Abstract:
:The endoplasmic reticulum (ER), a network of membranous sheets and pipes, supports functions encompassing biogenesis of secretory proteins and delivery of functional solutes throughout the cell1,2. Molecular mobility through the ER network enables these functionalities, but diffusion alone is not sufficient to explain luminal transport across supramicrometre distances. Understanding the ER structure-function relationship is critical in light of mutations in ER morphology-regulating proteins that give rise to neurodegenerative disorders3,4. Here, super-resolution microscopy and analysis of single particle trajectories of ER luminal proteins revealed that the topological organization of the ER correlates with distinct trafficking modes of its luminal content: with a dominant diffusive component in tubular junctions and a fast flow component in tubules. Particle trajectory orientations resolved over time revealed an alternating current of the ER contents, while fast ER super-resolution identified energy-dependent tubule contraction events at specific points as a plausible mechanism for generating active ER luminal flow. The discovery of active flow in the ER has implications for timely ER content distribution throughout the cell, particularly important for cells with extensive ER-containing projections such as neurons.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Holcman D,Parutto P,Chambers JE,Fantham M,Young LJ,Marciniak SJ,Kaminski CF,Ron D,Avezov Edoi
10.1038/s41556-018-0192-2subject
Has Abstractpub_date
2018-10-01 00:00:00pages
1118-1125issue
10eissn
1465-7392issn
1476-4679pii
10.1038/s41556-018-0192-2journal_volume
20pub_type
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