Abstract：
:The importance of herpes simplex viruses (HSV) as human pathogens and the emerging prospect of using mutant derivatives of HSV-1 as potential anti-cancer therapeutics have necessitated a thorough investigation into the molecular basis of host-cell permissiveness to HSV. Here we show that NIH-3T3 cells transformed with the oncogenes v-erbB, activated sos or activated ras become significantly more permissive to HSV-1. Inhibitors of the Ras signalling pathway, such as farnesyl transferase inhibitor 1 and PD98059, effectively suppressed HSV-1 infection of ras-transformed cells. Enhanced permissiveness of the transformed cells was linked to the inhibition of virus-induced activation (phosphorylation) of the double-stranded RNA-activated protein kinase (PKR), thereby allowing viral transcripts to be translated in these cells. An HSV-1-derived oncolytic mutant, R3616, was also found to infect preferentially both transformed cells and PKR-/- (but not PKR+/+) mouse embryo fibroblasts. These observations suggest that HSV-1 specifically targets cells with an activated Ras signalling pathway, and have important ramifications in the use of engineered HSV in cancer therapy, the development of strategies against HSV infections, and the controversial role of HSV in human cancers.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Farassati F,Yang AD,Lee PWdoi
10.1038/35087061keywords:
subject
Has Abstractpub_date
2001-08-01 00:00:00pages
745-50issue
8eissn
1465-7392issn
1476-4679pii
35087061journal_volume
3pub_type
杂志文章abstract：:Individual neurons form specific elaborate dendritic structures that receive presynaptic information. The pattern of dendritic branching is regulated by the microtubule-associated motor protein dynein, which is responsible for the transport of essential endosomes and other organelles into the dendrites. ...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb1008-1131
更新日期：2008-10-01 00:00:00
abstract：:Fetal haematopoietic stem cells (HSCs) self-renew extensively to build the blood system from scratch. The protein Lin28b negatively regulates the microRNA let-7 to keep levels of its target Hmga2 high, hence conferring high self-renewal potential to fetal HSCs. This regulatory circuit can be experimentally modulated t...
journal_title：Nature cell biology
pub_type： 评论,新闻
doi：10.1038/ncb2817
更新日期：2013-08-01 00:00:00
abstract：:Reference datasets should be accessible independently of scientific papers in a citable form, allowing attribution. ...
journal_title：Nature cell biology
pub_type： 社论
doi：10.1038/ncb1109-1273a
更新日期：2009-11-01 00:00:00
abstract：:When brassinosteroid levels are low, the GSK3-like kinase BIN2 phosphorylates and inactivates the BZR1 transcription factor to inhibit growth in plants. Brassinosteroid promotes growth by inducing dephosphorylation of BZR1, but the phosphatase that dephosphorylates BZR1 has remained unknown. Here, using tandem affinit...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2151
更新日期：2011-02-01 00:00:00
abstract：:Unrepaired DNA double-strand breaks (DSBs) cause genetic instability that leads to malignant transformation or cell death. Cells respond to DSBs with the ordered recruitment of signalling and repair proteins to the site of lesion. Protein modification with ubiquitin is crucial for the signalling cascade, but how ubiqu...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2367
更新日期：2011-10-23 00:00:00
abstract：:Genomic integrity is maintained by checkpoints that guard against undesired replication after DNA damage. Here, we show that CDT1, a licensing factor of the pre-replication complex (preRC), is rapidly proteolysed after UV- or gamma-irradiation. The preRC assembles on replication origins at the end of mitosis and durin...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1061
更新日期：2003-11-01 00:00:00
abstract：:In the version of this Article originally published, ref. 52 was incorrectly only attributed to its corresponding author, Fre, S., and an older title was used. The correct citation should have been: Lilja, A. M. et al. Clonal analysis of Notch1-expressing cells reveals the existence of unipotent stem cells that retain...
journal_title：Nature cell biology
pub_type： 已发布勘误
doi：10.1038/s41556-018-0164-6
更新日期：2018-09-01 00:00:00
abstract：: ...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/s41556-018-0224-y
更新日期：2018-11-01 00:00:00
abstract：:The mechanistic target of rapamycin (mTOR) functions as a critical regulator of cellular growth and metabolism by forming multi-component, yet functionally distinct complexes mTORC1 and mTORC2. Although mTORC2 has been implicated in mTORC1 activation, little is known about how mTORC2 is regulated. Here we report that ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2860
更新日期：2013-11-01 00:00:00
abstract：:The cytokine tumour necrosis factor (TNF) and the toll-like receptors (TLRs) coordinate immune responses by activating inflammatory transcriptional programs, but these signals can also trigger cell death. Recent studies identify the MAP kinase substrate MK2 as a key player in determining whether cells live or die in r...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3619
更新日期：2017-09-29 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1314
更新日期：2005-11-01 00:00:00
abstract：:To control cell proliferation, signal transduction needs to regulate the cell-cycle machinery. Recent findings show that Akt - a major kinase that coordinates diverse signalling pathways - phosphorylates Skp2, a subunit of the SCF-Skp2 ubiquitin ligase that targets key cell-cycle regulators. Akt1-dependent phosphoryla...
journal_title：Nature cell biology
pub_type： 评论,新闻
doi：10.1038/ncb0409-377
更新日期：2009-04-01 00:00:00
abstract：:Epithelial-mesenchymal transition (EMT) is essential for organogenesis and is triggered during carcinoma progression to an invasive state. Transforming growth factor-beta (TGF-beta) cooperates with signalling pathways, such as Ras and Wnt, to induce EMT, but the molecular mechanisms are not clear. Here, we report that...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1905
更新日期：2009-08-01 00:00:00
abstract：:Cellular senescence is a tumour suppressor programme characterized by a stable cell cycle arrest. Here we report that cellular senescence triggered by a variety of stimuli leads to diminished ribosome biogenesis and the accumulation of both rRNA precursors and ribosomal proteins. These defects were associated with red...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-018-0127-y
更新日期：2018-07-01 00:00:00
abstract：:The guanosine tri-phosphatase Ran stimulates assembly of microtubule spindles. However, it is not known what aspects of the microtubule cytoskeleton are subject to regulation by Ran in mitosis. Here we show that Ran-GTP stimulates microtubule assembly by increasing the rescue frequency of microtubules three- to eightf...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35060000
更新日期：2001-03-01 00:00:00
abstract：:Loss of spindle-pole integrity during mitosis leads to multipolarity independent of centrosome amplification. Multipolar-spindle conformation favours incorrect kinetochore-microtubule attachments, compromising faithful chromosome segregation and daughter-cell viability. Spindle-pole organization influences and is infl...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2423
更新日期：2012-02-05 00:00:00
abstract：:Cancer-induced immune responses affect tumour progression and therapeutic response. In multiple murine models and clinical datasets, we identified large variations of neutrophils and macrophages that define 'immune subtypes' of triple-negative breast cancer (TNBC), including neutrophil-enriched (NES) and macrophage-en...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-019-0373-7
更新日期：2019-09-01 00:00:00
abstract：:FoxG1 is an evolutionarily conserved, winged-helix transcriptional repressor that maintains progenitor cells in the vertebrate forebrain. How the activity of FoxG1 is regulated is not known. Here, we report that in the developing Xenopus and mouse forebrain, FoxG1 is nuclear in progenitor cells but cytoplasmic in diff...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1573
更新日期：2007-05-01 00:00:00
abstract：:Influx of Ca(2+) through store-operated Ca(2+) channels (SOCs) is a central component of receptor-evoked Ca(2+) signals. Orai channels are SOCs that are gated by STIM1, a Ca(2+) sensor located in the ER but how it gates and regulates the Orai channels is unknown. Here, we report the molecular basis for gating of Orais...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1842
更新日期：2009-03-01 00:00:00
abstract：:The Notch1 gene has an important role in mammalian cell-fate decision and tumorigenesis. Upstream control mechanisms for transcription of this gene are still poorly understood. In a chemical genetics screen for small molecule activators of Notch signalling, we identified epidermal growth factor receptor (EGFR) as a ke...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1750
更新日期：2008-08-01 00:00:00
abstract：:Tau is an abundant microtubule-associated protein in neurons. Tau aggregation into insoluble fibrils is a hallmark of Alzheimer's disease and other types of dementia1, yet the physiological state of tau molecules within cells remains unclear. Using single-molecule imaging, we directly observe that the microtubule latt...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-019-0375-5
更新日期：2019-09-01 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb0901-e201
更新日期：2001-09-01 00:00:00
abstract：:The migration of border cells during Drosophila melanogaster oogenesis is a simple and powerful system for studying invasive cell migration in vivo. Border cells are somatic cells that delaminate from the follicular epithelium of an egg chamber and invade the germ line cluster. They migrate between the nurse cells to ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb848
更新日期：2002-09-01 00:00:00
abstract：:The molecular mechanisms underlying the formation of carriers trafficking from the Golgi complex to the cell surface are still ill-defined; nevertheless, the involvement of a lipid-based machinery is well established. This includes phosphatidylinositol 4-phosphate (PtdIns(4)P), the precursor for phosphatidylinositol 4...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1119
更新日期：2004-05-01 00:00:00
abstract：:Phosphatase and tensin homologue (Pten) suppresses neoplastic growth by negatively regulating PI(3)K signalling through its phosphatase activity. To gain insight into the actions of non-catalytic Pten domains in normal physiological processes and tumorigenesis, we engineered mice lacking the PDZ-binding domain (PDZ-BD...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3369
更新日期：2016-07-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature cell biology
pub_type： 已发布勘误
doi：10.1038/s41556-019-0389-z
更新日期：2019-10-01 00:00:00
abstract：:During meiosis, homologous chromosomes are sorted into pairs and are then intimately aligned, or synapsed, along their lengths while a proteinaceous structure, the synaptonemal complex, is assembled between them. However, little is known about how chromosomes first recognise each other. Here we show, by comparing the ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1168
更新日期：2004-09-01 00:00:00
abstract：:During apoptosis, dying cells are swiftly removed by phagocytes. It is not fully understood how apoptotic cells are recognized by phagocytes. Here we report the identification and characterization of the Caenorhabditis elegans ttr-52 gene, which encodes a transthyretin-like protein and is required for efficient cell c...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2068
更新日期：2010-07-01 00:00:00
abstract：:Focal adhesions are integrin-based multiprotein complexes, several micrometres in diameter, that mechanically link the extracellular matrix with the termini of actin bundles. The molecular diversity of focal adhesions and their role in cell migration and matrix sensing has been extensively studied, but their ultrastru...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2095
更新日期：2010-09-01 00:00:00
abstract：:In chromaffin cells, exocytosis of single granules and properties of the fusion pore--the first connection between vesicular lumen and extracellular space --can be studied by cell-attached patch amperometry, which couples patch-clamp capacitance measurements with simultaneous amperometric recordings of transmitter rel...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb956
更新日期：2003-04-01 00:00:00
