Abstract：
:Heterochromatin performs a central role in chromosome segregation and stability by promoting cohesion at centromeres. Establishment of both heterochromatin-mediated silencing and cohesion requires passage through S phase, although the mechanism is unknown. Here we demonstrate that Schizosaccharomyces pombe Hsk1 (CDC7), a conserved Dbf4-dependent protein kinase (DDK) that regulates replication initiation, interacts with and phosphorylates the heterochromatin protein 1 (HP1) equivalent Swi6 (ref. 6). Hsk1 and its regulatory subunit Dfp1 function downstream of Swi6 localization to promote heterochromatin function and cohesion specifically at centromeres. This role for Hsk1-Dfp1 is separable from its replication initiation activity, providing a temporal link between S phase and centromere cohesion that is mediated by heterochromatin.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Bailis JM,Bernard P,Antonelli R,Allshire RC,Forsburg SLdoi
10.1038/ncb1069keywords:
subject
Has Abstractpub_date
2003-12-01 00:00:00pages
1111-6issue
12eissn
1465-7392issn
1476-4679pii
ncb1069journal_volume
5pub_type
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