    authors： Petit A,Bihel F,Alvès da Costa C,Pourquié O,Checler F,Kraus JL

    更新日期：2001-05-01 00:00:00

  • Intercellular transfer to signalling endosomes regulates an ex vivo bone marrow niche.

    abstract：:Haematopoietic stem-progenitor cells (HSPCs) reside in the bone marrow niche, where interactions with osteoblasts provide essential cues for their proliferation and survival. Here, we use live-cell imaging to characterize both the site of contact between osteoblasts and haematopoietic progenitor cells (HPCs) and event...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1838

    authors： Gillette JM,Larochelle A,Dunbar CE,Lippincott-Schwartz J

    更新日期：2009-03-01 00:00:00

  • ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect.

    abstract：:Pyruvate kinase M2 (PKM2) is upregulated in multiple cancer types and contributes to the Warburg effect by unclear mechanisms. Here we demonstrate that EGFR-activated ERK2 binds directly to PKM2 Ile 429/Leu 431 through the ERK2 docking groove and phosphorylates PKM2 at Ser 37, but does not phosphorylate PKM1. Phosphor...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb2629

    authors： Yang W,Zheng Y,Xia Y,Ji H,Chen X,Guo F,Lyssiotis CA,Aldape K,Cantley LC,Lu Z

    更新日期：2012-12-01 00:00:00

  • A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly.

    abstract：:TP53 (tumour protein 53) is one of the most frequently mutated genes in human cancer and its role during cellular transformation has been studied extensively. However, the homeostatic functions of p53 are less well understood. Here, we explore the molecular dependency network of TP53 through an RNAi-mediated synthetic...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb2264

    authors： Krastev DB,Slabicki M,Paszkowski-Rogacz M,Hubner NC,Junqueira M,Shevchenko A,Mann M,Neugebauer KM,Buchholz F

    更新日期：2011-06-05 00:00:00

  • Cell plasticity in epithelial homeostasis and tumorigenesis.

    abstract：:The adult organism is characterized by remarkable plasticity, which enables efficient regeneration and restoration of homeostasis after damage. When aberrantly activated, this plasticity contributes to tumour initiation and progression. Here we review recent advances in this field with a focus on cell fate changes and...

    journal_title：Nature cell biology

    pub_type： 杂志文章,评审

    doi：10.1038/ncb3611

    authors： Varga J,Greten FR

    更新日期：2017-10-01 00:00:00

  • EGFR signalling as a negative regulator of Notch1 gene transcription and function in proliferating keratinocytes and cancer.

    abstract：:The Notch1 gene has an important role in mammalian cell-fate decision and tumorigenesis. Upstream control mechanisms for transcription of this gene are still poorly understood. In a chemical genetics screen for small molecule activators of Notch signalling, we identified epidermal growth factor receptor (EGFR) as a ke...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1750

    authors： Kolev V,Mandinova A,Guinea-Viniegra J,Hu B,Lefort K,Lambertini C,Neel V,Dummer R,Wagner EF,Dotto GP

    更新日期：2008-08-01 00:00:00

  • Author Correction: Transient Scute activation via a self-stimulatory loop directs enteroendocrine cell pair specification from self-renewing intestinal stem cells.

    abstract：:In the version of this Article originally published, the author had misnumbered the reference citations in the Methods, using numbers 1-14 instead of 46-59. These errors have now been corrected in all online versions of the Article. ...

    journal_title：Nature cell biology

    pub_type： 杂志文章,已发布勘误

    doi：10.1038/s41556-018-0053-z

    authors： Chen J,Xu N,Wang C,Huang P,Huang H,Jin Z,Yu Z,Cai T,Jiao R,Xi R

    更新日期：2018-08-01 00:00:00

  • Recruitment and activation of caspase-8 by the Huntingtin-interacting protein Hip-1 and a novel partner Hippi.

    abstract：:In Huntington disease, polyglutamine expansion of the protein huntingtin (Htt) leads to selective neurodegenerative loss of medium spiny neurons throughout the striatum by an unknown apoptotic mechanism. Binding of Hip-1, a protein normally associated with Htt, is reduced by polyglutamine expansion. Free Hip-1 binds t...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb735

    authors： Gervais FG,Singaraja R,Xanthoudakis S,Gutekunst CA,Leavitt BR,Metzler M,Hackam AS,Tam J,Vaillancourt JP,Houtzager V,Rasper DM,Roy S,Hayden MR,Nicholson DW

    更新日期：2002-02-01 00:00:00

  • Bcl-2 proteins: regulators of apoptosis or of mitochondrial homeostasis?

    abstract：:Programmed cell death (apoptosis) is used by multicellular organisms during development and to maintain homeostasis within mature tissues. One of the first genes shown to regulate apoptosis was bcl-2. Subsequently, a number of Bcl-2-related proteins have been identified. Despite overwhelming evidence that Bcl-2 protei...

    journal_title：Nature cell biology

    pub_type： 杂志文章,评审

    doi：10.1038/70237

    authors： Vander Heiden MG,Thompson CB

    更新日期：1999-12-01 00:00:00

  • EZH2 promotes degradation of stalled replication forks by recruiting MUS81 through histone H3 trimethylation.

    abstract：:The emergence of resistance to poly-ADP-ribose polymerase inhibitors (PARPi) poses a threat to the treatment of BRCA1 and BRCA2 (BRCA1/2)-deficient tumours. Stabilization of stalled DNA replication forks is a recently identified PARPi-resistance mechanism that promotes genomic stability in BRCA1/2-deficient cancers. D...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb3626

    authors： Rondinelli B,Gogola E,Yücel H,Duarte AA,van de Ven M,van der Sluijs R,Konstantinopoulos PA,Jonkers J,Ceccaldi R,Rottenberg S,D'Andrea AD

    更新日期：2017-11-01 00:00:00

  • A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance.

    abstract：:Proteins essential for vesicle formation by the Coat Protein I (COPI) complex are being identified, but less is known about the role of specific lipids. Brefeldin-A ADP-ribosylated substrate (BARS) functions in the fission step of COPI vesicle formation. Here, we show that BARS induces membrane curvature in cooperatio...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1774

    authors： Yang JS,Gad H,Lee SY,Mironov A,Zhang L,Beznoussenko GV,Valente C,Turacchio G,Bonsra AN,Du G,Baldanzi G,Graziani A,Bourgoin S,Frohman MA,Luini A,Hsu VW

    更新日期：2008-10-01 00:00:00

  • Immuno-subtyping of breast cancer reveals distinct myeloid cell profiles and immunotherapy resistance mechanisms.

    abstract：:Cancer-induced immune responses affect tumour progression and therapeutic response. In multiple murine models and clinical datasets, we identified large variations of neutrophils and macrophages that define 'immune subtypes' of triple-negative breast cancer (TNBC), including neutrophil-enriched (NES) and macrophage-en...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/s41556-019-0373-7

    authors： Kim IS,Gao Y,Welte T,Wang H,Liu J,Janghorban M,Sheng K,Niu Y,Goldstein A,Zhao N,Bado I,Lo HC,Toneff MJ,Nguyen T,Bu W,Jiang W,Arnold J,Gu F,He J,Jebakumar D,Walker K,Li Y,Mo Q,Westbrook TF,Zong C,Rao A,

    更新日期：2019-09-01 00:00:00

  • Invasive cell migration is initiated by guided growth of long cellular extensions.

    abstract：:The migration of border cells during Drosophila melanogaster oogenesis is a simple and powerful system for studying invasive cell migration in vivo. Border cells are somatic cells that delaminate from the follicular epithelium of an egg chamber and invade the germ line cluster. They migrate between the nurse cells to ...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb848

    authors： Fulga TA,Rørth P

    更新日期：2002-09-01 00:00:00

  • An interaction between Scribble and the NADPH oxidase complex controls M1 macrophage polarization and function.

    abstract：:The polarity protein Scribble (SCRIB) regulates apical-basal polarity, directional migration and tumour suppression in Drosophila and mammals. Here we report that SCRIB is an important regulator of myeloid cell functions including bacterial infection and inflammation. SCRIB interacts directly with the NADPH oxidase (N...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb3413

    authors： Zheng W,Umitsu M,Jagan I,Tran CW,Ishiyama N,BeGora M,Araki K,Ohashi PS,Ikura M,Muthuswamy SK

    更新日期：2016-11-01 00:00:00

  • Targeting the perivascular niche sensitizes disseminated tumour cells to chemotherapy.

    abstract：:The presence of disseminated tumour cells (DTCs) in bone marrow is predictive of poor metastasis-free survival of patients with breast cancer with localized disease. DTCs persist in distant tissues despite systemic administration of adjuvant chemotherapy. Many assume that this is because the majority of DTCs are quies...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/s41556-018-0267-0

    authors： Carlson P,Dasgupta A,Grzelak CA,Kim J,Barrett A,Coleman IM,Shor RE,Goddard ET,Dai J,Schweitzer EM,Lim AR,Crist SB,Cheresh DA,Nelson PS,Hansen KC,Ghajar CM

    更新日期：2019-02-01 00:00:00

  • Extracellular serine controls epidermal stem cell fate and tumour initiation.

    abstract：:Tissue stem cells are the cell of origin for many malignancies. Metabolites regulate the balance between self-renewal and differentiation, but whether endogenous metabolic pathways or nutrient availability predispose stem cells towards transformation remains unknown. Here, we address this question in epidermal stem ce...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/s41556-020-0525-9

    authors： Baksh SC,Todorova PK,Gur-Cohen S,Hurwitz B,Ge Y,Novak JSS,Tierney MT,Dela Cruz-Racelis J,Fuchs E,Finley LWS

    更新日期：2020-07-01 00:00:00

  • The NSL complex maintains nuclear architecture stability via lamin A/C acetylation.

    abstract：:While nuclear lamina abnormalities are hallmarks of human diseases, their interplay with epigenetic regulators and precise epigenetic landscape remain poorly understood. Here, we show that loss of the lysine acetyltransferase MOF or its associated NSL-complex members KANSL2 or KANSL3 leads to a stochastic accumulation...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/s41556-019-0397-z

    authors： Karoutas A,Szymanski W,Rausch T,Guhathakurta S,Rog-Zielinska EA,Peyronnet R,Seyfferth J,Chen HR,de Leeuw R,Herquel B,Kimura H,Mittler G,Kohl P,Medalia O,Korbel JO,Akhtar A

    更新日期：2019-10-01 00:00:00

  • Endocytic proteins are partitioned at the edge of the clathrin lattice in mammalian cells.

    abstract：:Dozens of proteins capture, polymerize and reshape the clathrin lattice during clathrin-mediated endocytosis (CME). How or if this ensemble of proteins is organized in relation to the clathrin coat is unknown. Here, we map key molecules involved in CME at the nanoscale using correlative super-resolution light and tran...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb3498

    authors： Sochacki KA,Dickey AM,Strub MP,Taraska JW

    更新日期：2017-04-01 00:00:00

  • Telomere-independent Rap1 is an IKK adaptor and regulates NF-kappaB-dependent gene expression.

    abstract：:We describe a genome-wide gain-of-function screen for regulators of NF-kappaB, and identify Rap1 (Trf2IP), as an essential modulator of NF-kappaB-mediated pathways. NF-kappaB is induced by ectopic expression of Rap1, whereas its activity is inhibited by Rap1 depletion. In addition to localizing on telomeres, mammalian...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb2080

    authors： Teo H,Ghosh S,Luesch H,Ghosh A,Wong ET,Malik N,Orth A,de Jesus P,Perry AS,Oliver JD,Tran NL,Speiser LJ,Wong M,Saez E,Schultz P,Chanda SK,Verma IM,Tergaonkar V

    更新日期：2010-08-01 00:00:00

  • PML regulates p53 stability by sequestering Mdm2 to the nucleolus.

    abstract：:The promyelocytic leukaemia (PML) tumour-suppressor protein potentiates p53 function by regulating post-translational modifications, such as CBP-dependent acetylation and Chk2-dependent phosphorylation, in the PML-Nuclear Body (NB). PML was recently shown to interact with the p53 ubiquitin-ligase Mdm2 (refs 4-6); howe...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1147

    authors： Bernardi R,Scaglioni PP,Bergmann S,Horn HF,Vousden KH,Pandolfi PP

    更新日期：2004-07-01 00:00:00

  • Kinetochore motors drive congression of peripheral polar chromosomes by overcoming random arm-ejection forces.

    abstract：:Accurate chromosome segregation during cell division in metazoans relies on proper chromosome congression at the equator. Chromosome congression is achieved after bi-orientation to both spindle poles shortly after nuclear envelope breakdown, or by the coordinated action of motor proteins that slide misaligned chromoso...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb3060

    authors： Barisic M,Aguiar P,Geley S,Maiato H

    更新日期：2014-12-01 00:00:00

  • Rab10 GTPase regulates ER dynamics and morphology.

    abstract：:We have identified Rab10 as an ER-specific Rab GTPase that regulates ER structure and dynamics. We show that Rab10 localizes to the ER and to dynamic ER-associated structures that track along microtubules and mark the position of new ER tubule growth. Rab10 depletion or expression of a Rab10 GDP-locked mutant alters E...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb2647

    authors： English AR,Voeltz GK

    更新日期：2013-02-01 00:00:00

  • Reaper eliminates IAP proteins through stimulated IAP degradation and generalized translational inhibition.

    abstract：:Inhibitors of apoptosis (IAPs) inhibit caspases, thereby preventing proteolysis of apoptotic substrates. IAPs occlude the active sites of caspases to which they are bound and can function as ubiquitin ligases. IAPs are also reported to ubiquitinate themselves and caspases. Several proteins induce apoptosis, at least i...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb798

    authors： Holley CL,Olson MR,Colón-Ramos DA,Kornbluth S

    更新日期：2002-06-01 00:00:00

  • Regulation of ubiquitin-binding proteins by monoubiquitination.

    abstract：:Proteins containing ubiquitin-binding domains (UBDs) interact with ubiquitinated targets and regulate diverse biological processes, including endocytosis, signal transduction, transcription and DNA repair. Many of the UBD-containing proteins are also themselves monoubiquitinated, but the functional role and the mechan...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1354

    authors： Hoeller D,Crosetto N,Blagoev B,Raiborg C,Tikkanen R,Wagner S,Kowanetz K,Breitling R,Mann M,Stenmark H,Dikic I

    更新日期：2006-02-01 00:00:00

  • Vesicles modulate an actin network for asymmetric spindle positioning.

    abstract：:Actin networks drive many essential cellular processes, including cell migration, cytokinesis and tissue morphogenesis. However, how cells organize and regulate dynamic actin networks that consist of long, unbranched actin filaments is only poorly understood. This study in mouse oocytes reveals that cells can use vesi...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb2802

    authors： Holubcová Z,Howard G,Schuh M

    更新日期：2013-08-01 00:00:00

  • AMPK and PFKFB3 mediate glycolysis and survival in response to mitophagy during mitotic arrest.

    abstract：:Blocking mitotic progression has been proposed as an attractive therapeutic strategy to impair proliferation of tumour cells. However, how cells survive during prolonged mitotic arrest is not well understood. We show here that survival during mitotic arrest is affected by the special energetic requirements of mitotic ...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb3231

    authors： Doménech E,Maestre C,Esteban-Martínez L,Partida D,Pascual R,Fernández-Miranda G,Seco E,Campos-Olivas R,Pérez M,Megias D,Allen K,López M,Saha AK,Velasco G,Rial E,Méndez R,Boya P,Salazar-Roa M,Malumbres M

    更新日期：2015-10-01 00:00:00

  • Drosophila CLASP is required for the incorporation of microtubule subunits into fluxing kinetochore fibres.

    abstract：:The motion of a chromosome during mitosis is mediated by a bundle of microtubules, termed a kinetochore fibre (K-fibre), which connects the kinetochore of the chromosome to a spindle pole. Once formed, mature K-fibres maintain a steady state length because the continuous addition of microtubule subunits onto microtubu...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1207

    authors： Maiato H,Khodjakov A,Rieder CL

    更新日期：2005-01-01 00:00:00

  • The endogenous ligand Stunted of the GPCR Methuselah extends lifespan in Drosophila.

    abstract：:Many extracellular signals are transmitted to the interior of the cell by receptors with seven membrane-spanning helices that trigger their effects by means of heterotrimeric guanine-nucleotide-binding regulatory proteins (G proteins). These G-protein-coupled receptors (GPCRs) control various physiological functions i...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1133

    authors： Cvejic S,Zhu Z,Felice SJ,Berman Y,Huang XY

    更新日期：2004-06-01 00:00:00

  • Dynamics and retention of misfolded proteins in native ER membranes.

    abstract：:When co-translationally inserted into endoplasmic reticulum (ER) membranes, newly synthesized proteins encounter the lumenal environment of the ER, which contains chaperone proteins that facilitate the folding reactions necessary for protein oligomerization, maturation and export from the ER. Here we show, using a tem...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/35010558

    authors： Nehls S,Snapp EL,Cole NB,Zaal KJ,Kenworthy AK,Roberts TH,Ellenberg J,Presley JF,Siggia E,Lippincott-Schwartz J

    更新日期：2000-05-01 00:00:00

  • Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling.

    abstract：:Loss-of-function (LOF) mutations in the endothelial cell (EC)-enriched gene endoglin (ENG) cause the human disease hereditary haemorrhagic telangiectasia-1, characterized by vascular malformations promoted by vascular endothelial growth factor A (VEGFA). How ENG deficiency alters EC behaviour to trigger these anomalie...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb3534

    authors： Jin Y,Muhl L,Burmakin M,Wang Y,Duchez AC,Betsholtz C,Arthur HM,Jakobsson L

    更新日期：2017-06-01 00:00:00