Abstract:
:The actin cable is a supracellular structure that embryonic epithelia produce to close gaps. However, the action of the cable remains debated. Here, we address the function of the cable using Drosophila dorsal closure, a paradigm to understand wound healing. First, we show that the actin cytoskeleton protein Zasp52 is specifically required for actin cable formation. Next, we used Zasp52 loss of function to dissect the mechanism of action of the cable. Surprisingly, closure dynamics are perfect in Zasp52 mutants: the cable is therefore dispensable for closure, even in the absence of the amnioserosa. Conversely, we observed that the cable protects cellular geometries from robust morphogenetic forces that otherwise interfere with closure: the absence of cable results in defects in epithelial organization that lead to morphogenetic scarring. We propose that the cable prevents morphogenetic scarring by stabilizing cellular interactions rather than by acting on closure dynamics.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Ducuing A,Vincent Sdoi
10.1038/ncb3421subject
Has Abstractpub_date
2016-11-01 00:00:00pages
1149-1160issue
11eissn
1465-7392issn
1476-4679pii
ncb3421journal_volume
18pub_type
杂志文章abstract::Stereocilia are microvilli-derived mechanosensory organelles that are arranged in rows of graded heights on the apical surface of inner-ear hair cells. The 'staircase'-like architecture of stereocilia bundles is necessary to detect sound and head movement, and is achieved through differential elongation of the actin c...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1219
更新日期:2005-02-01 00:00:00
abstract::Topoisomerase II (Topo II) is required to separate intertwined sister chromatids before chromosome segregation can occur in mitosis. However, it remains to be resolved whether Topo II has any role in checkpoint control. Here we report that when phosphorylated, Ser 1524 of Topo IIalpha acts as a binding site for the BR...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1828
更新日期:2009-02-01 00:00:00
abstract::Pumilio proteins PUM1 and PUM2 are shown to regulate microRNA-dependent gene silencing by induction of a conformational switch in the 3' untranslated region of p27 mRNA. This conformational change is required for efficient microRNA-mediated repression of this cell-cycle regulator in rapidly proliferating cells. ...
journal_title:Nature cell biology
pub_type: 评论,新闻
doi:10.1038/ncb1010-928
更新日期:2010-10-01 00:00:00
abstract::Fetal haematopoietic stem cells (HSCs) self-renew extensively to build the blood system from scratch. The protein Lin28b negatively regulates the microRNA let-7 to keep levels of its target Hmga2 high, hence conferring high self-renewal potential to fetal HSCs. This regulatory circuit can be experimentally modulated t...
journal_title:Nature cell biology
pub_type: 评论,新闻
doi:10.1038/ncb2817
更新日期:2013-08-01 00:00:00
abstract::MicroRNAs (miRs) are a class of single-stranded, non-coding RNAs of about 22 nucleotides in length. Increasing evidence implicates miRs in myocardial disease processes. Here we show that miR-199b is a direct calcineurin/NFAT target gene that increases in expression in mouse and human heart failure, and targets the nuc...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2126
更新日期:2010-12-01 00:00:00
abstract::During meiosis, homologous chromosomes are sorted into pairs and are then intimately aligned, or synapsed, along their lengths while a proteinaceous structure, the synaptonemal complex, is assembled between them. However, little is known about how chromosomes first recognise each other. Here we show, by comparing the ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1168
更新日期:2004-09-01 00:00:00
abstract::The Drosophila fruitless (fru) gene product Fru has been postulated to be a neural sex-determination factor that directs the development of at least two male-specific characteristics, namely courtship behaviour and formation of the muscle of Lawrence (MOL). The fru gene encodes a putative transcription factor with a B...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35019537
更新日期:2000-08-01 00:00:00
abstract::Plant cell morphogenesis relies on the organization and function of two polymer arrays separated by the plasma membrane: the cortical microtubule cytoskeleton and cellulose microfibrils in the cell wall. Studies using in vivo markers confirmed that one function of the cortical microtubule array is to drive organizatio...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1886
更新日期:2009-07-01 00:00:00
abstract::Work over the past two years has led to a breakthrough in our understanding of the molecular basis of the directionality of the kinesin motor proteins. This breakthrough has come first from the reversal of directionality of the kinesin-related motor Ncd, followed closely by the reversal of kinesin's directionality and...
journal_title:Nature cell biology
pub_type: 杂志文章,评审
doi:10.1038/14113
更新日期:1999-10-01 00:00:00
abstract::Although it is well recognized that haematopoietic stem cells (HSCs) develop from a specialized population of endothelial cells known as haemogenic endothelium, the regulatory pathways that control this transition are not well defined. Here we identify Sox17 as a key regulator of haemogenic endothelial development. An...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2724
更新日期:2013-05-01 00:00:00
abstract::Humans suffer from high rates of fetal aneuploidy, often arising from the absence of meiotic crossover recombination between homologous chromosomes. Meiotic recombination is initiated by double-strand breaks (DSBs) generated by the SPO11 transesterase. In yeast and worms, at least one buffering mechanism, crossover ho...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2451
更新日期:2012-03-04 00:00:00
abstract::Protein degradation in eukaryotes often requires the ubiquitin-selective chaperone p97 for substrate recruitment and ubiquitin-chain assembly. However, the physiological relevance of p97, and its role in developmental processes, remain unclear. Here, we discover an unanticipated function for CDC-48/p97 in myosin assem...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1554
更新日期:2007-04-01 00:00:00
abstract::The Notch1 gene has an important role in mammalian cell-fate decision and tumorigenesis. Upstream control mechanisms for transcription of this gene are still poorly understood. In a chemical genetics screen for small molecule activators of Notch signalling, we identified epidermal growth factor receptor (EGFR) as a ke...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1750
更新日期:2008-08-01 00:00:00
abstract::The ATR checkpoint kinase coordinates cellular responses to DNA replication stress. Budding yeast contain three activators of Mec1 (the ATR orthologue); however, only TOPBP1 is known to activate ATR in vertebrates. We identified ETAA1 as a replication stress response protein in two proteomic screens. ETAA1-deficient c...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3415
更新日期:2016-11-01 00:00:00
abstract::In the unicellular eukaryote Saccharomyces cerevisiae, Cln3-cyclin-dependent kinase activity enables Start, the irreversible commitment to the cell division cycle. However, the concentration of Cln3 has been paradoxically considered to remain constant during G1, due to the presumed scaling of its production rate with ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/s41556-019-0413-3
更新日期:2019-11-01 00:00:00
abstract::Cohesin confers both intrachromatid and interchromatid cohesion through formation of a tripartite ring within which DNA is thought to be entrapped. Here, I discuss what is known about the four stages of the cohesin ring cycle using the ring model as an intellectual framework. I postulate that cohesin loading onto chro...
journal_title:Nature cell biology
pub_type: 杂志文章,评审
doi:10.1038/ncb2349
更新日期:2011-10-03 00:00:00
abstract::Actin dynamics is a tightly regulated process involved in various cellular events including biogenesis of clathrin-coated, AP-1 (adaptor protein 1)-coated transport carriers connecting the trans-Golgi network (TGN) and the endocytic pathway. However, the mechanisms coordinating coat assembly, membrane and actin remode...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2034
更新日期:2010-04-01 00:00:00
abstract::The mechanistic target of rapamycin (mTOR) functions as a critical regulator of cellular growth and metabolism by forming multi-component, yet functionally distinct complexes mTORC1 and mTORC2. Although mTORC2 has been implicated in mTORC1 activation, little is known about how mTORC2 is regulated. Here we report that ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2860
更新日期:2013-11-01 00:00:00
abstract::Junior researchers are encouraged to gain experience abroad, and for senior scientists, sabbaticals remain popular. France has taken the next step in fostering international exchange, by supporting long-term collaborations with foreign laboratories and by creating research units abroad. ...
journal_title:Nature cell biology
pub_type: 社论
doi:10.1038/ncb0607-613
更新日期:2007-06-01 00:00:00
abstract::Chromosome missegregation into a micronucleus can cause complex and localized genomic rearrangements known as chromothripsis, but the underlying mechanisms remain unresolved. Here we developed an inducible Y centromere-selective inactivation strategy by exploiting a CENP-A/histone H3 chimaera to directly examine the f...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3450
更新日期:2017-01-01 00:00:00
abstract:: ...
journal_title:Nature cell biology
pub_type: 评论,杂志文章
doi:10.1038/s41556-018-0246-5
更新日期:2018-12-01 00:00:00
abstract::The physical characteristics of tumours are intricately linked to the tumour phenotype and difficulties during treatment. Many factors contribute to the increased stiffness of tumours; from increased matrix deposition, matrix remodelling by forces from cancer cells and stromal fibroblasts, matrix crosslinking, increas...
journal_title:Nature cell biology
pub_type: 杂志文章,评审
doi:10.1038/s41556-018-0131-2
更新日期:2018-07-01 00:00:00
abstract::ES-cell-based cardiovascular repair requires an in-depth understanding of the molecular mechanisms underlying the differentiation of cardiovascular ES cells. A candidate cardiovascular-fate inducer is the bHLH transcription factor MesP1. As one of the earliest markers, it is expressed specifically in almost all cardio...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1696
更新日期:2008-03-01 00:00:00
abstract::Obesity occurs when excess energy accumulates in white adipose tissue (WAT), whereas brown adipose tissue (BAT), specialized for energy expenditure through thermogenesis, potently counteracts obesity. Factors that induce brown adipocyte commitment and energy expenditure would be a promising defence against adiposity. ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2867
更新日期:2013-12-01 00:00:00
abstract::For accurate segregation of chromosomes during cell division, microtubule fibres must attach sister kinetochores to opposite poles of the mitotic spindle (bi-orientation). Aurora kinases are linked to oncogenesis and have been implicated in the regulation of chromosome-microtubule attachments. Although loss of Aurora ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1102
更新日期:2004-03-01 00:00:00
abstract::Whereas total loss of Lis1 is lethal, disruption of one allele of the Lis1 gene results in brain abnormalities, indicating that developing neurons are particularly sensitive to a reduction in Lis1 dosage. Here we show that Lis1 is enriched in neurons relative to levels in other cell types, and that Lis1 interacts with...
journal_title:Nature cell biology
pub_type: 评论,杂志文章
doi:10.1038/35041000
更新日期:2000-11-01 00:00:00
abstract::During apoptosis, dying cells are swiftly removed by phagocytes. It is not fully understood how apoptotic cells are recognized by phagocytes. Here we report the identification and characterization of the Caenorhabditis elegans ttr-52 gene, which encodes a transthyretin-like protein and is required for efficient cell c...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2068
更新日期:2010-07-01 00:00:00
abstract::Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) involves a marked reorganization of chromatin. To identify post-translational histone modifications that change in global abundance during this process, we have applied a quantitative mass-spectrometry-based approach. We found that iPSCs, compa...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2768
更新日期:2013-07-01 00:00:00
abstract::Mitochondrial function degenerates with ageing and in ageing-related neuromuscular degenerative diseases, causing physiological decline of the cell. Factors that can delay the degenerative process are actively sought after. Here, we show that reduced cytosolic protein synthesis is a robust cellular strategy that suppr...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1769
更新日期:2008-09-01 00:00:00
abstract::Wnt/β-catenin signalling is an important regulator of liver development, zonation and regeneration. The cell surface complex RSPO-LGR4/5-ZNF3/RNF43 is now shown to direct Wnt/β-catenin signalling in orchestrating the division of the liver into functionally distinct metabolic zones, providing insights into the mechanis...
journal_title:Nature cell biology
pub_type: 评论,杂志文章
doi:10.1038/ncb3349
更新日期:2016-04-27 00:00:00