Abstract:
:Cell-cell adhesion mediated by specific cell-surface molecules is essential for multicellular development. Here we quantify de-adhesion forces at the resolution of individual cell-adhesion molecules, by controlling the interactions between single cells and combining single-molecule force spectroscopy with genetic manipulation. Our measurements are focused on a glycoprotein, contact site A (csA), as a prototype of cell-adhesion proteins. csA is expressed in aggregating cells of Dictyostelium discoideum, which are engaged in development of a multicellular organism. Adhesion between two adjacent cell surfaces involves discrete interactions characterized by an unbinding force of 23 +/- 8 pN, measured at a rupture rate of 2.5 +/- 0.5 microm s-1.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Benoit M,Gabriel D,Gerisch G,Gaub HEdoi
10.1038/35014000keywords:
subject
Has Abstractpub_date
2000-06-01 00:00:00pages
313-7issue
6eissn
1465-7392issn
1476-4679journal_volume
2pub_type
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