Abstract：
:During cell division, many animal cells transform into a spherical shape and assemble a contractile ring composed of actin filaments and myosin motors at the equator to separate the cell body into two. Although actomyosin regulatory proteins are spatio-temporally controlled during cytokinesis, the direct contribution of cell shape and actomyosin activity to the contractile ring assembly remains unclear. Here, we demonstrated in vitro that actin polymerization inside cell-sized spherical droplets induced the spontaneous formation of single ring-shaped actin bundles in the presence of bundling factors. Despite a lack of spatial regulatory signals, the rings always assembled at the equator to minimize the elastic energy of the bundles. Myosin promoted ring formation by the dynamic remodelling of actin networks, and an increase in the effective concentration of myosin triggered ring contraction. These results will help us understand how animal cells coordinate cell shape and actomyosin activities to direct cytokinesis.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Miyazaki M,Chiba M,Eguchi H,Ohki T,Ishiwata Sdoi
10.1038/ncb3142subject
Has Abstractpub_date
2015-04-01 00:00:00pages
480-9issue
4eissn
1465-7392issn
1476-4679pii
ncb3142journal_volume
17pub_type
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