Abstract:
:Fetal haematopoietic stem cells (HSCs) self-renew extensively to build the blood system from scratch. The protein Lin28b negatively regulates the microRNA let-7 to keep levels of its target Hmga2 high, hence conferring high self-renewal potential to fetal HSCs. This regulatory circuit can be experimentally modulated to boost the lower self-renewal activity of quiescent adult HSCs.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Pietras EM,Passegué Edoi
10.1038/ncb2817subject
Has Abstractpub_date
2013-08-01 00:00:00pages
885-7issue
8eissn
1465-7392issn
1476-4679pii
ncb2817journal_volume
15pub_type
评论,新闻abstract::Protein ubiquitination regulates many cellular processes, including protein degradation, signal transduction, DNA repair and cell division. In the classical model, a uniform polyubiquitin chain that is linked through Lys 48 is required for recognition and degradation by the 26S proteasome. Here, we used a reconstitute...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1436
更新日期:2006-07-01 00:00:00
abstract::Although nuclear envelope (NE) assembly is known to require the GTPase Ran, the membrane fusion machinery involved is uncharacterized. NE assembly involves formation of a reticular network on chromatin, fusion of this network into a closed NE and subsequent expansion. Here we show that p97, an AAA-ATPase previously im...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1201-1086
更新日期:2001-12-01 00:00:00
abstract::In mammals, motile cilia cover many organs, such as fallopian tubes, respiratory tracts and brain ventricles. The development and function of these organs critically depend on efficient directional fluid flow ensured by the alignment of ciliary beating. To identify the mechanisms involved in this process, we analysed ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2040
更新日期:2010-04-01 00:00:00
abstract::Whereas total loss of Lis1 is lethal, disruption of one allele of the Lis1 gene results in brain abnormalities, indicating that developing neurons are particularly sensitive to a reduction in Lis1 dosage. Here we show that Lis1 is enriched in neurons relative to levels in other cell types, and that Lis1 interacts with...
journal_title:Nature cell biology
pub_type: 评论,杂志文章
doi:10.1038/35041000
更新日期:2000-11-01 00:00:00
abstract::The contribution of autophagy to cancer development remains controversial, largely owing to the fact that autophagy can be tumour suppressive or oncogenic in different biological contexts. Here, we show that in non-small-cell lung cancer (NSCLC), casein kinase 1 alpha 1 (CK1α) suppresses tumour growth by functioning a...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/s41556-018-0065-8
更新日期:2018-04-01 00:00:00
abstract::The serine/threonine protein kinase Akt is involved in a variety of cellular processes including cell proliferation, survival, metabolism and gene expression. It is essential in vascular endothelial growth factor (VEGF)-mediated angiogenesis; however, it is not known how Akt regulates the migration of endothelial cell...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1695
更新日期:2008-03-01 00:00:00
abstract::MicroRNAs (miRNAs) have emerged as novel cancer genes. In particular, the miR-17-92 cluster, containing six individual miRNAs, is highly expressed in haematopoietic cancers and promotes lymphomagenesis in vivo. Clinical use of these findings hinges on isolating the oncogenic activity within the 17-92 cluster and defin...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2037
更新日期:2010-04-01 00:00:00
abstract::Treatment of cultured vertebrate neurons with nitric oxide leads to growth-cone collapse, axon retraction and the reconfiguration of axonal microtubules. We show that the light chain of microtubule-associated protein (MAP) 1B is a substrate for S-nitrosylation in vivo, in cultured cells and in vitro. S-nitrosylation o...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1625
更新日期:2007-09-01 00:00:00
abstract::Structural maintenance of chromosomes (SMC) protein complexes are key determinants of chromosome conformation. Using Hi-C and polymer modelling, we study how cohesin and condensin, two deeply conserved SMC complexes, organize chromosomes in the budding yeast Saccharomyces cerevisiae. The canonical role of cohesin is t...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3594
更新日期:2017-09-01 00:00:00
abstract::The cyclical protrusion and retraction of the leading edge is a hallmark of many migrating cells involved in processes such as development, inflammation and tumorigenesis. The molecular identity of the signalling mechanisms that control these cycles has remained unknown. Here, we used live-cell imaging of biosensors t...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2231
更新日期:2011-06-01 00:00:00
abstract::The promyelocytic leukaemia (PML) tumour-suppressor protein potentiates p53 function by regulating post-translational modifications, such as CBP-dependent acetylation and Chk2-dependent phosphorylation, in the PML-Nuclear Body (NB). PML was recently shown to interact with the p53 ubiquitin-ligase Mdm2 (refs 4-6); howe...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1147
更新日期:2004-07-01 00:00:00
abstract::It is now well established that stromal interaction molecule 1 (STIM1) is the calcium sensor of endoplasmic reticulum stores required to activate store-operated calcium entry (SOC) channels at the surface of non-excitable cells. However, little is known about STIM1 in excitable cells, such as striated muscle, where th...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1731
更新日期:2008-06-01 00:00:00
abstract::Symmetrical cell division requires duplication of DNA and protein content to generate two daughter cells. Centrosomes also duplicate during cell division, but the mechanism controlling this process is incompletely understood. We describe an alternative splice form of SadB encoding a short SADB Ser/Thr kinase whose act...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1921
更新日期:2009-09-01 00:00:00
abstract::Mammalian telomeres consist of non-coding TTAGGG repeats that are bound by the multi-protein complex 'shelterin', thus protecting chromosome ends from DNA repair mechanisms and degradation. Mammalian telomeric chromatin is enriched for the constitutive heterochromatin marks H3K9me3, H4K20me3 and HP1 (refs 2, 3, 4, 5, ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1685
更新日期:2008-02-01 00:00:00
abstract::The actin cable is a supracellular structure that embryonic epithelia produce to close gaps. However, the action of the cable remains debated. Here, we address the function of the cable using Drosophila dorsal closure, a paradigm to understand wound healing. First, we show that the actin cytoskeleton protein Zasp52 is...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3421
更新日期:2016-11-01 00:00:00
abstract::Recent theoretical analysis of a model lattice of interacting transmembrane receptor proteins has indicated that such clustering in the membrane could provide a novel mechanism for regulating receptor signalling in cells. It has been calculated that cooperative interactions between receptors organized into a cluster, ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35023625
更新日期:2000-09-01 00:00:00
abstract::The target of rapamycin complex 2 (TORC2) plays a key role in maintaining the homeostasis of plasma membrane (PM) tension. TORC2 activation following increased PM tension involves redistribution of the Slm1 and 2 paralogues from PM invaginations known as eisosomes into membrane compartments containing TORC2. How Slm1/...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/s41556-018-0150-z
更新日期:2018-09-01 00:00:00
abstract::Formation of a bipolar spindle is essential for faithful chromosome segregation at mitosis. Because centrosomes define spindle poles, defects in centrosome number and structural organization can lead to a loss of bipolarity. In addition, microtubule-mediated pulling and pushing forces acting on centrosomes and chromos...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1474
更新日期:2006-10-01 00:00:00
abstract::In the version of this Article originally published, ref. 52 was incorrectly only attributed to its corresponding author, Fre, S., and an older title was used. The correct citation should have been: Lilja, A. M. et al. Clonal analysis of Notch1-expressing cells reveals the existence of unipotent stem cells that retain...
journal_title:Nature cell biology
pub_type: 已发布勘误
doi:10.1038/s41556-018-0164-6
更新日期:2018-09-01 00:00:00
abstract::Centromeres are specialized chromosomal domains that direct kinetochore assembly during mitosis. CENP-A (centromere protein A), a histone H3-variant present exclusively in centromeric nucleosomes, is thought to function as an epigenetic mark that specifies centromere identity. Here we identify the essential centromere...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1899
更新日期:2009-07-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature cell biology
pub_type: 已发布勘误
doi:10.1038/s41556-019-0389-z
更新日期:2019-10-01 00:00:00
abstract::Microtubules are central elements of the eukaryotic cytoskeleton that often function as part of branched networks. Current models for branching include nucleation of new microtubules from severed microtubule seeds or from γ-tubulin recruited to the side of a pre-existing microtubule. Here, we found that microtubules c...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/s41556-018-0199-8
更新日期:2018-10-01 00:00:00
abstract::Bcl-2 stimulates mutagenesis after the exposure of cells to DNA-damaging agents. However, the biological mechanisms of Bcl-2-mediated mutagenesis have remained largely obscure. Here we demonstrate that the Bcl-2-mediated suppression of hMSH2 expression results in a reduced cellular capacity to repair mismatches. The p...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1215
更新日期:2005-02-01 00:00:00
abstract::RNA interference has become an indispensable tool for loss-of-function studies across eukaryotes. By enabling stable and reversible gene silencing, shRNAs provide a means to study long-term phenotypes, perform pool-based forward genetic screens and examine the consequences of temporary target inhibition in vivo. Howev...
journal_title:Nature cell biology
pub_type: 杂志文章,评审
doi:10.1038/ncb2895
更新日期:2014-01-01 00:00:00
abstract::In the version of this Article originally published, the author had misnumbered the reference citations in the Methods, using numbers 1-14 instead of 46-59. These errors have now been corrected in all online versions of the Article. ...
journal_title:Nature cell biology
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41556-018-0053-z
更新日期:2018-08-01 00:00:00
abstract::Apoptosis is induced by caspases, which are members of the cysteine protease family. Caspases are synthesized as inactive zymogens and initiator caspases first gain activity by associating with an oligomeric complex of their adaptor proteins, such as the apoptosome. Activated initiator caspases subsequently cleave and...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1803
更新日期:2008-12-01 00:00:00
abstract::Endothelial cells and leptin receptor+ (LepR+) stromal cells are critical sources of haematopoietic stem cell (HSC) niche factors, including stem cell factor (SCF), in bone marrow. After irradiation or chemotherapy, these cells are depleted while adipocytes become abundant. We discovered that bone marrow adipocytes sy...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3570
更新日期:2017-08-01 00:00:00
abstract::Coordination of microtubules and the actin cytoskeleton is important in several types of cell movement. mDia1 is a member of the formin-homology family of proteins and an effector of the small GTPase Rho. It contains the Rho-binding domain in its amino terminus and two distinct regions of formin homology, FH1 in the m...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35050598
更新日期:2001-01-01 00:00:00
abstract::During the development of multicellular organisms, concerted actions of molecular signalling networks determine whether cells undergo proliferation, differentiation, death or ageing. Here we show that genetic inactivation of the stress signalling kinase, MKK7, a direct activator of JNKs in mice, results in embryonic l...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1098
更新日期:2004-03-01 00:00:00
abstract::To control cell proliferation, signal transduction needs to regulate the cell-cycle machinery. Recent findings show that Akt - a major kinase that coordinates diverse signalling pathways - phosphorylates Skp2, a subunit of the SCF-Skp2 ubiquitin ligase that targets key cell-cycle regulators. Akt1-dependent phosphoryla...
journal_title:Nature cell biology
pub_type: 评论,新闻
doi:10.1038/ncb0409-377
更新日期:2009-04-01 00:00:00