Abstract:
:The rising incidence of obesity and related disorders such as diabetes and heart disease has focused considerable attention on the discovery of new therapeutics. One promising approach has been to increase the number or activity of brown-like adipocytes in white adipose depots, as this has been shown to prevent diet-induced obesity and reduce the incidence and severity of type 2 diabetes. Thus, the conversion of fat-storing cells into metabolically active thermogenic cells has become an appealing therapeutic strategy to combat obesity. Here, we report a screening platform for the identification of small molecules capable of promoting a white-to-brown metabolic conversion in human adipocytes. We identified two inhibitors of Janus kinase (JAK) activity with no precedent in adipose tissue biology that stably confer brown-like metabolic activity to white adipocytes. Importantly, these metabolically converted adipocytes exhibit elevated UCP1 expression and increased mitochondrial activity. We further found that repression of interferon signalling and activation of hedgehog signalling in JAK-inactivated adipocytes contributes to the metabolic conversion observed in these cells. Our findings highlight a previously unknown role for the JAK-STAT pathway in the control of adipocyte function and establish a platform to identify compounds for the treatment of obesity.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Moisan A,Lee YK,Zhang JD,Hudak CS,Meyer CA,Prummer M,Zoffmann S,Truong HH,Ebeling M,Kiialainen A,Gérard R,Xia F,Schinzel RT,Amrein KE,Cowan CAdoi
10.1038/ncb3075subject
Has Abstractpub_date
2015-01-01 00:00:00pages
57-67issue
1eissn
1465-7392issn
1476-4679pii
ncb3075journal_volume
17pub_type
杂志文章abstract::The stability and membrane localization of the transforming growth factor-β (TGF-β) type I receptor (TβRI) determines the levels of TGF-β signalling. TβRI is targeted for ubiquitylation-mediated degradation by the SMAD7-SMURF2 complex. Here we performed a genome-wide gain-of-function screen and identified ubiquitin-sp...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2522
更新日期:2012-06-17 00:00:00
abstract::The mechanistic target of rapamycin (mTOR) functions as a critical regulator of cellular growth and metabolism by forming multi-component, yet functionally distinct complexes mTORC1 and mTORC2. Although mTORC2 has been implicated in mTORC1 activation, little is known about how mTORC2 is regulated. Here we report that ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2860
更新日期:2013-11-01 00:00:00
abstract::Examination of the tumour suppressor adenomatous polyposis coli (APC) has shown that it may be multifunctional. Recent work has demonstrated dynamic interactions of APC with the microtubule cytoskeleton, supporting the idea that APC has an important function in cell migration. ...
journal_title:Nature cell biology
pub_type: 新闻,评审
doi:10.1038/35008685
更新日期:2000-04-01 00:00:00
abstract::Vaccinia virus, a close relative of the causative agent of smallpox, exploits actin polymerization to enhance its cell-to-cell spread. We show that actin-based motility of vaccinia is initiated only at the plasma membrane and remains associated with it. There must therefore be another form of cytoplasmic viral transpo...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1101-992
更新日期:2001-11-01 00:00:00
abstract::Phosphoinositides (PIs) undergo phosphorylation/dephosphorylation cycles through organelle-specific PI kinases and PI phosphatases that lead to distinct subcellular distributions of the individual PI species. Specific PIs control the correct timing and location of many trafficking events. Their ultimate mode of action...
journal_title:Nature cell biology
pub_type: 杂志文章,评审
doi:10.1038/ncb0604-487
更新日期:2004-06-01 00:00:00
abstract::Epigenetic mechanisms participate in the regulation of gene transcription in eukaryotes. Two studies in yeast have revealed an additional mechanism for controlling global gene transcription that is based on an inherited self-perpetuating change in the conformation of two different components of key transcriptional reg...
journal_title:Nature cell biology
pub_type: 评论,杂志文章
doi:10.1038/ncb0309-241
更新日期:2009-03-01 00:00:00
abstract::Cancer cells use aerobic glycolysis preferentially for energy provision and this metabolic change is important for tumour growth. Here, we have found a link between the tumour suppressor p53, the transcription factor NF-kappaB and glycolysis. In p53-deficient primary cultured cells, kinase activities of IKKalpha and I...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1724
更新日期:2008-05-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) are generated via the expression of the transcription factors OCT4 (also known as POU5F1), SOX2, KLF4 and cMYC (OSKM) in somatic cells. In contrast to murine naive iPSCs, conventional human iPSCs are in a more developmentally advanced state called primed pluripotency. Here, we re...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/s41556-018-0254-5
更新日期:2019-02-01 00:00:00
abstract::Canonical Wnt signalling has been implicated in mouse and human embryonic stem cell (ESC) maintenance; however, its requirement is controversial. β-catenin is the key component in this highly conserved Wnt pathway, acting as a transcriptional transactivator. However, β-catenin has additional roles at the plasma membra...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2260
更新日期:2011-06-19 00:00:00
abstract::The Notch1 gene has an important role in mammalian cell-fate decision and tumorigenesis. Upstream control mechanisms for transcription of this gene are still poorly understood. In a chemical genetics screen for small molecule activators of Notch signalling, we identified epidermal growth factor receptor (EGFR) as a ke...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1750
更新日期:2008-08-01 00:00:00
abstract::Chromosome missegregation into a micronucleus can cause complex and localized genomic rearrangements known as chromothripsis, but the underlying mechanisms remain unresolved. Here we developed an inducible Y centromere-selective inactivation strategy by exploiting a CENP-A/histone H3 chimaera to directly examine the f...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3450
更新日期:2017-01-01 00:00:00
abstract::When brassinosteroid levels are low, the GSK3-like kinase BIN2 phosphorylates and inactivates the BZR1 transcription factor to inhibit growth in plants. Brassinosteroid promotes growth by inducing dephosphorylation of BZR1, but the phosphatase that dephosphorylates BZR1 has remained unknown. Here, using tandem affinit...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2151
更新日期:2011-02-01 00:00:00
abstract::PX domains are found in a variety of proteins that associate with cell membranes, but their molecular function has remained obscure. We show here that the PX domains in p47phox and p40phox subunits of the phagocyte NADPH oxidase bind to phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4)P(2)) and phosphatidylinositol-3...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35083070
更新日期:2001-07-01 00:00:00
abstract::Tumour growth and invasiveness require extracellular matrix (ECM) degradation and are stimulated by the GALA pathway, which induces protein O-glycosylation in the endoplasmic reticulum (ER). ECM degradation requires metalloproteases, but whether other enzymes are required is unclear. Here, we show that GALA induces th...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/s41556-020-00590-w
更新日期:2020-11-01 00:00:00
abstract::Lysosomes respond to environmental cues by controlling their own biogenesis, but the underlying mechanisms are poorly understood. Here we describe a protein kinase C (PKC)-dependent and mTORC1-independent mechanism for regulating lysosome biogenesis, which provides insights into previously reported effects of PKC on l...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3407
更新日期:2016-10-01 00:00:00
abstract::The migration of border cells during Drosophila melanogaster oogenesis is a simple and powerful system for studying invasive cell migration in vivo. Border cells are somatic cells that delaminate from the follicular epithelium of an egg chamber and invade the germ line cluster. They migrate between the nurse cells to ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb848
更新日期:2002-09-01 00:00:00
abstract::Neural Wiskott-Aldrich syndrome protein (N-WASP) and WAVE are members of a family of proteins that use the Arp2/3 complex to stimulate actin assembly in actin-based motile processes. By entering into distinct macromolecular complexes, they act as convergent nodes of different signalling pathways. The role of WAVE in g...
journal_title:Nature cell biology
pub_type: 信件
doi:10.1038/ncb1304
更新日期:2005-10-01 00:00:00
abstract::The importance of herpes simplex viruses (HSV) as human pathogens and the emerging prospect of using mutant derivatives of HSV-1 as potential anti-cancer therapeutics have necessitated a thorough investigation into the molecular basis of host-cell permissiveness to HSV. Here we show that NIH-3T3 cells transformed with...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35087061
更新日期:2001-08-01 00:00:00
abstract::Protein ubiquitination regulates many cellular processes, including protein degradation, signal transduction, DNA repair and cell division. In the classical model, a uniform polyubiquitin chain that is linked through Lys 48 is required for recognition and degradation by the 26S proteasome. Here, we used a reconstitute...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1436
更新日期:2006-07-01 00:00:00
abstract::The contribution of autophagy to cancer development remains controversial, largely owing to the fact that autophagy can be tumour suppressive or oncogenic in different biological contexts. Here, we show that in non-small-cell lung cancer (NSCLC), casein kinase 1 alpha 1 (CK1α) suppresses tumour growth by functioning a...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/s41556-018-0065-8
更新日期:2018-04-01 00:00:00
abstract::Haematopoietic stem-progenitor cells (HSPCs) reside in the bone marrow niche, where interactions with osteoblasts provide essential cues for their proliferation and survival. Here, we use live-cell imaging to characterize both the site of contact between osteoblasts and haematopoietic progenitor cells (HPCs) and event...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1838
更新日期:2009-03-01 00:00:00
abstract::Cellular transformation and cancer progression is accompanied by changes in the metabolic landscape. Master co-regulators of metabolism orchestrate the modulation of multiple metabolic pathways through transcriptional programs, and hence constitute a probabilistically parsimonious mechanism for general metabolic rewir...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3357
更新日期:2016-06-01 00:00:00
abstract::Blocking mitotic progression has been proposed as an attractive therapeutic strategy to impair proliferation of tumour cells. However, how cells survive during prolonged mitotic arrest is not well understood. We show here that survival during mitotic arrest is affected by the special energetic requirements of mitotic ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3231
更新日期:2015-10-01 00:00:00
abstract::Nucleocytoplasmic transport occurs through nuclear pore complexes (NPCs) embedded in the nuclear envelope. Here, we discovered an unexpected role for yeast dynein light chain (Dyn2) in the NPC. Dyn2 is a previously undescribed nucleoporin that functions as molecular glue to dimerize and stabilize the Nup82-Nsp1-Nup159...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1604
更新日期:2007-07-01 00:00:00
abstract::Ciliates and flagellates temporarily swim backwards on collision by generating a mechanoreceptor potential. Although this potential has been shown to be associated with cilia in Paramecium, the molecular entity of the mechanoreceptor has remained unknown. Here we show that Chlamydomonas cells express TRP11, a member o...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2214
更新日期:2011-05-01 00:00:00
abstract::Microtubules are central elements of the eukaryotic cytoskeleton that often function as part of branched networks. Current models for branching include nucleation of new microtubules from severed microtubule seeds or from γ-tubulin recruited to the side of a pre-existing microtubule. Here, we found that microtubules c...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/s41556-018-0199-8
更新日期:2018-10-01 00:00:00
abstract::Collective cell migration is essential for both physiological and pathological processes. Adherens junctions (AJs) maintain the integrity of the migrating cell group and promote cell coordination while allowing cellular rearrangements. Here, we show that AJs undergo a continuous treadmilling along the lateral sides of...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2985
更新日期:2014-07-01 00:00:00
abstract::Micronucleation of missegregated chromatin can lead to substantial chromosome rearrangements via chromothripsis. However, the molecular details of micronucleus-based chromothripsis are still unclear. Now, an elegant system that specifically induces missegregation of the Y chromosome provides insight into this process,...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3458
更新日期:2016-12-23 00:00:00
abstract::Cancer-associated fibroblasts (CAFs) promote tumour invasion and metastasis. We show that CAFs exert a physical force on cancer cells that enables their collective invasion. Force transmission is mediated by a heterophilic adhesion involving N-cadherin at the CAF membrane and E-cadherin at the cancer cell membrane. Th...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3478
更新日期:2017-03-01 00:00:00
abstract::Actin dynamics is a tightly regulated process involved in various cellular events including biogenesis of clathrin-coated, AP-1 (adaptor protein 1)-coated transport carriers connecting the trans-Golgi network (TGN) and the endocytic pathway. However, the mechanisms coordinating coat assembly, membrane and actin remode...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2034
更新日期:2010-04-01 00:00:00