Abstract：
:Apoptosis is induced by caspases, which are members of the cysteine protease family. Caspases are synthesized as inactive zymogens and initiator caspases first gain activity by associating with an oligomeric complex of their adaptor proteins, such as the apoptosome. Activated initiator caspases subsequently cleave and activate effector caspases. Although such a proteolytic cascade would predict that a small number of active caspases could irreversibly amplify caspase activity and trigger apoptosis, many cells can maintain moderate levels of caspase activity to perform non-apoptotic roles in cellular differentiation, shape change and migration. Here we show that the Drosophila melanogaster apoptosome engages in a feedback inhibitory loop, which moderates its activation level in vivo. Specifically, the adaptor protein Apaf-1 lowers the level of its associated initiator caspase Dronc, without triggering apoptosis. Conversely, Dronc lowers Apaf-1 protein levels. This mutual suppression depends on the catalytic site of Dronc and a caspase cleavage site within Apaf-1. Moreover, the Drosophila inhibitor of apoptosis protein 1 (Diap1) is required for this process. We speculate that this feedback inhibition allows cells to regulate the degree of caspase activation for apoptotic and non-apoptotic purposes.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Shapiro PJ,Hsu HH,Jung H,Robbins ES,Ryoo HDdoi
10.1038/ncb1803subject
Has Abstractpub_date
2008-12-01 00:00:00pages
1440-6issue
12eissn
1465-7392issn
1476-4679pii
ncb1803journal_volume
10pub_type
杂志文章abstract：:The target of rapamycin complex 2 (TORC2) plays a key role in maintaining the homeostasis of plasma membrane (PM) tension. TORC2 activation following increased PM tension involves redistribution of the Slm1 and 2 paralogues from PM invaginations known as eisosomes into membrane compartments containing TORC2. How Slm1/...
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pub_type： 杂志文章
doi：10.1038/s41556-018-0150-z
更新日期：2018-09-01 00:00:00
abstract：:In three-dimensional matrices cancer cells move with a rounded, amoeboid morphology that is controlled by ROCK-dependent contraction of acto-myosin. In this study, we show that PDK1 is required for phosphorylation of myosin light chain and cell motility, both on deformable gels and in vivo. Depletion of PDK1 alters th...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1675
更新日期：2008-02-01 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1140
更新日期：2004-07-01 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2328
更新日期：2011-09-18 00:00:00
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pub_type： 杂志文章
doi：10.1038/ncb1769
更新日期：2008-09-01 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2633
更新日期：2013-01-01 00:00:00
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pub_type： 评论,杂志文章
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更新日期：2009-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1038/ncb1001-891
更新日期：2001-10-01 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-018-0192-2
更新日期：2018-10-01 00:00:00
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pub_type： 杂志文章,已发布勘误
doi：10.1038/s41556-018-0053-z
更新日期：2018-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1038/ncb1573
更新日期：2007-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1038/ncb956
更新日期：2003-04-01 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35010558
更新日期：2000-05-01 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35036326
更新日期：2000-10-01 00:00:00
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journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb1008-1131
更新日期：2008-10-01 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3573
更新日期：2017-08-01 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3111
更新日期：2015-04-01 00:00:00
abstract：:The heterogeneity of exosomal populations has hindered our understanding of their biogenesis, molecular composition, biodistribution and functions. By employing asymmetric flow field-flow fractionation (AF4), we identified two exosome subpopulations (large exosome vesicles, Exo-L, 90-120 nm; small exosome vesicles, Ex...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-018-0040-4
更新日期：2018-03-01 00:00:00
abstract：:During apoptosis, dying cells are swiftly removed by phagocytes. It is not fully understood how apoptotic cells are recognized by phagocytes. Here we report the identification and characterization of the Caenorhabditis elegans ttr-52 gene, which encodes a transthyretin-like protein and is required for efficient cell c...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2068
更新日期：2010-07-01 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2756
更新日期：2013-06-01 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1275
更新日期：2005-07-01 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3390
更新日期：2016-08-01 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1278
更新日期：2005-08-01 00:00:00
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journal_title：Nature cell biology
pub_type：
doi：10.1038/ncb1004-923
更新日期：2004-10-01 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3369
更新日期：2016-07-01 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2139
更新日期：2011-01-01 00:00:00
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journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb3197
更新日期：2015-07-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature cell biology
pub_type： 杂志文章,已发布勘误
doi：10.1038/s41556-020-0479-y
更新日期：2020-03-01 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3251
更新日期：2015-11-01 00:00:00
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journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1194
更新日期：2004-12-01 00:00:00
