Abstract:
:Clathrin-mediated endocytosis is independent of actin dynamics in many circumstances but requires actin polymerization in others. We show that membrane tension determines the actin dependence of clathrin-coat assembly. As found previously, clathrin assembly supports formation of mature coated pits in the absence of actin polymerization on both dorsal and ventral surfaces of non-polarized mammalian cells, and also on basolateral surfaces of polarized cells. Actin engagement is necessary, however, to complete membrane deformation into a coated pit on apical surfaces of polarized cells and, more generally, on the surface of any cell in which the plasma membrane is under tension from osmotic swelling or mechanical stretching. We use these observations to alter actin dependence experimentally and show that resistance of the membrane to propagation of the clathrin lattice determines the distinction between 'actin dependent and 'actin independent'. We also find that light-chain-bound Hip1R mediates actin engagement. These data thus provide a unifying explanation for the role of actin dynamics in coated-pit budding.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Boulant S,Kural C,Zeeh JC,Ubelmann F,Kirchhausen Tdoi
10.1038/ncb2307subject
Has Abstractpub_date
2011-08-14 00:00:00pages
1124-31issue
9eissn
1465-7392issn
1476-4679pii
ncb2307journal_volume
13pub_type
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