Abstract：
:Work over the past two years has led to a breakthrough in our understanding of the molecular basis of the directionality of the kinesin motor proteins. This breakthrough has come first from the reversal of directionality of the kinesin-related motor Ncd, followed closely by the reversal of kinesin's directionality and the finding that the Ncd 'neck' can convert Ncd or kinesin, which are intrinsically plus-end-directed microtubule motors, into a minus-end motor. These findings raise several outstanding questions, foremost, how does the neck function in motor directionality?
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Endow SAdoi
10.1038/14113keywords:
subject
Has Abstractpub_date
1999-10-01 00:00:00pages
E163-7issue
6eissn
1465-7392issn
1476-4679journal_volume
1pub_type
杂志文章,评审abstract：:During meiosis, homologous chromosomes are sorted into pairs and are then intimately aligned, or synapsed, along their lengths while a proteinaceous structure, the synaptonemal complex, is assembled between them. However, little is known about how chromosomes first recognise each other. Here we show, by comparing the ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1168
更新日期：2004-09-01 00:00:00
abstract：:Rad23 is a nucleotide-excision repair protein with a previously unknown biochemical function. We determined that yeast and human Rad23 inhibited multi-ubiquitin (Ub) chain formation and the degradation of proteolytic substrates. Significantly, Rad23 could be co-precipitated with a substrate that contained a short mult...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35023547
更新日期：2000-09-01 00:00:00
abstract：:While nuclear lamina abnormalities are hallmarks of human diseases, their interplay with epigenetic regulators and precise epigenetic landscape remain poorly understood. Here, we show that loss of the lysine acetyltransferase MOF or its associated NSL-complex members KANSL2 or KANSL3 leads to a stochastic accumulation...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-019-0397-z
更新日期：2019-10-01 00:00:00
abstract：:Given the important role of the hypothalamus in regulating feeding and metabolism, there has been considerable interest in a possible function for hypothalamic stem cells in modulating body weight in health and disease. Mice given a high-fat diet develop inflammation in the hypothalamus and lose key types of neurons. ...
journal_title：Nature cell biology
pub_type： 新闻
doi：10.1038/ncb2599
更新日期：2012-10-01 00:00:00
abstract：:Stereocilia are microvilli-derived mechanosensory organelles that are arranged in rows of graded heights on the apical surface of inner-ear hair cells. The 'staircase'-like architecture of stereocilia bundles is necessary to detect sound and head movement, and is achieved through differential elongation of the actin c...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1219
更新日期：2005-02-01 00:00:00
abstract：:Hippo signalling has been associated with many important tissue functions including the regulation of organ size. In the intestinal epithelium differing functions have been proposed for the effectors of Hippo signalling, YAP and TAZ1. These are now shown to have a dual role in the intestinal epithelium, regulating bot...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb3086
更新日期：2015-01-01 00:00:00
abstract：:The actin filament (F-actin) cytoskeleton associates dynamically with the plasma membrane and is thus ideally positioned to participate in endocytosis. Indeed, a wealth of genetic and biochemical evidence has confirmed that actin interacts with components of the endocytic machinery, although its precise function in en...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1025
更新日期：2003-08-01 00:00:00
abstract：:Canonical Wnt signalling has been implicated in mouse and human embryonic stem cell (ESC) maintenance; however, its requirement is controversial. β-catenin is the key component in this highly conserved Wnt pathway, acting as a transcriptional transactivator. However, β-catenin has additional roles at the plasma membra...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2260
更新日期：2011-06-19 00:00:00
abstract：:Protein ubiquitination regulates many cellular processes, including protein degradation, signal transduction, DNA repair and cell division. In the classical model, a uniform polyubiquitin chain that is linked through Lys 48 is required for recognition and degradation by the 26S proteasome. Here, we used a reconstitute...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1436
更新日期：2006-07-01 00:00:00
abstract：:Epigenetic mechanisms participate in the regulation of gene transcription in eukaryotes. Two studies in yeast have revealed an additional mechanism for controlling global gene transcription that is based on an inherited self-perpetuating change in the conformation of two different components of key transcriptional reg...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb0309-241
更新日期：2009-03-01 00:00:00
abstract：:Reprogrammed glucose metabolism as a result of increased glycolysis and glucose uptake is a hallmark of cancer. Here we show that cancer cells can suppress glucose uptake by non-tumour cells in the premetastatic niche, by secreting vesicles that carry high levels of the miR-122 microRNA. High miR-122 levels in the cir...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3094
更新日期：2015-02-01 00:00:00
abstract：:Although myosin II filaments are known to exist in non-muscle cells, their dynamics and organization are incompletely understood. Here, we combined structured illumination microscopy with pharmacological and genetic perturbations, to study the process of actomyosin cytoskeleton self-organization into arcs and stress f...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3466
更新日期：2017-02-01 00:00:00
abstract：:Formins have been implicated in the regulation of cytoskeletal structure in animals and fungi. Here we show that the formins Bni1 and Bnr1 of budding yeast stimulate the assembly of actin filaments that function as precursors to tropomyosin-stabilized cables that direct polarized cell growth. With loss of formin funct...
journal_title：Nature cell biology
pub_type： 更正并重新发布的文章,杂志文章
doi：10.1038/ncb770
更新日期：2002-03-01 00:00:00
abstract：:Treatment of cultured vertebrate neurons with nitric oxide leads to growth-cone collapse, axon retraction and the reconfiguration of axonal microtubules. We show that the light chain of microtubule-associated protein (MAP) 1B is a substrate for S-nitrosylation in vivo, in cultured cells and in vitro. S-nitrosylation o...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1625
更新日期：2007-09-01 00:00:00
abstract：:Individual neurons form specific elaborate dendritic structures that receive presynaptic information. The pattern of dendritic branching is regulated by the microtubule-associated motor protein dynein, which is responsible for the transport of essential endosomes and other organelles into the dendrites. ...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb1008-1131
更新日期：2008-10-01 00:00:00
abstract：:Primary cilia are required for vertebrate cells to respond to specific intercellular signals. Here we define when and where primary cilia appear in the mouse embryo using a transgenic line that expresses ARL13B-mCherry in cilia and Centrin 2-GFP in centrosomes. Primary cilia first appear on cells of the epiblast at E6...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3091
更新日期：2015-02-01 00:00:00
abstract：:Brown adipose tissue is a key metabolic organ that oxidizes fatty acids and glucose to generate heat. Through epigenomic analyses of multiple adipose depots, the transcription factor nuclear factor I-A (NFIA) is now shown to drive the brown fat genetic program through binding to lineage-specific cis-regulatory element...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3591
更新日期：2017-08-31 00:00:00
abstract：:As obligatory intracellular parasites, viruses rely on host-cell functions for most aspects of their replication cycle. This is born out during entry, when most viruses that infect vertebrate and insect cells exploit the endocytic activities of the host cell to move into the cytoplasm. Viruses belonging to vaccinia, a...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb0509-510
更新日期：2009-05-01 00:00:00
abstract：:Membrane association with mother centriole (M-centriole) distal appendages is critical for ciliogenesis initiation. How the Rab GTPase Rab11-Rab8 cascade functions in early ciliary membrane assembly is unknown. Here, we show that the membrane shaping proteins EHD1 and EHD3, in association with the Rab11-Rab8 cascade, ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3109
更新日期：2015-03-01 00:00:00
abstract：:Mammalian and most fungal infectious proteins (also known as prions) are self-propagating amyloid, a filamentous beta-sheet structure. A prion domain determines the infectious properties of a protein by forming the core of the amyloid. We compare the properties of known prion domains and their interactions with the re...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb1105-1039
更新日期：2005-11-01 00:00:00
abstract：:Research on genome stability and integrity now extends far beyond the biochemistry of DNA repair to encompass signal transduction pathways that span numerous aspects of cellular life. Derailed genomic integrity pathways can result in debilitating genetic disorders, premature ageing, predisposition to cancer and degene...
journal_title：Nature cell biology
pub_type：
doi：10.1038/ncb1004-923
更新日期：2004-10-01 00:00:00
abstract：:Stat3 has been shown to regulate lysosome membrane permeabilization and cell death in vivo during post-lactation mammary gland involution. It is now revealed that Stat3 induces lysosome membrane permeabilization by causing phagocytosis of milk fat globules, which destabilize the lysosome membrane leading to leakage of...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb3059
更新日期：2014-11-01 00:00:00
abstract：:We describe a genome-wide gain-of-function screen for regulators of NF-kappaB, and identify Rap1 (Trf2IP), as an essential modulator of NF-kappaB-mediated pathways. NF-kappaB is induced by ectopic expression of Rap1, whereas its activity is inhibited by Rap1 depletion. In addition to localizing on telomeres, mammalian...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2080
更新日期：2010-08-01 00:00:00
abstract：:The rising incidence of obesity and related disorders such as diabetes and heart disease has focused considerable attention on the discovery of new therapeutics. One promising approach has been to increase the number or activity of brown-like adipocytes in white adipose depots, as this has been shown to prevent diet-i...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3075
更新日期：2015-01-01 00:00:00
abstract：:Tumour growth and invasiveness require extracellular matrix (ECM) degradation and are stimulated by the GALA pathway, which induces protein O-glycosylation in the endoplasmic reticulum (ER). ECM degradation requires metalloproteases, but whether other enzymes are required is unclear. Here, we show that GALA induces th...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-020-00590-w
更新日期：2020-11-01 00:00:00
abstract：:Clathrin-mediated endocytosis is independent of actin dynamics in many circumstances but requires actin polymerization in others. We show that membrane tension determines the actin dependence of clathrin-coat assembly. As found previously, clathrin assembly supports formation of mature coated pits in the absence of ac...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2307
更新日期：2011-08-14 00:00:00
abstract：:Telomere attrition and other forms of telomere damage can activate the ATM kinase pathway. What generates the DNA damage signal at mammalian chromosome ends or at other double-strand breaks is not known. Telomere dysfunction is often accompanied by disappearance of the 3' telomeric overhang, raising the possibility th...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1275
更新日期：2005-07-01 00:00:00
abstract：:Inactivation of the von Hippel-Lindau (VHL) tumour-suppressor protein (pVHL) is associated with the von Hippel-Lindau cancer syndrome and the majority of kidney cancers. New evidence suggests that pVHL has properties of an F-box protein that targets the alpha-subunits of hypoxia-inducible factor (HIF)-1 for oxygen-dep...
journal_title：Nature cell biology
pub_type： 评论,新闻
doi：10.1038/35017129
更新日期：2000-07-01 00:00:00
abstract：:Examination of the tumour suppressor adenomatous polyposis coli (APC) has shown that it may be multifunctional. Recent work has demonstrated dynamic interactions of APC with the microtubule cytoskeleton, supporting the idea that APC has an important function in cell migration. ...
journal_title：Nature cell biology
pub_type： 新闻,评审
doi：10.1038/35008685
更新日期：2000-04-01 00:00:00
abstract：:Phosphatase and tensin homologue (Pten) suppresses neoplastic growth by negatively regulating PI(3)K signalling through its phosphatase activity. To gain insight into the actions of non-catalytic Pten domains in normal physiological processes and tumorigenesis, we engineered mice lacking the PDZ-binding domain (PDZ-BD...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3369
更新日期：2016-07-01 00:00:00
