Abstract：
:Treatment of cultured vertebrate neurons with nitric oxide leads to growth-cone collapse, axon retraction and the reconfiguration of axonal microtubules. We show that the light chain of microtubule-associated protein (MAP) 1B is a substrate for S-nitrosylation in vivo, in cultured cells and in vitro. S-nitrosylation occurs at Cys 2457 in the COOH terminus. Nitrosylation of MAP1B leads to enhanced interaction with microtubules and correlates with the inhibition of neuroblastoma cell differentiation. We further show, in dorsal root ganglion neurons, that MAP1B is necessary for neuronal nitric oxide synthase control of growth-cone size, growth-cone collapse and axon retraction. These results reveal an S-nitrosylation-dependent signal-transduction pathway that is involved in regulation of the axonal cytoskeleton and identify MAP1B as a major component of this pathway. We propose that MAP1B acts by inhibiting a microtubule- and dynein-based mechanism that normally prevents axon retraction.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Stroissnigg H,Trancíková A,Descovich L,Fuhrmann J,Kutschera W,Kostan J,Meixner A,Nothias F,Propst Fdoi
10.1038/ncb1625subject
Has Abstractpub_date
2007-09-01 00:00:00pages
1035-45issue
9eissn
1465-7392issn
1476-4679pii
ncb1625journal_volume
9pub_type
杂志文章abstract：:The Morphogenesis and Cell Behaviour meeting held this fall in Barcelona explored the role of forces, adhesion and oscillations in embryonic morphogenesis. It highlighted the impact of new microscopy methods and modelling approaches on the most recent advances of the field. ...
journal_title：Nature cell biology
pub_type：
doi：10.1038/ncb0109-3
更新日期：2009-01-01 00:00:00
abstract：:Formation of a stable lamellipodium at the front of migrating cells requires localization of Rac activation to the leading edge. Restriction of alpha4 integrin phosphorylation to the leading edge limits the interaction of alpha4 with paxillin to the sides and rear of a migrating cell. The alpha4-paxillin complex inhib...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1234
更新日期：2005-04-01 00:00:00
abstract：:Here we show that human Zeste White 10 (Zw10) and Rough deal (Rod) are new components of the mitotic checkpoint, as cells lacking these proteins at kinetochores fail to arrest in mitosis when exposed to microtubule inhibitors. Checkpoint failure and premature mitotic exit may explain why cells defective for hZw10 and ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35046598
更新日期：2000-12-01 00:00:00
abstract：:We have identified Rab10 as an ER-specific Rab GTPase that regulates ER structure and dynamics. We show that Rab10 localizes to the ER and to dynamic ER-associated structures that track along microtubules and mark the position of new ER tubule growth. Rab10 depletion or expression of a Rab10 GDP-locked mutant alters E...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2647
更新日期：2013-02-01 00:00:00
abstract：:The ATR checkpoint kinase coordinates cellular responses to DNA replication stress. Budding yeast contain three activators of Mec1 (the ATR orthologue); however, only TOPBP1 is known to activate ATR in vertebrates. We identified ETAA1 as a replication stress response protein in two proteomic screens. ETAA1-deficient c...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3415
更新日期：2016-11-01 00:00:00
abstract：:NF-kappa B/Rel transcription factors control apoptosis, also known as programmed cell death. This control is crucial for oncogenesis, cancer chemo-resistance and for antagonizing tumour necrosis factor alpha (TNFalpha)-induced killing. With regard to TNFalpha, the anti-apoptotic activity of NF-kappa B involves suppres...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1093
更新日期：2004-02-01 00:00:00
abstract：:For accurate segregation of chromosomes during cell division, microtubule fibres must attach sister kinetochores to opposite poles of the mitotic spindle (bi-orientation). Aurora kinases are linked to oncogenesis and have been implicated in the regulation of chromosome-microtubule attachments. Although loss of Aurora ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1102
更新日期：2004-03-01 00:00:00
abstract：:Only a few RFamide peptides have been identified in mammals, although they have been abundantly found in invertebrates. Here we report the identification of a human gene that encodes at least three RFamide-related peptides, hRFRP-1-3. Cells transfected with a seven-transmembrane-domain receptor, OT7T022, specifically ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35036326
更新日期：2000-10-01 00:00:00
abstract：:The SNF2h (sucrose non-fermenting protein 2 homologue)-containing chromatin-remodelling complex NoRC silences a fraction of ribosomal RNA genes (rDNA) by establishing a heterochromatic structure at the rDNA promoter. Here we show that the acetyltransferase MOF (males absent on the first) acetylates TIP5, the largest s...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1914
更新日期：2009-08-01 00:00:00
abstract：:Neural Wiskott-Aldrich syndrome protein (N-WASP) and WAVE are members of a family of proteins that use the Arp2/3 complex to stimulate actin assembly in actin-based motile processes. By entering into distinct macromolecular complexes, they act as convergent nodes of different signalling pathways. The role of WAVE in g...
journal_title：Nature cell biology
pub_type： 信件
doi：10.1038/ncb1304
更新日期：2005-10-01 00:00:00
abstract：:To maintain genome function and stability, DNA sequence and its organization into chromatin must be duplicated during cell division. Understanding how entire chromosomes are copied remains a major challenge. Here, we use nascent chromatin capture (NCC) to profile chromatin proteome dynamics during replication in human...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2918
更新日期：2014-03-01 00:00:00
abstract：:The store-operated calcium channel (SOC) located in the plasma membrane (PM) mediates capacitative entry of extracellular calcium after depletion of intracellular calcium stores in the endoplasmic or sarcoplasmic reticulum (ER/SR). An intimate interaction between the PM and the ER/SR is essential for the operation of ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb788
更新日期：2002-05-01 00:00:00
abstract：:In the version of this Article originally published, the author had misnumbered the reference citations in the Methods, using numbers 1-14 instead of 46-59. These errors have now been corrected in all online versions of the Article. ...
journal_title：Nature cell biology
pub_type： 杂志文章,已发布勘误
doi：10.1038/s41556-018-0053-z
更新日期：2018-08-01 00:00:00
abstract：:The endoplasmic reticulum (ER), a network of membranous sheets and pipes, supports functions encompassing biogenesis of secretory proteins and delivery of functional solutes throughout the cell1,2. Molecular mobility through the ER network enables these functionalities, but diffusion alone is not sufficient to explain...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-018-0192-2
更新日期：2018-10-01 00:00:00
abstract：:Chromosome missegregation into a micronucleus can cause complex and localized genomic rearrangements known as chromothripsis, but the underlying mechanisms remain unresolved. Here we developed an inducible Y centromere-selective inactivation strategy by exploiting a CENP-A/histone H3 chimaera to directly examine the f...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3450
更新日期：2017-01-01 00:00:00
abstract：:Cadherin-containing cell-cell junctions respond to intercellular tension by increasing their size, strength and complexity. The mechanical regulation of cadherin adhesions is now shown to involve myosin-dependent tension in the cortical actomyosin cytoskeleton. This reduces actin turnover to decrease the mobility of c...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb2980
更新日期：2014-06-01 00:00:00
abstract：:During the development of multicellular organisms, concerted actions of molecular signalling networks determine whether cells undergo proliferation, differentiation, death or ageing. Here we show that genetic inactivation of the stress signalling kinase, MKK7, a direct activator of JNKs in mice, results in embryonic l...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1098
更新日期：2004-03-01 00:00:00
abstract：:The AP-1 transcription factor c-Jun is essential for cellular proliferation in many cell types, but the molecular link between growth factors and c-Jun activation has been enigmatic. In this study we identify a previously uncharacterized RING-domain-containing protein, RACO-1 (RING domain AP-1 co-activator-1), as a c-...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2098
更新日期：2010-10-01 00:00:00
abstract：:Localization and activation of heterotrimeric G proteins have a crucial role during asymmetric cell division. The asymmetric division of the Drosophila sensory precursor cell (pl) is polarized along the antero-posterior axis by Frizzled signalling and, during this division, activation of Galphai depends on Partner of ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1319
更新日期：2005-11-01 00:00:00
abstract：:Inhibitors of apoptosis (IAPs) inhibit caspases, thereby preventing proteolysis of apoptotic substrates. IAPs occlude the active sites of caspases to which they are bound and can function as ubiquitin ligases. IAPs are also reported to ubiquitinate themselves and caspases. Several proteins induce apoptosis, at least i...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb798
更新日期：2002-06-01 00:00:00
abstract：:Vaccinia virus, a close relative of the causative agent of smallpox, exploits actin polymerization to enhance its cell-to-cell spread. We show that actin-based motility of vaccinia is initiated only at the plasma membrane and remains associated with it. There must therefore be another form of cytoplasmic viral transpo...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1101-992
更新日期：2001-11-01 00:00:00
abstract：:MicroRNAs (miRNAs) are increasingly implicated in regulating the malignant progression of cancer. Here we show that miR-9, which is upregulated in breast cancer cells, directly targets CDH1, the E-cadherin-encoding messenger RNA, leading to increased cell motility and invasiveness. miR-9-mediated E-cadherin downregula...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2024
更新日期：2010-03-01 00:00:00
abstract：:Integrin-containing focal adhesions transmit extracellular signals across the plasma membrane to modulate cell adhesion, signalling and survival. Although integrins are known to undergo continuous endo/exocytic traffic, the potential impact of endocytic traffic on integrin-induced signals is unknown. Here, we demonstr...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3250
更新日期：2015-11-01 00:00:00
abstract：:The actin filament (F-actin) cytoskeleton associates dynamically with the plasma membrane and is thus ideally positioned to participate in endocytosis. Indeed, a wealth of genetic and biochemical evidence has confirmed that actin interacts with components of the endocytic machinery, although its precise function in en...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1025
更新日期：2003-08-01 00:00:00
abstract：:In the unicellular eukaryote Saccharomyces cerevisiae, Cln3-cyclin-dependent kinase activity enables Start, the irreversible commitment to the cell division cycle. However, the concentration of Cln3 has been paradoxically considered to remain constant during G1, due to the presumed scaling of its production rate with ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-019-0413-3
更新日期：2019-11-01 00:00:00
abstract：:Science denial is not new but, in the digital age, evidence-based conclusions appear to be increasingly threatened by beliefs based on emotion and isolated personal experience. When confronting a post-truth world, scientists must defend the scientific method and increase public engagement. ...
journal_title：Nature cell biology
pub_type： 社论
doi：10.1038/s41556-018-0231-z
更新日期：2018-11-01 00:00:00
abstract：:Although myosin II filaments are known to exist in non-muscle cells, their dynamics and organization are incompletely understood. Here, we combined structured illumination microscopy with pharmacological and genetic perturbations, to study the process of actomyosin cytoskeleton self-organization into arcs and stress f...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3466
更新日期：2017-02-01 00:00:00
abstract：:A number of key regulators of mouse embryonic stem (ES) cell identity, including the transcription factor Nanog, show strong expression fluctuations at the single-cell level. The molecular basis for these fluctuations is unknown. Here we used a genetic complementation strategy to investigate expression changes during ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2603
更新日期：2012-11-01 00:00:00
abstract：:Formation of a bipolar spindle is essential for faithful chromosome segregation at mitosis. Because centrosomes define spindle poles, defects in centrosome number and structural organization can lead to a loss of bipolarity. In addition, microtubule-mediated pulling and pushing forces acting on centrosomes and chromos...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1474
更新日期：2006-10-01 00:00:00
abstract：:Unrepaired DNA double-strand breaks (DSBs) cause genetic instability that leads to malignant transformation or cell death. Cells respond to DSBs with the ordered recruitment of signalling and repair proteins to the site of lesion. Protein modification with ubiquitin is crucial for the signalling cascade, but how ubiqu...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2367
更新日期：2011-10-23 00:00:00
