Abstract：
:Symmetrical cell division requires duplication of DNA and protein content to generate two daughter cells. Centrosomes also duplicate during cell division, but the mechanism controlling this process is incompletely understood. We describe an alternative splice form of SadB encoding a short SADB Ser/Thr kinase whose activity fluctuates during the cell cycle, localizes to centrosomes, and controls centrosome duplication. Reduction of endogenous SADB levels diminished centrosome numbers, whereas enhanced SADB expression induced centrosome amplification. SADB exerted this action through phosphorylation of gamma-tubulin on Ser 131, as expression of a phosphomimetic Ser 131-to-Asp gamma-tubulin mutant alone increased centrosome numbers, whereas non-phosphorylatable Ala 131-gamma-tubulin impaired centrosome duplication. We propose that SADB kinase activity controls centrosome homeostasis by regulating phosphorylation of gamma-tubulin.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Alvarado-Kristensson M,Rodríguez MJ,Silió V,Valpuesta JM,Carrera ACdoi
10.1038/ncb1921subject
Has Abstractpub_date
2009-09-01 00:00:00pages
1081-92issue
9eissn
1465-7392issn
1476-4679pii
ncb1921journal_volume
11pub_type
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