Abstract：
:The enteric nervous system (ENS) predominantly originates from vagal neural crest (VNC) cells that emerge from the caudal hindbrain, invade the foregut and populate the gastrointestinal tract. However, the gene regulatory network (GRN) orchestrating the early specification of VNC remains unknown. Using an EdnrB enhancer, we generated a comprehensive temporal map of the chromatin and transcriptional landscape of VNC in the avian model, revealing three VNC cell clusters (neural, neurogenic and mesenchymal), each predetermined epigenetically prior to neural tube delamination. We identify and functionally validate regulatory cores (Sox10/Tfap2B/SoxB/Hbox) mediating each programme and elucidate their combinatorial activities with other spatiotemporally specific transcription factors (bHLH/NR). Our global deconstruction of the VNC-GRN in vivo sheds light on critical early regulatory mechanisms that may influence the divergent neural phenotypes in enteric neuropathies.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Ling ITC,Sauka-Spengler Tdoi
10.1038/s41556-019-0428-9subject
Has Abstractpub_date
2019-12-01 00:00:00pages
1504-1517issue
12eissn
1465-7392issn
1476-4679pii
10.1038/s41556-019-0428-9journal_volume
21pub_type
杂志文章abstract：:The spindle assembly checkpoint (SAC) is a unique signalling mechanism that responds to the state of attachment of the kinetochore to spindle microtubules. SAC signalling is activated by unattached kinetochores, and it is silenced after these kinetochores form end-on microtubule attachments. Although the biochemical c...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3179
更新日期：2015-07-01 00:00:00
abstract：:Several subunits of the class III phosphatidylinositol-3-OH kinase (PI(3)K-III) complex are known as tumour suppressors. Here we uncover a function for this complex and its catalytic product phosphatidylinositol-3-phosphate (PtdIns(3)P) in cytokinesis. We show that PtdIns(3)P localizes to the midbody during cytokinesi...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2036
更新日期：2010-04-01 00:00:00
abstract：:Analysis of the mechanisms underlying cell fates requires the molecular quantification of cellular features. Classical techniques use population average readouts at single time points. However, these approaches mask cellular heterogeneity and dynamics and are limited for studying rare and heterogeneous cell population...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb3042
更新日期：2014-10-01 00:00:00
abstract：:The metazoan nuclear pore complex (NPC) disassembles during mitosis, and many of its constituents distribute onto spindles and kinetochores, including the Nup107-160 sub-complex. We have found that Nup107-160 interacts with the gamma-tubulin ring complex (gamma-TuRC), an essential and conserved microtubule nucleator, ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2016
更新日期：2010-02-01 00:00:00
abstract：:Neural Wiskott-Aldrich syndrome protein (N-WASP) and WAVE are members of a family of proteins that use the Arp2/3 complex to stimulate actin assembly in actin-based motile processes. By entering into distinct macromolecular complexes, they act as convergent nodes of different signalling pathways. The role of WAVE in g...
journal_title：Nature cell biology
pub_type： 信件
doi：10.1038/ncb1304
更新日期：2005-10-01 00:00:00
abstract：:Caenorhabditis elegans chromosomes contain specialized regions called pairing centres, which mediate homologous pairing and synapsis during meiosis. Four related proteins, ZIM-1, 2, 3 and HIM-8, associate with these sites and are required for their essential functions. Here we show that short sequence elements enriche...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1904
更新日期：2009-08-01 00:00:00
abstract：:Rad23 is a nucleotide-excision repair protein with a previously unknown biochemical function. We determined that yeast and human Rad23 inhibited multi-ubiquitin (Ub) chain formation and the degradation of proteolytic substrates. Significantly, Rad23 could be co-precipitated with a substrate that contained a short mult...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35023547
更新日期：2000-09-01 00:00:00
abstract：:The store-operated calcium channel (SOC) located in the plasma membrane (PM) mediates capacitative entry of extracellular calcium after depletion of intracellular calcium stores in the endoplasmic or sarcoplasmic reticulum (ER/SR). An intimate interaction between the PM and the ER/SR is essential for the operation of ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb788
更新日期：2002-05-01 00:00:00
abstract：:The physical characteristics of tumours are intricately linked to the tumour phenotype and difficulties during treatment. Many factors contribute to the increased stiffness of tumours; from increased matrix deposition, matrix remodelling by forces from cancer cells and stromal fibroblasts, matrix crosslinking, increas...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/s41556-018-0131-2
更新日期：2018-07-01 00:00:00
abstract：:Examination of the tumour suppressor adenomatous polyposis coli (APC) has shown that it may be multifunctional. Recent work has demonstrated dynamic interactions of APC with the microtubule cytoskeleton, supporting the idea that APC has an important function in cell migration. ...
journal_title：Nature cell biology
pub_type： 新闻,评审
doi：10.1038/35008685
更新日期：2000-04-01 00:00:00
abstract：:Localization and activation of heterotrimeric G proteins have a crucial role during asymmetric cell division. The asymmetric division of the Drosophila sensory precursor cell (pl) is polarized along the antero-posterior axis by Frizzled signalling and, during this division, activation of Galphai depends on Partner of ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1319
更新日期：2005-11-01 00:00:00
abstract：:Reference datasets should be accessible independently of scientific papers in a citable form, allowing attribution. ...
journal_title：Nature cell biology
pub_type： 社论
doi：10.1038/ncb1109-1273a
更新日期：2009-11-01 00:00:00
abstract：:The ATR checkpoint kinase coordinates cellular responses to DNA replication stress. Budding yeast contain three activators of Mec1 (the ATR orthologue); however, only TOPBP1 is known to activate ATR in vertebrates. We identified ETAA1 as a replication stress response protein in two proteomic screens. ETAA1-deficient c...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3415
更新日期：2016-11-01 00:00:00
abstract：:Organization of chromatin by epigenetic mechanisms is essential for establishing and maintaining cellular identity in developing and adult organisms. A key question that remains unresolved about this process is how epigenetic marks are transmitted to the next cell generation during cell division. Here we provide a mod...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1787
更新日期：2008-11-01 00:00:00
abstract：:Telomere attrition and other forms of telomere damage can activate the ATM kinase pathway. What generates the DNA damage signal at mammalian chromosome ends or at other double-strand breaks is not known. Telomere dysfunction is often accompanied by disappearance of the 3' telomeric overhang, raising the possibility th...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1275
更新日期：2005-07-01 00:00:00
abstract：:The anaphase-promoting complex or cyclosome (APC/C) initiates mitotic exit by ubiquitylating cell-cycle regulators such as cyclin B1 and securin. Lys 48-linked ubiquitin chains represent the canonical signal targeting proteins for degradation by the proteasome, but they are not required for the degradation of cyclin B...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2425
更新日期：2012-01-29 00:00:00
abstract：:Innovations in live-cell microscopy and single-molecule analysis have allowed a new direct view of nuclear messenger RNA dynamics. A new study extends previous analyses of mRNA-protein intranuclear transport and links this critical step to the kinetics of moving through nuclear pore complexes. Seeing nuclear mRNA on t...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb0610-525
更新日期：2010-06-01 00:00:00
abstract：:Protein substrates of the proteasome must apparently be unfolded and translocated through a narrow channel to gain access to the proteolytic active sites of the enzyme. Protein folding in vivo is mediated by molecular chaperones. Here, to test for chaperone activity of the proteasome, we assay the reactivation of dena...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/12043
更新日期：1999-08-01 00:00:00
abstract：:The stability and membrane localization of the transforming growth factor-β (TGF-β) type I receptor (TβRI) determines the levels of TGF-β signalling. TβRI is targeted for ubiquitylation-mediated degradation by the SMAD7-SMURF2 complex. Here we performed a genome-wide gain-of-function screen and identified ubiquitin-sp...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2522
更新日期：2012-06-17 00:00:00
abstract：:The actin cable is a supracellular structure that embryonic epithelia produce to close gaps. However, the action of the cable remains debated. Here, we address the function of the cable using Drosophila dorsal closure, a paradigm to understand wound healing. First, we show that the actin cytoskeleton protein Zasp52 is...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3421
更新日期：2016-11-01 00:00:00
abstract：:In Huntington disease, polyglutamine expansion of the protein huntingtin (Htt) leads to selective neurodegenerative loss of medium spiny neurons throughout the striatum by an unknown apoptotic mechanism. Binding of Hip-1, a protein normally associated with Htt, is reduced by polyglutamine expansion. Free Hip-1 binds t...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb735
更新日期：2002-02-01 00:00:00
abstract：:Aurora A, an integral mitotic kinase, is essential for microtubule dynamics of post-mitotic neurons. PKCzeta activates Aurora A, which in turn phosphorylates NDEL1 to promote neurite extension. This raises the possibility that Aurora A may also be involved in establishing cell polarity and axon/dendrite elaboration in...
journal_title：Nature cell biology
pub_type： 评论,新闻
doi：10.1038/ncb0909-1053
更新日期：2009-09-01 00:00:00
abstract：:Pyruvate kinase M2 (PKM2) is upregulated in multiple cancer types and contributes to the Warburg effect by unclear mechanisms. Here we demonstrate that EGFR-activated ERK2 binds directly to PKM2 Ile 429/Leu 431 through the ERK2 docking groove and phosphorylates PKM2 at Ser 37, but does not phosphorylate PKM1. Phosphor...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2629
更新日期：2012-12-01 00:00:00
abstract：:Chromatin structure is thought to play a critical role in gene expression. Using the lac operator/repressor system and two colour variants of green fluorescent protein (GFP), we developed a system to visualize a gene and its protein product directly in living cells, allowing us to examine the spatial organization and ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35046510
更新日期：2000-12-01 00:00:00
abstract：:During meiosis, homologous chromosomes are sorted into pairs and are then intimately aligned, or synapsed, along their lengths while a proteinaceous structure, the synaptonemal complex, is assembled between them. However, little is known about how chromosomes first recognise each other. Here we show, by comparing the ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1168
更新日期：2004-09-01 00:00:00
abstract：:The mitochondrial inner membrane is the central energy-converting membrane of eukaryotic cells. The electrochemical proton gradient generated by the respiratory chain drives the ATP synthase. To maintain this proton-motive force, the inner membrane forms a tight barrier and strictly controls the translocation of ions....
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1635
更新日期：2007-10-01 00:00:00
abstract：:Apoptosis is induced by caspases, which are members of the cysteine protease family. Caspases are synthesized as inactive zymogens and initiator caspases first gain activity by associating with an oligomeric complex of their adaptor proteins, such as the apoptosome. Activated initiator caspases subsequently cleave and...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1803
更新日期：2008-12-01 00:00:00
abstract：:Stat3 has been shown to regulate lysosome membrane permeabilization and cell death in vivo during post-lactation mammary gland involution. It is now revealed that Stat3 induces lysosome membrane permeabilization by causing phagocytosis of milk fat globules, which destabilize the lysosome membrane leading to leakage of...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb3059
更新日期：2014-11-01 00:00:00
abstract：:Over their lifetime, long-term haematopoietic stem cells (HSC) are exposed to a variety of stress conditions that they must endure. Many stresses, such as infection/inflammation, reactive oxygen species, nutritional deprivation and hypoxia, activate unfolded protein response signalling, which induces either adaptive c...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-019-0285-6
更新日期：2019-03-01 00:00:00
abstract：:Influx of Ca(2+) through store-operated Ca(2+) channels (SOCs) is a central component of receptor-evoked Ca(2+) signals. Orai channels are SOCs that are gated by STIM1, a Ca(2+) sensor located in the ER but how it gates and regulates the Orai channels is unknown. Here, we report the molecular basis for gating of Orais...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1842
更新日期：2009-03-01 00:00:00
