Discrete interactions in cell adhesion measured by single-molecule force spectroscopy.

Abstract:

:Cell-cell adhesion mediated by specific cell-surface molecules is essential for multicellular development. Here we quantify de-adhesion forces at the resolution of individual cell-adhesion molecules, by controlling the interactions between single cells and combining single-molecule force spectroscopy with genetic manipulation. Our measurements are focused on a glycoprotein, contact site A (csA), as a prototype of cell-adhesion proteins. csA is expressed in aggregating cells of Dictyostelium discoideum, which are engaged in development of a multicellular organism. Adhesion between two adjacent cell surfaces involves discrete interactions characterized by an unbinding force of 23 +/- 8 pN, measured at a rupture rate of 2.5 +/- 0.5 microm s-1.

journal_name

Nat Cell Biol

journal_title

Nature cell biology

authors

Benoit M,Gabriel D,Gerisch G,Gaub HE

doi

10.1038/35014000

keywords:

subject

Has Abstract

pub_date

2000-06-01 00:00:00

pages

313-7

issue

6

eissn

1465-7392

issn

1476-4679

journal_volume

2

pub_type

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