Abstract：
:Stereocilia are microvilli-derived mechanosensory organelles that are arranged in rows of graded heights on the apical surface of inner-ear hair cells. The 'staircase'-like architecture of stereocilia bundles is necessary to detect sound and head movement, and is achieved through differential elongation of the actin core of each stereocilium to a predetermined length. Abnormally short stereocilia bundles that have a diminished staircase are characteristic of the shaker 2 (Myo15a(sh2)) and whirler (Whrn(wi)) strains of deaf mice. We show that myosin-XVa is a motor protein that, in vivo, interacts with the third PDZ domain of whirlin through its carboxy-terminal PDZ-ligand. Myosin-XVa then delivers whirlin to the tips of stereocilia. Moreover, if green fluorescent protein (GFP)-Myo15a is transfected into hair cells of Myo15a(sh2) mice, the wild-type pattern of hair bundles is restored by recruitment of endogenous whirlin to the tips of stereocilia. The interaction of myosin-XVa and whirlin is therefore a key event in hair-bundle morphogenesis.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Belyantseva IA,Boger ET,Naz S,Frolenkov GI,Sellers JR,Ahmed ZM,Griffith AJ,Friedman TBdoi
10.1038/ncb1219keywords:
subject
Has Abstractpub_date
2005-02-01 00:00:00pages
148-56issue
2eissn
1465-7392issn
1476-4679pii
ncb1219journal_volume
7pub_type
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