Direct induction of microtubule branching by microtubule nucleation factor SSNA1.

Abstract:

:Microtubules are central elements of the eukaryotic cytoskeleton that often function as part of branched networks. Current models for branching include nucleation of new microtubules from severed microtubule seeds or from γ-tubulin recruited to the side of a pre-existing microtubule. Here, we found that microtubules can be directly remodelled into branched structures by the microtubule-remodelling factor SSNA1 (also known as NA14 or DIP13). The branching activity of SSNA1 relies on its ability to self-assemble into fibrils in a head-to-tail fashion. SSNA1 fibrils guide protofilaments of a microtubule to split apart to form daughter microtubules. We further found that SSNA1 localizes at axon branching sites and has a key role in neuronal development. SSNA1 mutants that abolish microtubule branching in vitro also fail to promote axon development and branching when overexpressed in neurons. We have, therefore, discovered a mechanism for microtubule branching and implicated its role in neuronal development.

journal_name

Nat Cell Biol

journal_title

Nature cell biology

authors

Basnet N,Nedozralova H,Crevenna AH,Bodakuntla S,Schlichthaerle T,Taschner M,Cardone G,Janke C,Jungmann R,Magiera MM,Biertümpfel C,Mizuno N

doi

10.1038/s41556-018-0199-8

subject

Has Abstract

pub_date

2018-10-01 00:00:00

pages

1172-1180

issue

10

eissn

1465-7392

issn

1476-4679

pii

10.1038/s41556-018-0199-8

journal_volume

20

pub_type

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