Abstract：
:The presence of actin in the nucleus has been well established, and several studies have implicated nuclear actin in transcriptional regulation. Neuronal Wiskott-Aldrich syndrome protein (N-WASP) is a member of the WASP family of proteins; these proteins function in the cytoplasm as key regulators of cortical actin filament. Interestingly, N-WASP has also been observed in the nucleus. However, a potential nuclear function for N-WASP has not been established. Here, we report the identification of nuclear N-WASP within a large nuclear-protein complex containing PSF-NonO (polypyrimidine-tract-binding-protein-associated splicing factor-non-Pou-domain octamer-binding protein/p54(nrb)), nuclear actin and RNA polymerase II. The PSF-NonO complex is involved in the regulation of many cellular processes, such as transcription, RNA processing, DNA unwinding and repair. We demonstrate that the interaction of N-WASP with the PSF-NonO complex can couple N-WASP with RNA polymerase II to regulate transcription. We also provide evidence that the potential function of N-WASP in promoting polymerization of nuclear actins has an important role in this process. Based on these results, we propose a nuclear function for N-WASP in transcriptional regulation.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Wu X,Yoo Y,Okuhama NN,Tucker PW,Liu G,Guan JLdoi
10.1038/ncb1433subject
Has Abstractpub_date
2006-07-01 00:00:00pages
756-63issue
7eissn
1465-7392issn
1476-4679pii
ncb1433journal_volume
8pub_type
杂志文章abstract：:Symmetrical cell division requires duplication of DNA and protein content to generate two daughter cells. Centrosomes also duplicate during cell division, but the mechanism controlling this process is incompletely understood. We describe an alternative splice form of SadB encoding a short SADB Ser/Thr kinase whose act...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1921
更新日期：2009-09-01 00:00:00
abstract：:Despite advances in the differentiation of insulin-producing cells from human embryonic stem cells, the generation of mature functional β cells in vitro has remained elusive. To accomplish this goal, we have developed cell culture conditions to closely mimic events occurring during pancreatic islet organogenesis and β...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-018-0271-4
更新日期：2019-02-01 00:00:00
abstract：:Current models for the export of messenger RNA share the notion that the highly abundant class of nuclear RNA-binding proteins--the hnRNP proteins--have a key role in exporting RNA. But recent studies have led to a new understanding of several non-hnRNP proteins, including SR proteins and the conserved mRNA export fac...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb0901-e201
更新日期：2001-09-01 00:00:00
abstract：:Activation of mitosis-promoting factor (MPF) drives mitotic commitment. In human cells active MPF appears first on centrosomes. We show that local activation of MPF on the equivalent organelle of fission yeast, the spindle pole body (SPB), promotes Polo kinase activity at the SPBs long before global MPF activation dri...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2633
更新日期：2013-01-01 00:00:00
abstract：:Tissue stem cells are the cell of origin for many malignancies. Metabolites regulate the balance between self-renewal and differentiation, but whether endogenous metabolic pathways or nutrient availability predispose stem cells towards transformation remains unknown. Here, we address this question in epidermal stem ce...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-020-0525-9
更新日期：2020-07-01 00:00:00
abstract：:The emergence of resistance to poly-ADP-ribose polymerase inhibitors (PARPi) poses a threat to the treatment of BRCA1 and BRCA2 (BRCA1/2)-deficient tumours. Stabilization of stalled DNA replication forks is a recently identified PARPi-resistance mechanism that promotes genomic stability in BRCA1/2-deficient cancers. D...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3626
更新日期：2017-11-01 00:00:00
abstract：:The Drosophila fruitless (fru) gene product Fru has been postulated to be a neural sex-determination factor that directs the development of at least two male-specific characteristics, namely courtship behaviour and formation of the muscle of Lawrence (MOL). The fru gene encodes a putative transcription factor with a B...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35019537
更新日期：2000-08-01 00:00:00
abstract：:The rising incidence of obesity and related disorders such as diabetes and heart disease has focused considerable attention on the discovery of new therapeutics. One promising approach has been to increase the number or activity of brown-like adipocytes in white adipose depots, as this has been shown to prevent diet-i...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3075
更新日期：2015-01-01 00:00:00
abstract：:Guard cells, which form stomata in leaf epidermes, sense a multitude of environmental signals and integrate this information to regulate stomatal movements. Compared with the advanced understanding of light and water stress responses in guard cells, the molecular mechanisms that underlie stomatal CO(2) signalling have...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1387
更新日期：2006-04-01 00:00:00
abstract：:The deposition of chemical modifications into RNA is a crucial regulator of temporal and spatial gene expression programs during development. Accordingly, altered RNA modification patterns are widely linked to developmental diseases. Recently, the dysregulation of RNA modification pathways also emerged as a contributo...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/s41556-019-0319-0
更新日期：2019-05-01 00:00:00
abstract：:Cohesin confers both intrachromatid and interchromatid cohesion through formation of a tripartite ring within which DNA is thought to be entrapped. Here, I discuss what is known about the four stages of the cohesin ring cycle using the ring model as an intellectual framework. I postulate that cohesin loading onto chro...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb2349
更新日期：2011-10-03 00:00:00
abstract：:The physical characteristics of tumours are intricately linked to the tumour phenotype and difficulties during treatment. Many factors contribute to the increased stiffness of tumours; from increased matrix deposition, matrix remodelling by forces from cancer cells and stromal fibroblasts, matrix crosslinking, increas...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/s41556-018-0131-2
更新日期：2018-07-01 00:00:00
abstract：:Topoisomerase II (Topo II) is required to separate intertwined sister chromatids before chromosome segregation can occur in mitosis. However, it remains to be resolved whether Topo II has any role in checkpoint control. Here we report that when phosphorylated, Ser 1524 of Topo IIalpha acts as a binding site for the BR...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1828
更新日期：2009-02-01 00:00:00
abstract：:Direct reprogramming by forced expression of transcription factors can convert one cell type into another. Thus, desired cell types can be generated bypassing pluripotency. However, direct reprogramming towards renal cells remains an unmet challenge. Here, we identify renal cell fate-inducing factors on the basis of t...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3437
更新日期：2016-12-01 00:00:00
abstract：:The molecular requirements and morphology of migrating cells can vary depending on matrix geometry; therefore, predicting the optimal migration strategy or the effect of experimental perturbation is difficult. We present a model of cell motility that encompasses actin-polymerization-based protrusions, actomyosin contr...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2775
更新日期：2013-07-01 00:00:00
abstract：:Metazoan internal organs are assembled from polarized tubular epithelia that must set aside an apical membrane domain as a lumenal surface. In a global Caenorhabditis elegans tubulogenesis screen, interference with several distinct fatty-acid-biosynthetic enzymes transformed a contiguous central intestinal lumen into ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2328
更新日期：2011-09-18 00:00:00
abstract：:The importance of herpes simplex viruses (HSV) as human pathogens and the emerging prospect of using mutant derivatives of HSV-1 as potential anti-cancer therapeutics have necessitated a thorough investigation into the molecular basis of host-cell permissiveness to HSV. Here we show that NIH-3T3 cells transformed with...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35087061
更新日期：2001-08-01 00:00:00
abstract：:The SNF2h (sucrose non-fermenting protein 2 homologue)-containing chromatin-remodelling complex NoRC silences a fraction of ribosomal RNA genes (rDNA) by establishing a heterochromatic structure at the rDNA promoter. Here we show that the acetyltransferase MOF (males absent on the first) acetylates TIP5, the largest s...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1914
更新日期：2009-08-01 00:00:00
abstract：:Reference datasets should be accessible independently of scientific papers in a citable form, allowing attribution. ...
journal_title：Nature cell biology
pub_type： 社论
doi：10.1038/ncb1109-1273a
更新日期：2009-11-01 00:00:00
abstract：:Loss of spindle-pole integrity during mitosis leads to multipolarity independent of centrosome amplification. Multipolar-spindle conformation favours incorrect kinetochore-microtubule attachments, compromising faithful chromosome segregation and daughter-cell viability. Spindle-pole organization influences and is infl...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2423
更新日期：2012-02-05 00:00:00
abstract：:The intracellular Ca(2+) concentration ([Ca(2+)](i)) in skeletal muscles must be rapidly regulated during the excitation-contraction-relaxation process. However, the signalling components involved in such rapid Ca(2+) movement are not fully understood. Here we report that mice deficient in the newly identified PtdInsP...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1884
更新日期：2009-06-01 00:00:00
abstract：:Metaphase spindles are microtubule-based structures that use a multitude of proteins to modulate their morphology and function. Today, we understand many details of microtubule assembly, the role of microtubule-associated proteins, and the action of molecular motors. Ultimately, the challenge remains to understand how...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2834
更新日期：2013-09-01 00:00:00
abstract：:The stability and membrane localization of the transforming growth factor-β (TGF-β) type I receptor (TβRI) determines the levels of TGF-β signalling. TβRI is targeted for ubiquitylation-mediated degradation by the SMAD7-SMURF2 complex. Here we performed a genome-wide gain-of-function screen and identified ubiquitin-sp...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2522
更新日期：2012-06-17 00:00:00
abstract：:The substrates used to modify nucleic acids and chromatin are affected by nutrient availability and the activity of metabolic pathways. Thus, cellular metabolism constitutes a fundamental component of chromatin status and thereby of genome regulation. Here we describe the biochemical and genetic principles of how meta...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb3629
更新日期：2017-11-01 00:00:00
abstract：:The endoplasmic reticulum (ER), a network of membranous sheets and pipes, supports functions encompassing biogenesis of secretory proteins and delivery of functional solutes throughout the cell1,2. Molecular mobility through the ER network enables these functionalities, but diffusion alone is not sufficient to explain...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-018-0192-2
更新日期：2018-10-01 00:00:00
abstract：:Robust mechanisms to control cell proliferation have evolved to maintain the integrity of organ architecture. Here, we investigated how two critical proliferative pathways, Myc and E2f, are integrated to control cell cycles in normal and Rb-deficient cells using a murine intestinal model. We show that Myc and E2f1-3 h...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3210
更新日期：2015-08-01 00:00:00
abstract：:Fetal haematopoietic stem cells (HSCs) self-renew extensively to build the blood system from scratch. The protein Lin28b negatively regulates the microRNA let-7 to keep levels of its target Hmga2 high, hence conferring high self-renewal potential to fetal HSCs. This regulatory circuit can be experimentally modulated t...
journal_title：Nature cell biology
pub_type： 评论,新闻
doi：10.1038/ncb2817
更新日期：2013-08-01 00:00:00
abstract：:Phosphoinositides (PIs) undergo phosphorylation/dephosphorylation cycles through organelle-specific PI kinases and PI phosphatases that lead to distinct subcellular distributions of the individual PI species. Specific PIs control the correct timing and location of many trafficking events. Their ultimate mode of action...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb0604-487
更新日期：2004-06-01 00:00:00
abstract：:Here we investigate the role of the control of apoptosis in normal cell division. We show that interference with the expression or function of the apoptosis inhibitor survivin causes caspase-dependent cell death in the G2/M phase of the cell cycle, and a cell-division defect characterized by centrosome dysregulation, ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/70242
更新日期：1999-12-01 00:00:00
abstract：:Centromeres are specialized chromosomal domains that direct kinetochore assembly during mitosis. CENP-A (centromere protein A), a histone H3-variant present exclusively in centromeric nucleosomes, is thought to function as an epigenetic mark that specifies centromere identity. Here we identify the essential centromere...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1899
更新日期：2009-07-01 00:00:00
