Invasive cell migration is initiated by guided growth of long cellular extensions.

abstract：:Formation of a bipolar spindle is essential for faithful chromosome segregation at mitosis. Because centrosomes define spindle poles, defects in centrosome number and structural organization can lead to a loss of bipolarity. In addition, microtubule-mediated pulling and pushing forces acting on centrosomes and chromos...

journal_title：Nature cell biology

authors： Oshimori N,Ohsugi M,Yamamoto T

更新日期：2006-10-01 00:00:00

abstract：:The resolution of joint molecules that link recombining sister chromatids is essential for chromosome segregation. Here, we determine the fate of unresolved recombination intermediates arising in cells lacking two nucleases required for resolution (GEN1 -/- knockout cells depleted of MUS81). We find that intermediates...

journal_title：Nature cell biology

authors： Chan YW,Fugger K,West SC

更新日期：2018-01-01 00:00:00

abstract：:Pyruvate kinase M2 (PKM2) is upregulated in multiple cancer types and contributes to the Warburg effect by unclear mechanisms. Here we demonstrate that EGFR-activated ERK2 binds directly to PKM2 Ile 429/Leu 431 through the ERK2 docking groove and phosphorylates PKM2 at Ser 37, but does not phosphorylate PKM1. Phosphor...

journal_title：Nature cell biology

authors： Yang W,Zheng Y,Xia Y,Ji H,Chen X,Guo F,Lyssiotis CA,Aldape K,Cantley LC,Lu Z

更新日期：2012-12-01 00:00:00

abstract：:Cellular transformation and cancer progression is accompanied by changes in the metabolic landscape. Master co-regulators of metabolism orchestrate the modulation of multiple metabolic pathways through transcriptional programs, and hence constitute a probabilistically parsimonious mechanism for general metabolic rewir...

journal_title：Nature cell biology

authors： Torrano V,Valcarcel-Jimenez L,Cortazar AR,Liu X,Urosevic J,Castillo-Martin M,Fernández-Ruiz S,Morciano G,Caro-Maldonado A,Guiu M,Zúñiga-García P,Graupera M,Bellmunt A,Pandya P,Lorente M,Martín-Martín N,Sutherland JD,San

更新日期：2016-06-01 00:00:00

abstract：:Adenomatous polyposis coli protein (APC) is an important tumour suppressor in the human colon epithelium. In a complex with glycogen synthase kinase-3 (GSK-3), APC binds to and destabilizes cytoplasmic ('free') beta-catenin. Here, using a yeast two-hybrid screen for proteins that bind to the Drosophila beta-catenin ho...

journal_title：Nature cell biology

authors： Yu X,Waltzer L,Bienz M

更新日期：1999-07-01 00:00:00

abstract：:Lamina-associated polypeptide (LAP) 2alpha is a chromatin-associated protein that binds A-type lamins. Mutations in both LAP2alpha and A-type lamins are linked to human diseases called laminopathies, but the molecular mechanisms are poorly understood. The A-type lamin-LAP2alpha complex interacts with and regulates ret...

journal_title：Nature cell biology

authors： Naetar N,Korbei B,Kozlov S,Kerenyi MA,Dorner D,Kral R,Gotic I,Fuchs P,Cohen TV,Bittner R,Stewart CL,Foisner R

更新日期：2008-11-01 00:00:00

abstract：:Phagocytic cells engulf their prey into vesicular structures called phagosomes, of which a certain proportion becomes demarcated for enhanced maturation by a process called LC3-associated phagocytosis (LAP). Light has now been shed on the molecular requirements of LAP, establishing a central role for the protein Rubic...

journal_title：Nature cell biology

authors： Boyle KB,Randow F

更新日期：2015-07-01 00:00:00

abstract：:Once considered to be contained, infectious diseases of bacterial origin are now making a comeback. A lack of innovative therapies and the appearance of drug-resistant pathogens are becoming increasingly serious problems. A better understanding of pathogen-host interactions at the cellular and molecular levels is nece...

journal_title：Nature cell biology

authors： Méresse S,Steele-Mortimer O,Moreno E,Desjardins M,Finlay B,Gorvel JP

更新日期：1999-11-01 00:00:00

abstract：:Loss-of-function (LOF) mutations in the endothelial cell (EC)-enriched gene endoglin (ENG) cause the human disease hereditary haemorrhagic telangiectasia-1, characterized by vascular malformations promoted by vascular endothelial growth factor A (VEGFA). How ENG deficiency alters EC behaviour to trigger these anomalie...

journal_title：Nature cell biology

authors： Jin Y,Muhl L,Burmakin M,Wang Y,Duchez AC,Betsholtz C,Arthur HM,Jakobsson L

更新日期：2017-06-01 00:00:00

abstract：:Metazoan internal organs are assembled from polarized tubular epithelia that must set aside an apical membrane domain as a lumenal surface. In a global Caenorhabditis elegans tubulogenesis screen, interference with several distinct fatty-acid-biosynthetic enzymes transformed a contiguous central intestinal lumen into ...

journal_title：Nature cell biology

authors： Zhang H,Abraham N,Khan LA,Hall DH,Fleming JT,Göbel V

更新日期：2011-09-18 00:00:00

abstract：:Chromosome missegregation into a micronucleus can cause complex and localized genomic rearrangements known as chromothripsis, but the underlying mechanisms remain unresolved. Here we developed an inducible Y centromere-selective inactivation strategy by exploiting a CENP-A/histone H3 chimaera to directly examine the f...

journal_title：Nature cell biology

authors： Ly P,Teitz LS,Kim DH,Shoshani O,Skaletsky H,Fachinetti D,Page DC,Cleveland DW

更新日期：2017-01-01 00:00:00

abstract：:Coordination of microtubules and the actin cytoskeleton is important in several types of cell movement. mDia1 is a member of the formin-homology family of proteins and an effector of the small GTPase Rho. It contains the Rho-binding domain in its amino terminus and two distinct regions of formin homology, FH1 in the m...

journal_title：Nature cell biology

authors： Ishizaki T,Morishima Y,Okamoto M,Furuyashiki T,Kato T,Narumiya S

更新日期：2001-01-01 00:00:00

abstract：:Cancer cells use aerobic glycolysis preferentially for energy provision and this metabolic change is important for tumour growth. Here, we have found a link between the tumour suppressor p53, the transcription factor NF-kappaB and glycolysis. In p53-deficient primary cultured cells, kinase activities of IKKalpha and I...

journal_title：Nature cell biology

authors： Kawauchi K,Araki K,Tobiume K,Tanaka N

更新日期：2008-05-01 00:00:00

abstract：:Activation of mitosis-promoting factor (MPF) drives mitotic commitment. In human cells active MPF appears first on centrosomes. We show that local activation of MPF on the equivalent organelle of fission yeast, the spindle pole body (SPB), promotes Polo kinase activity at the SPBs long before global MPF activation dri...

journal_title：Nature cell biology

authors： Grallert A,Patel A,Tallada VA,Chan KY,Bagley S,Krapp A,Simanis V,Hagan IM

更新日期：2013-01-01 00:00:00

abstract：:The actin cytoskeleton has been implicated in restricting diffusion of plasma membrane components. Here, simultaneous observations of quantum dot-labelled FcepsilonRI motion and GFP-tagged actin dynamics provide direct evidence that actin filament bundles define micron-sized domains that confine mobile receptors. Dyna...

journal_title：Nature cell biology

authors： Andrews NL,Lidke KA,Pfeiffer JR,Burns AR,Wilson BS,Oliver JM,Lidke DS

更新日期：2008-08-01 00:00:00

abstract：:Chromophore-assisted laser inactivation (CALI) is a light-mediated technique used to selectively inactivate proteins within cells. Here, we demonstrate that GFP can be used as a CALI reagent to locally inactivate proteins in living cells. We show that focused laser irradiation of EGFP-alpha-actinin expressed in Swiss ...

journal_title：Nature cell biology

authors： Rajfur Z,Roy P,Otey C,Romer L,Jacobson K

更新日期：2002-04-01 00:00:00

abstract：:Actin and myosin are major components of the cell cytoskeleton, with structural and regulatory functions that affect many essential cellular processes. Although they were traditionally thought to function only in the cytoplasm, it is now well accepted that actin and multiple myosins are found in the nucleus. Increasin...

journal_title：Nature cell biology

authors： de Lanerolle P,Serebryannyy L

更新日期：2011-11-02 00:00:00

abstract：:Actin dynamics is a tightly regulated process involved in various cellular events including biogenesis of clathrin-coated, AP-1 (adaptor protein 1)-coated transport carriers connecting the trans-Golgi network (TGN) and the endocytic pathway. However, the mechanisms coordinating coat assembly, membrane and actin remode...

journal_title：Nature cell biology

authors： Anitei M,Stange C,Parshina I,Baust T,Schenck A,Raposo G,Kirchhausen T,Hoflack B

更新日期：2010-04-01 00:00:00

abstract：:Wiskott-Aldrich syndrome protein (WASP) and N-WASP have emerged as key proteins connecting signalling cascades to actin polymerization. Here we show that the amino-terminal WH1 domain, and not the polyproline-rich region, of N-WASP is responsible for its recruitment to sites of actin polymerization during Cdc42-indepe...

journal_title：Nature cell biology

authors： Moreau V,Frischknecht F,Reckmann I,Vincentelli R,Rabut G,Stewart D,Way M

更新日期：2000-07-01 00:00:00

abstract：:During apoptosis, dying cells are swiftly removed by phagocytes. It is not fully understood how apoptotic cells are recognized by phagocytes. Here we report the identification and characterization of the Caenorhabditis elegans ttr-52 gene, which encodes a transthyretin-like protein and is required for efficient cell c...

journal_title：Nature cell biology

authors： Wang X,Li W,Zhao D,Liu B,Shi Y,Chen B,Yang H,Guo P,Geng X,Shang Z,Peden E,Kage-Nakadai E,Mitani S,Xue D

更新日期：2010-07-01 00:00:00

abstract：:Cancer-associated fibroblasts (CAFs) promote tumour invasion and metastasis. We show that CAFs exert a physical force on cancer cells that enables their collective invasion. Force transmission is mediated by a heterophilic adhesion involving N-cadherin at the CAF membrane and E-cadherin at the cancer cell membrane. Th...

journal_title：Nature cell biology

authors： Labernadie A,Kato T,Brugués A,Serra-Picamal X,Derzsi S,Arwert E,Weston A,González-Tarragó V,Elosegui-Artola A,Albertazzi L,Alcaraz J,Roca-Cusachs P,Sahai E,Trepat X

更新日期：2017-03-01 00:00:00

abstract：:Cytokinesis requires the spatio-temporal coordination of cell-cycle control and cytoskeletal reorganization. Members of the Rho-family of GTPases are crucial regulators of this process and assembly of the contractile ring depends on local activation of Rho signalling. Here, we show that the armadillo protein p0071, un...

journal_title：Nature cell biology

authors： Wolf A,Keil R,Götzl O,Mun A,Schwarze K,Lederer M,Hüttelmaier S,Hatzfeld M

更新日期：2006-12-01 00:00:00

abstract：:Chromatin structure is thought to play a critical role in gene expression. Using the lac operator/repressor system and two colour variants of green fluorescent protein (GFP), we developed a system to visualize a gene and its protein product directly in living cells, allowing us to examine the spatial organization and ...

journal_title：Nature cell biology

authors： Tsukamoto T,Hashiguchi N,Janicki SM,Tumbar T,Belmont AS,Spector DL

更新日期：2000-12-01 00:00:00

abstract：:When cells enter mitosis with DNA that is unfit to be segregated, the consequences appear to be loss of centrosome function, abnormal spindles and a failure to segregate chromosomes. These defects may result from the workings of a surveillance mechanism that acts to cull irreparable nuclei. ...

journal_title：Nature cell biology

authors： Su TT,Vidwans SJ

更新日期：2000-02-01 00:00:00

abstract：:Vital organs such as the pancreas and the brain lack the capacity for effective regeneration. To overcome this limitation, an emerging strategy consists of converting resident tissue-specific cells into the cell types that are lost due to disease by a process called in vivo lineage reprogramming. Here we discuss recen...

journal_title：Nature cell biology

authors： Heinrich C,Spagnoli FM,Berninger B

更新日期：2015-03-01 00:00:00

abstract：:Stat3 has been shown to regulate lysosome membrane permeabilization and cell death in vivo during post-lactation mammary gland involution. It is now revealed that Stat3 induces lysosome membrane permeabilization by causing phagocytosis of milk fat globules, which destabilize the lysosome membrane leading to leakage of...

journal_title：Nature cell biology

authors： Krishna S,Overholtzer M

更新日期：2014-11-01 00:00:00

abstract：:Canonical Wnt signalling has been implicated in mouse and human embryonic stem cell (ESC) maintenance; however, its requirement is controversial. β-catenin is the key component in this highly conserved Wnt pathway, acting as a transcriptional transactivator. However, β-catenin has additional roles at the plasma membra...

journal_title：Nature cell biology

authors： Lyashenko N,Winter M,Migliorini D,Biechele T,Moon RT,Hartmann C

更新日期：2011-06-19 00:00:00

abstract：:Endocytosis of cell surface receptors is an important regulatory event in signal transduction. The transforming growth factor beta (TGF-beta) superfamily signals to the Smad pathway through heteromeric Ser-Thr kinase receptors that are rapidly internalized and then downregulated in a ubiquitin-dependent manner. Here w...

journal_title：Nature cell biology

authors： Di Guglielmo GM,Le Roy C,Goodfellow AF,Wrana JL

更新日期：2003-05-01 00:00:00

abstract：:Cell-cell adhesion mediated by specific cell-surface molecules is essential for multicellular development. Here we quantify de-adhesion forces at the resolution of individual cell-adhesion molecules, by controlling the interactions between single cells and combining single-molecule force spectroscopy with genetic mani...

journal_title：Nature cell biology

authors： Benoit M,Gabriel D,Gerisch G,Gaub HE

更新日期：2000-06-01 00:00:00

abstract：:Bcl-2 stimulates mutagenesis after the exposure of cells to DNA-damaging agents. However, the biological mechanisms of Bcl-2-mediated mutagenesis have remained largely obscure. Here we demonstrate that the Bcl-2-mediated suppression of hMSH2 expression results in a reduced cellular capacity to repair mismatches. The p...

journal_title：Nature cell biology

authors： Youn CK,Cho HJ,Kim SH,Kim HB,Kim MH,Chang IY,Lee JS,Chung MH,Hahm KS,You HJ

更新日期：2005-02-01 00:00:00