PI-loting membrane traffic.

abstract：:MicroRNAs (miRNAs) are increasingly implicated in regulating the malignant progression of cancer. Here we show that miR-9, which is upregulated in breast cancer cells, directly targets CDH1, the E-cadherin-encoding messenger RNA, leading to increased cell motility and invasiveness. miR-9-mediated E-cadherin downregula...

journal_title：Nature cell biology

authors： Ma L,Young J,Prabhala H,Pan E,Mestdagh P,Muth D,Teruya-Feldstein J,Reinhardt F,Onder TT,Valastyan S,Westermann F,Speleman F,Vandesompele J,Weinberg RA

更新日期：2010-03-01 00:00:00

abstract：:The molecular requirements and morphology of migrating cells can vary depending on matrix geometry; therefore, predicting the optimal migration strategy or the effect of experimental perturbation is difficult. We present a model of cell motility that encompasses actin-polymerization-based protrusions, actomyosin contr...

journal_title：Nature cell biology

authors： Tozluoğlu M,Tournier AL,Jenkins RP,Hooper S,Bates PA,Sahai E

更新日期：2013-07-01 00:00:00

abstract：:The TOR (target of rapamycin) kinase limits longevity by poorly understood mechanisms. Rapamycin suppresses the mammalian TORC1 complex, which regulates translation, and extends lifespan in diverse species, including mice. We show that rapamycin selectively blunts the pro-inflammatory phenotype of senescent cells. Cel...

journal_title：Nature cell biology

authors： Laberge RM,Sun Y,Orjalo AV,Patil CK,Freund A,Zhou L,Curran SC,Davalos AR,Wilson-Edell KA,Liu S,Limbad C,Demaria M,Li P,Hubbard GB,Ikeno Y,Javors M,Desprez PY,Benz CC,Kapahi P,Nelson PS,Campisi J

更新日期：2015-08-01 00:00:00

abstract：:Nitric oxide (NO) is a pleiotropic signalling molecule that binds to cytochrome c oxidase (complex IV) reversibly and in competition with oxygen. This action of NO has both physiological and pathophysiological consequences. Here we report that endogenously generated NO, which disrupts the respiratory chain, may cause ...

journal_title：Nature cell biology

authors： Xu W,Liu L,Charles IG,Moncada S

更新日期：2004-11-01 00:00:00

abstract：:During meiosis, homologous chromosomes are sorted into pairs and are then intimately aligned, or synapsed, along their lengths while a proteinaceous structure, the synaptonemal complex, is assembled between them. However, little is known about how chromosomes first recognise each other. Here we show, by comparing the ...

journal_title：Nature cell biology

authors： Prieto P,Shaw P,Moore G

更新日期：2004-09-01 00:00:00

abstract：:MAPKAP kinase 2 (MK2) is one of several kinases that are regulated through direct phosphorylation by p38 MAP kinase. By introducing a targeted mutation into the mouse MK2 gene, we have determined the physiological function of MK2 in vivo. Mice that lack MK2 show increased stress resistance and survive LPS-induced endo...

journal_title：Nature cell biology

authors： Kotlyarov A,Neininger A,Schubert C,Eckert R,Birchmeier C,Volk HD,Gaestel M

更新日期：1999-06-01 00:00:00

abstract：:Pyruvate kinase M2 (PKM2) is upregulated in multiple cancer types and contributes to the Warburg effect by unclear mechanisms. Here we demonstrate that EGFR-activated ERK2 binds directly to PKM2 Ile 429/Leu 431 through the ERK2 docking groove and phosphorylates PKM2 at Ser 37, but does not phosphorylate PKM1. Phosphor...

journal_title：Nature cell biology

authors： Yang W,Zheng Y,Xia Y,Ji H,Chen X,Guo F,Lyssiotis CA,Aldape K,Cantley LC,Lu Z

更新日期：2012-12-01 00:00:00

abstract：:Brown adipose tissue is a key metabolic organ that oxidizes fatty acids and glucose to generate heat. Through epigenomic analyses of multiple adipose depots, the transcription factor nuclear factor I-A (NFIA) is now shown to drive the brown fat genetic program through binding to lineage-specific cis-regulatory element...

journal_title：Nature cell biology

authors： Shapira SN,Seale P

更新日期：2017-08-31 00:00:00

abstract：:Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) involves a marked reorganization of chromatin. To identify post-translational histone modifications that change in global abundance during this process, we have applied a quantitative mass-spectrometry-based approach. We found that iPSCs, compa...

journal_title：Nature cell biology

authors： Sridharan R,Gonzales-Cope M,Chronis C,Bonora G,McKee R,Huang C,Patel S,Lopez D,Mishra N,Pellegrini M,Carey M,Garcia BA,Plath K

更新日期：2013-07-01 00:00:00

abstract：:Canonical Wnt signalling has been implicated in mouse and human embryonic stem cell (ESC) maintenance; however, its requirement is controversial. β-catenin is the key component in this highly conserved Wnt pathway, acting as a transcriptional transactivator. However, β-catenin has additional roles at the plasma membra...

journal_title：Nature cell biology

authors： Lyashenko N,Winter M,Migliorini D,Biechele T,Moon RT,Hartmann C

更新日期：2011-06-19 00:00:00

abstract：:Understanding the factors that direct tissue organization during development is one of the most fundamental goals in developmental biology. Various hypotheses explain cell sorting and tissue organization on the basis of the adhesive and mechanical properties of the constituent cells. However, validating these hypothes...

journal_title：Nature cell biology

authors： Krieg M,Arboleda-Estudillo Y,Puech PH,Käfer J,Graner F,Müller DJ,Heisenberg CP

更新日期：2008-04-01 00:00:00

abstract：:For accurate segregation of chromosomes during cell division, microtubule fibres must attach sister kinetochores to opposite poles of the mitotic spindle (bi-orientation). Aurora kinases are linked to oncogenesis and have been implicated in the regulation of chromosome-microtubule attachments. Although loss of Aurora ...

journal_title：Nature cell biology

authors： Lampson MA,Renduchitala K,Khodjakov A,Kapoor TM

更新日期：2004-03-01 00:00:00

abstract：:During interphase, centrosomes are held together by a proteinaceous linker that connects the proximal ends of the mother and daughter centriole. This linker is disassembled at the onset of mitosis in a process known as centrosome disjunction, thereby facilitating centrosome separation and bipolar spindle formation. Th...

journal_title：Nature cell biology

authors： Mardin BR,Lange C,Baxter JE,Hardy T,Scholz SR,Fry AM,Schiebel E

更新日期：2010-12-01 00:00:00

abstract：:The pericentriolar material (PCM), the microtubule-organizing component of the centrosome, contains a multitude of proteins and is commonly described as an amorphous cloud surrounding the centrioles. However, the days of the PCM as an unstructured matrix are numbered. Using super-resolution microscopy, several reports...

journal_title：Nature cell biology

authors： Lüders J

更新日期：2012-11-01 00:00:00

abstract：:A number of key regulators of mouse embryonic stem (ES) cell identity, including the transcription factor Nanog, show strong expression fluctuations at the single-cell level. The molecular basis for these fluctuations is unknown. Here we used a genetic complementation strategy to investigate expression changes during ...

journal_title：Nature cell biology

authors： MacArthur BD,Sevilla A,Lenz M,Müller FJ,Schuldt BM,Schuppert AA,Ridden SJ,Stumpf PS,Fidalgo M,Ma'ayan A,Wang J,Lemischka IR

更新日期：2012-11-01 00:00:00

abstract：:RNA interference has become an indispensable tool for loss-of-function studies across eukaryotes. By enabling stable and reversible gene silencing, shRNAs provide a means to study long-term phenotypes, perform pool-based forward genetic screens and examine the consequences of temporary target inhibition in vivo. Howev...

journal_title：Nature cell biology

authors： Fellmann C,Lowe SW

更新日期：2014-01-01 00:00:00

abstract：:PX domains are found in a variety of proteins that associate with cell membranes, but their molecular function has remained obscure. We show here that the PX domains in p47phox and p40phox subunits of the phagocyte NADPH oxidase bind to phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4)P(2)) and phosphatidylinositol-3...

journal_title：Nature cell biology

authors： Kanai F,Liu H,Field SJ,Akbary H,Matsuo T,Brown GE,Cantley LC,Yaffe MB

更新日期：2001-07-01 00:00:00

abstract：:Actomyosin contractility is a mechanism by which cells exert locomotory force against their environment. Signalling downstream of the small GTPase Rho increases contractility through Rho-kinase (ROCK)-mediated regulation of myosin-II light chain (MLC2) phosphorylation. Cdc42 signalling has been shown to control cell p...

journal_title：Nature cell biology

authors： Wilkinson S,Paterson HF,Marshall CJ

更新日期：2005-03-01 00:00:00

abstract：:Regulated activation of integrins is critical for cell adhesion, motility and tissue homeostasis. Talin and kindlins activate β1-integrins, but the counteracting inhibiting mechanisms are poorly defined. We identified SHARPIN as an important inactivator of β1-integrins in an RNAi screen. SHARPIN inhibited β1-integrin ...

journal_title：Nature cell biology

authors： Rantala JK,Pouwels J,Pellinen T,Veltel S,Laasola P,Mattila E,Potter CS,Duffy T,Sundberg JP,Kallioniemi O,Askari JA,Humphries MJ,Parsons M,Salmi M,Ivaska J

更新日期：2011-09-25 00:00:00

abstract：:Senescence, a persistent form of cell-cycle arrest, is often associated with a diverse secretome, which provides complex functionality for senescent cells within the tissue microenvironment. We show that oncogene-induced senescence is accompanied by a dynamic fluctuation of NOTCH1 activity, which drives a TGF-β-rich s...

journal_title：Nature cell biology

authors： Hoare M,Ito Y,Kang TW,Weekes MP,Matheson NJ,Patten DA,Shetty S,Parry AJ,Menon S,Salama R,Antrobus R,Tomimatsu K,Howat W,Lehner PJ,Zender L,Narita M

更新日期：2016-09-01 00:00:00

abstract：:Epigenetic mechanisms participate in the regulation of gene transcription in eukaryotes. Two studies in yeast have revealed an additional mechanism for controlling global gene transcription that is based on an inherited self-perpetuating change in the conformation of two different components of key transcriptional reg...

journal_title：Nature cell biology

authors： Tuite MF,Cox BS

更新日期：2009-03-01 00:00:00

abstract：:We have designed new non-peptidic potential inhibitors of gamma-secretase and examined their ability to prevent production of amyloid-beta 40 (Abeta40) and Abeta42 by human cells expressing wild-type and Swedish-mutant beta-amyloid precursor protein (betaAPP). Here we identify three such agents that markedly reduce re...

journal_title：Nature cell biology

authors： Petit A,Bihel F,Alvès da Costa C,Pourquié O,Checler F,Kraus JL

更新日期：2001-05-01 00:00:00

abstract：:The mitochondrial inner membrane is the central energy-converting membrane of eukaryotic cells. The electrochemical proton gradient generated by the respiratory chain drives the ATP synthase. To maintain this proton-motive force, the inner membrane forms a tight barrier and strictly controls the translocation of ions....

journal_title：Nature cell biology

authors： van der Laan M,Meinecke M,Dudek J,Hutu DP,Lind M,Perschil I,Guiard B,Wagner R,Pfanner N,Rehling P

更新日期：2007-10-01 00:00:00

abstract：:Wiskott-Aldrich syndrome protein (WASP) and N-WASP have emerged as key proteins connecting signalling cascades to actin polymerization. Here we show that the amino-terminal WH1 domain, and not the polyproline-rich region, of N-WASP is responsible for its recruitment to sites of actin polymerization during Cdc42-indepe...

journal_title：Nature cell biology

authors： Moreau V,Frischknecht F,Reckmann I,Vincentelli R,Rabut G,Stewart D,Way M

更新日期：2000-07-01 00:00:00

abstract：:Focal adhesions are integrin-based multiprotein complexes, several micrometres in diameter, that mechanically link the extracellular matrix with the termini of actin bundles. The molecular diversity of focal adhesions and their role in cell migration and matrix sensing has been extensively studied, but their ultrastru...

journal_title：Nature cell biology

authors： Patla I,Volberg T,Elad N,Hirschfeld-Warneken V,Grashoff C,Fässler R,Spatz JP,Geiger B,Medalia O

更新日期：2010-09-01 00:00:00

abstract：:Small RNAs generated at DNA break sites are implicated in mammalian DNA repair. Now, a study shows that following the formation of DNA double-strand breaks, bidirectional transcription events adjacent to the break generate small RNAs that trigger the DNA damage response by local RNA:RNA interactions. ...

journal_title：Nature cell biology

authors： Storici F,Tichon AE

更新日期：2017-11-29 00:00:00

abstract：:Aurora A, an integral mitotic kinase, is essential for microtubule dynamics of post-mitotic neurons. PKCzeta activates Aurora A, which in turn phosphorylates NDEL1 to promote neurite extension. This raises the possibility that Aurora A may also be involved in establishing cell polarity and axon/dendrite elaboration in...

journal_title：Nature cell biology

authors： Lefkowitz GK,Gleeson JG

更新日期：2009-09-01 00:00:00

abstract：:The presence of actin in the nucleus has been well established, and several studies have implicated nuclear actin in transcriptional regulation. Neuronal Wiskott-Aldrich syndrome protein (N-WASP) is a member of the WASP family of proteins; these proteins function in the cytoplasm as key regulators of cortical actin fi...

journal_title：Nature cell biology

authors： Wu X,Yoo Y,Okuhama NN,Tucker PW,Liu G,Guan JL

更新日期：2006-07-01 00:00:00

abstract：:The polarity protein Scribble (SCRIB) regulates apical-basal polarity, directional migration and tumour suppression in Drosophila and mammals. Here we report that SCRIB is an important regulator of myeloid cell functions including bacterial infection and inflammation. SCRIB interacts directly with the NADPH oxidase (N...

journal_title：Nature cell biology

authors： Zheng W,Umitsu M,Jagan I,Tran CW,Ishiyama N,BeGora M,Araki K,Ohashi PS,Ikura M,Muthuswamy SK

更新日期：2016-11-01 00:00:00

abstract：:Store-operated Ca(2+) channels (SOCs) are activated in response to Ca(2+) release from the endoplasmic reticulum (ER). The stromal interaction molecule 1 (STIM1) is the ER sensor that transmits the stored Ca(2+) content to the pore-forming SOCs Orai and TRPC channels. Recent studies reveal high levels of Orai1 and STI...

journal_title：Nature cell biology

authors： Shin DM,Muallem S

更新日期：2008-06-01 00:00:00