当前位置： SCI文献检索 > NATURE CELL BIOLOGY期刊下所有文献 > Molecular basis for the functional interaction of dynein light chain with the nuclear-pore complex.

Molecular basis for the functional interaction of dynein light chain with the nuclear-pore complex.

Abstract：

:Nucleocytoplasmic transport occurs through nuclear pore complexes (NPCs) embedded in the nuclear envelope. Here, we discovered an unexpected role for yeast dynein light chain (Dyn2) in the NPC. Dyn2 is a previously undescribed nucleoporin that functions as molecular glue to dimerize and stabilize the Nup82-Nsp1-Nup159 complex, a module of the cytoplasmic pore filaments. Biochemical analyses showed that Dyn2 binds to a linear motif (termed DID(Nup159)) inserted between the Phe-Gly repeat and coiled-coil domain of Nup159. Electron microscopy revealed that the reconstituted Dyn2-DID(Nup159) complex forms a rigid rod-like structure, in which five Dyn2 homodimers align like 'pearls on a string' between two extented DID(Nup159) strands. These findings imply that the rigid 20 nm long Dyn2-DID(Nup159) filament projects the Nup159 Phe-Gly repeats from the Nup82 module. Thus, it is possible that dynein light chain plays a role in organizing natively unfolded Phe-Gly repeats within the NPC scaffold to facilitate nucleocytoplasmic transport.

journal_name

Nat Cell Biol

journal_title

Nature cell biology

authors

Stelter P,Kunze R,Flemming D,Höpfner D,Diepholz M,Philippsen P,Böttcher B,Hurt E

doi

10.1038/ncb1604

subject

Has Abstract

pub_date

2007-07-01 00:00:00

pages

788-96

issue

7

eissn

1465-7392

issn

1476-4679

pii

ncb1604

journal_volume

9

pub_type

杂志文章

相关文献

NATURE CELL BIOLOGY文献大全
  • Ketogenesis-generated β-hydroxybutyrate is an epigenetic regulator of CD8+ T-cell memory development.

    abstract：:Glycogen has long been considered to have a function in energy metabolism. However, our recent study indicated that glycogen metabolism, directed by cytosolic phosphoenolpyruvate carboxykinase Pck1, controls the formation and maintenance of CD8+ memory T (Tmem) cells by regulating redox homeostasis1. This unusual meta...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/s41556-019-0440-0

    authors： Zhang H,Tang K,Ma J,Zhou L,Liu J,Zeng L,Zhu L,Xu P,Chen J,Wei K,Liang X,Lv J,Xie J,Liu Y,Wan Y,Huang B

    更新日期：2020-01-01 00:00:00

  • A nutrient-induced affinity switch controls mTORC1 activation by its Rag GTPase-Ragulator lysosomal scaffold.

    abstract：:A key step in nutrient sensing is activation of the master growth regulator, mTORC1 kinase, on the lysosomal membrane. Nutrients enable mTORC1 scaffolding by a complex composed of the Rag GTPases (Rags) and Ragulator, but the underlying mechanism of mTORC1 capture is poorly understood. Combining dynamic imaging in cel...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/s41556-018-0148-6

    authors： Lawrence RE,Cho KF,Rappold R,Thrun A,Tofaute M,Kim DJ,Moldavski O,Hurley JH,Zoncu R

    更新日期：2018-09-01 00:00:00

  • Tissue-specific CTCF-cohesin-mediated chromatin architecture delimits enhancer interactions and function in vivo.

    abstract：:The genome is organized via CTCF-cohesin-binding sites, which partition chromosomes into 1-5 megabase (Mb) topologically associated domains (TADs), and further into smaller sub-domains (sub-TADs). Here we examined in vivo an ∼80 kb sub-TAD, containing the mouse α-globin gene cluster, lying within a ∼1 Mb TAD. We find ...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb3573

    authors： Hanssen LLP,Kassouf MT,Oudelaar AM,Biggs D,Preece C,Downes DJ,Gosden M,Sharpe JA,Sloane-Stanley JA,Hughes JR,Davies B,Higgs DR

    更新日期：2017-08-01 00:00:00

  • The PX domains of p47phox and p40phox bind to lipid products of PI(3)K.

    abstract：:PX domains are found in a variety of proteins that associate with cell membranes, but their molecular function has remained obscure. We show here that the PX domains in p47phox and p40phox subunits of the phagocyte NADPH oxidase bind to phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4)P(2)) and phosphatidylinositol-3...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/35083070

    authors： Kanai F,Liu H,Field SJ,Akbary H,Matsuo T,Brown GE,Cantley LC,Yaffe MB

    更新日期：2001-07-01 00:00:00

  • Apicobasal domain identities of expanding tubular membranes depend on glycosphingolipid biosynthesis.

    abstract：:Metazoan internal organs are assembled from polarized tubular epithelia that must set aside an apical membrane domain as a lumenal surface. In a global Caenorhabditis elegans tubulogenesis screen, interference with several distinct fatty-acid-biosynthetic enzymes transformed a contiguous central intestinal lumen into ...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb2328

    authors： Zhang H,Abraham N,Khan LA,Hall DH,Fleming JT,Göbel V

    更新日期：2011-09-18 00:00:00

  • ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect.

    abstract：:Pyruvate kinase M2 (PKM2) is upregulated in multiple cancer types and contributes to the Warburg effect by unclear mechanisms. Here we demonstrate that EGFR-activated ERK2 binds directly to PKM2 Ile 429/Leu 431 through the ERK2 docking groove and phosphorylates PKM2 at Ser 37, but does not phosphorylate PKM1. Phosphor...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb2629

    authors： Yang W,Zheng Y,Xia Y,Ji H,Chen X,Guo F,Lyssiotis CA,Aldape K,Cantley LC,Lu Z

    更新日期：2012-12-01 00:00:00

  • Redeployment of Myc and E2f1-3 drives Rb-deficient cell cycles.

    abstract：:Robust mechanisms to control cell proliferation have evolved to maintain the integrity of organ architecture. Here, we investigated how two critical proliferative pathways, Myc and E2f, are integrated to control cell cycles in normal and Rb-deficient cells using a murine intestinal model. We show that Myc and E2f1-3 h...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb3210

    authors： Liu H,Tang X,Srivastava A,Pécot T,Daniel P,Hemmelgarn B,Reyes S,Fackler N,Bajwa A,Kladney R,Koivisto C,Chen Z,Wang Q,Huang K,Machiraju R,Sáenz-Robles MT,Cantalupo P,Pipas JM,Leone G

    更新日期：2015-08-01 00:00:00

  • The extracellular matrix guides the orientation of the cell division axis.

    abstract：:The cell division axis determines the future positions of daughter cells and is therefore critical for cell fate. The positioning of the division axis has been mostly studied in systems such as embryos or yeasts, in which cell shape is well defined. In these cases, cell shape anisotropy and cell polarity affect spindl...

    journal_title：Nature cell biology

    pub_type： 信件

    doi：10.1038/ncb1307

    authors： Théry M,Racine V,Pépin A,Piel M,Chen Y,Sibarita JB,Bornens M

    更新日期：2005-10-01 00:00:00

  • Skp2: caught in the Akt.

    abstract：:To control cell proliferation, signal transduction needs to regulate the cell-cycle machinery. Recent findings show that Akt - a major kinase that coordinates diverse signalling pathways - phosphorylates Skp2, a subunit of the SCF-Skp2 ubiquitin ligase that targets key cell-cycle regulators. Akt1-dependent phosphoryla...

    journal_title：Nature cell biology

    pub_type： 评论,新闻

    doi：10.1038/ncb0409-377

    authors： Ecker K,Hengst L

    更新日期：2009-04-01 00:00:00

  • Telomere-independent Rap1 is an IKK adaptor and regulates NF-kappaB-dependent gene expression.

    abstract：:We describe a genome-wide gain-of-function screen for regulators of NF-kappaB, and identify Rap1 (Trf2IP), as an essential modulator of NF-kappaB-mediated pathways. NF-kappaB is induced by ectopic expression of Rap1, whereas its activity is inhibited by Rap1 depletion. In addition to localizing on telomeres, mammalian...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb2080

    authors： Teo H,Ghosh S,Luesch H,Ghosh A,Wong ET,Malik N,Orth A,de Jesus P,Perry AS,Oliver JD,Tran NL,Speiser LJ,Wong M,Saez E,Schultz P,Chanda SK,Verma IM,Tergaonkar V

    更新日期：2010-08-01 00:00:00

  • Mutations in the APC tumour suppressor gene cause chromosomal instability.

    abstract：:Two forms of genetic instability have been described in colorectal cancer: microsatellite instability and chromosomal instability. Microsatellite instability results from mutations in mismatch repair genes; chromosomal instability is the hallmark of many colorectal cancers, although it is not completely understood at ...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/35070129

    authors： Fodde R,Kuipers J,Rosenberg C,Smits R,Kielman M,Gaspar C,van Es JH,Breukel C,Wiegant J,Giles RH,Clevers H

    更新日期：2001-04-01 00:00:00

  • Invasive cell migration is initiated by guided growth of long cellular extensions.

    abstract：:The migration of border cells during Drosophila melanogaster oogenesis is a simple and powerful system for studying invasive cell migration in vivo. Border cells are somatic cells that delaminate from the follicular epithelium of an egg chamber and invade the germ line cluster. They migrate between the nurse cells to ...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb848

    authors： Fulga TA,Rørth P

    更新日期：2002-09-01 00:00:00

  • CK1α suppresses lung tumour growth by stabilizing PTEN and inducing autophagy.

    abstract：:The contribution of autophagy to cancer development remains controversial, largely owing to the fact that autophagy can be tumour suppressive or oncogenic in different biological contexts. Here, we show that in non-small-cell lung cancer (NSCLC), casein kinase 1 alpha 1 (CK1α) suppresses tumour growth by functioning a...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/s41556-018-0065-8

    authors： Cai J,Li R,Xu X,Zhang L,Lian R,Fang L,Huang Y,Feng X,Liu X,Li X,Zhu X,Zhang H,Wu J,Zeng M,Song E,He Y,Yin Y,Li J,Li M

    更新日期：2018-04-01 00:00:00

  • Bif-1 interacts with Beclin 1 through UVRAG and regulates autophagy and tumorigenesis.

    abstract：:Autophagy is an evolutionarily conserved 'self-eating' process. Although the genes essential for autophagy (named Atg) have been identified in yeast, the molecular mechanism of how Atg proteins control autophagosome formation in mammalian cells remains to be elucidated. Here, we demonstrate that Bif-1 (also known as E...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1634

    authors： Takahashi Y,Coppola D,Matsushita N,Cualing HD,Sun M,Sato Y,Liang C,Jung JU,Cheng JQ,Mulé JJ,Pledger WJ,Wang HG

    更新日期：2007-10-01 00:00:00

  • Myosin-XVa is required for tip localization of whirlin and differential elongation of hair-cell stereocilia.

    abstract：:Stereocilia are microvilli-derived mechanosensory organelles that are arranged in rows of graded heights on the apical surface of inner-ear hair cells. The 'staircase'-like architecture of stereocilia bundles is necessary to detect sound and head movement, and is achieved through differential elongation of the actin c...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1219

    authors： Belyantseva IA,Boger ET,Naz S,Frolenkov GI,Sellers JR,Ahmed ZM,Griffith AJ,Friedman TB

    更新日期：2005-02-01 00:00:00

  • Lineage specificity of primary cilia in the mouse embryo.

    abstract：:Primary cilia are required for vertebrate cells to respond to specific intercellular signals. Here we define when and where primary cilia appear in the mouse embryo using a transgenic line that expresses ARL13B-mCherry in cilia and Centrin 2-GFP in centrosomes. Primary cilia first appear on cells of the epiblast at E6...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb3091

    authors： Bangs FK,Schrode N,Hadjantonakis AK,Anderson KV

    更新日期：2015-02-01 00:00:00

  • Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling.

    abstract：:Loss-of-function (LOF) mutations in the endothelial cell (EC)-enriched gene endoglin (ENG) cause the human disease hereditary haemorrhagic telangiectasia-1, characterized by vascular malformations promoted by vascular endothelial growth factor A (VEGFA). How ENG deficiency alters EC behaviour to trigger these anomalie...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb3534

    authors： Jin Y,Muhl L,Burmakin M,Wang Y,Duchez AC,Betsholtz C,Arthur HM,Jakobsson L

    更新日期：2017-06-01 00:00:00

  • CRISPR mutagenesis screening of mice.

    abstract：: ...

    journal_title：Nature cell biology

    pub_type： 评论,杂志文章

    doi：10.1038/s41556-018-0224-y

    authors： Serebrenik YV,Shalem O

    更新日期：2018-11-01 00:00:00

  • Breast-cancer-secreted miR-122 reprograms glucose metabolism in premetastatic niche to promote metastasis.

    abstract：:Reprogrammed glucose metabolism as a result of increased glycolysis and glucose uptake is a hallmark of cancer. Here we show that cancer cells can suppress glucose uptake by non-tumour cells in the premetastatic niche, by secreting vesicles that carry high levels of the miR-122 microRNA. High miR-122 levels in the cir...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb3094

    authors： Fong MY,Zhou W,Liu L,Alontaga AY,Chandra M,Ashby J,Chow A,O'Connor ST,Li S,Chin AR,Somlo G,Palomares M,Li Z,Tremblay JR,Tsuyada A,Sun G,Reid MA,Wu X,Swiderski P,Ren X,Shi Y,Kong M,Zhong W,Chen Y,Wang SE

    更新日期：2015-02-01 00:00:00

  • Endoplasmic reticulum chaperone gp96 is required for innate immunity but not cell viability.

    abstract：:Chaperone proteins are thought to promote the correct folding and assembly of newly synthesized proteins and to facilitate restoration of the folded state under environmental conditions that favour protein denaturation. They are among the most ubiquitous and highly conserved of all proteins. The eukaryotic endoplasmic...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1001-891

    authors： Randow F,Seed B

    更新日期：2001-10-01 00:00:00

  • The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres.

    abstract：:In vertebrates, centromeres lack defined sequences and are thought to be propagated by epigenetic mechanisms involving the incorporation of specialized nucleosomes containing the histone H3 variant centromere protein (CENP)-A. However, the precise mechanisms that target CENP-A to centromeres remain poorly understood. ...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1396

    authors： Okada M,Cheeseman IM,Hori T,Okawa K,McLeod IX,Yates JR 3rd,Desai A,Fukagawa T

    更新日期：2006-05-01 00:00:00

  • HIF activation by pH-dependent nucleolar sequestration of VHL.

    abstract：:Hypoxia and acidosis occur in a wide variety of physiological and pathological settings that include muscle stress, tumour development and ischaemic disorders. A central element in the adaptive response to cellular hypoxia is HIF (hypoxia-inducible factor), a transcription factor that activates an array of genes impli...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1144

    authors： Mekhail K,Gunaratnam L,Bonicalzi ME,Lee S

    更新日期：2004-07-01 00:00:00

  • Sin1 phosphorylation impairs mTORC2 complex integrity and inhibits downstream Akt signalling to suppress tumorigenesis.

    abstract：:The mechanistic target of rapamycin (mTOR) functions as a critical regulator of cellular growth and metabolism by forming multi-component, yet functionally distinct complexes mTORC1 and mTORC2. Although mTORC2 has been implicated in mTORC1 activation, little is known about how mTORC2 is regulated. Here we report that ...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb2860

    authors： Liu P,Gan W,Inuzuka H,Lazorchak AS,Gao D,Arojo O,Liu D,Wan L,Zhai B,Yu Y,Yuan M,Kim BM,Shaik S,Menon S,Gygi SP,Lee TH,Asara JM,Manning BD,Blenis J,Su B,Wei W

    更新日期：2013-11-01 00:00:00

  • VHL takes HIF's breath away.

    abstract：:Inactivation of the von Hippel-Lindau (VHL) tumour-suppressor protein (pVHL) is associated with the von Hippel-Lindau cancer syndrome and the majority of kidney cancers. New evidence suggests that pVHL has properties of an F-box protein that targets the alpha-subunits of hypoxia-inducible factor (HIF)-1 for oxygen-dep...

    journal_title：Nature cell biology

    pub_type： 评论,新闻

    doi：10.1038/35017129

    authors： Krek W

    更新日期：2000-07-01 00:00:00

  • Discrete interactions in cell adhesion measured by single-molecule force spectroscopy.

    abstract：:Cell-cell adhesion mediated by specific cell-surface molecules is essential for multicellular development. Here we quantify de-adhesion forces at the resolution of individual cell-adhesion molecules, by controlling the interactions between single cells and combining single-molecule force spectroscopy with genetic mani...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/35014000

    authors： Benoit M,Gabriel D,Gerisch G,Gaub HE

    更新日期：2000-06-01 00:00:00

  • LRRC31 inhibits DNA repair and sensitizes breast cancer brain metastasis to radiation therapy.

    abstract：:Breast cancer brain metastasis (BCBM) is a devastating disease. Radiation therapy remains the mainstay for treatment of this disease. Unfortunately, its efficacy is limited by the dose that can be safely applied. One promising approach to overcoming this limitation is to sensitize BCBMs to radiation by inhibiting thei...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/s41556-020-00586-6

    authors： Chen Y,Jiang T,Zhang H,Gou X,Han C,Wang J,Chen AT,Ma J,Liu J,Chen Z,Jing X,Lei H,Wang Z,Bao Y,Baqri M,Zhu Y,Bindra RS,Hansen JE,Dou J,Huang C,Zhou J

    更新日期：2020-10-01 00:00:00

  • Rotation and asymmetry of the mitotic spindle direct asymmetric cell division in the developing central nervous system.

    abstract：:The asymmetric segregation of cell-fate determinants and the generation of daughter cells of different sizes rely on the correct orientation and position of the mitotic spindle. In the Drosophila embryo, the determinant Prospero is localized basally and is segregated equally to daughters of similar cell size during ep...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/71323

    authors： Kaltschmidt JA,Davidson CM,Brown NH,Brand AH

    更新日期：2000-01-01 00:00:00

  • The endogenous ligand Stunted of the GPCR Methuselah extends lifespan in Drosophila.

    abstract：:Many extracellular signals are transmitted to the interior of the cell by receptors with seven membrane-spanning helices that trigger their effects by means of heterotrimeric guanine-nucleotide-binding regulatory proteins (G proteins). These G-protein-coupled receptors (GPCRs) control various physiological functions i...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1133

    authors： Cvejic S,Zhu Z,Felice SJ,Berman Y,Huang XY

    更新日期：2004-06-01 00:00:00

  • Orchestrating Wnt signalling for metabolic liver zonation.

    abstract：:Wnt/β-catenin signalling is an important regulator of liver development, zonation and regeneration. The cell surface complex RSPO-LGR4/5-ZNF3/RNF43 is now shown to direct Wnt/β-catenin signalling in orchestrating the division of the liver into functionally distinct metabolic zones, providing insights into the mechanis...

    journal_title：Nature cell biology

    pub_type： 评论,杂志文章

    doi：10.1038/ncb3349

    authors： Birchmeier W

    更新日期：2016-04-27 00:00:00

  • The type I TGF-beta receptor engages TRAF6 to activate TAK1 in a receptor kinase-independent manner.

    abstract：:Transforming growth factor-beta (TGF-beta) is a multifunctional cytokine that regulates embryonic development and tissue homeostasis; however, aberrations of its activity occur in cancer. TGF-beta signals through its Type II and Type I receptors (TbetaRII and TbetaRI) causing phosphorylation of Smad proteins. TGF-beta...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1780

    authors： Sorrentino A,Thakur N,Grimsby S,Marcusson A,von Bulow V,Schuster N,Zhang S,Heldin CH,Landström M

    更新日期：2008-10-01 00:00:00