当前位置： SCI文献检索 > NATURE CELL BIOLOGY期刊下所有文献 > CDK1-dependent phosphorylation of EZH2 suppresses methylation of H3K27 and promotes osteogenic differentiation of human mesenchymal stem cells.

CDK1-dependent phosphorylation of EZH2 suppresses methylation of H3K27 and promotes osteogenic differentiation of human mesenchymal stem cells.

Abstract：

:Enhancer of zeste homologue 2 (EZH2) is the catalytic subunit of Polycomb repressive complex 2 (PRC2) and catalyses the trimethylation of histone H3 on Lys 27 (H3K27), which represses gene transcription. EZH2 enhances cancer-cell invasiveness and regulates stem cell differentiation. Here, we demonstrate that EZH2 can be phosphorylated at Thr 487 through activation of cyclin-dependent kinase 1 (CDK1). The phosphorylation of EZH2 at Thr 487 disrupted EZH2 binding with the other PRC2 components SUZ12 and EED, and thereby inhibited EZH2 methyltransferase activity, resulting in inhibition of cancer-cell invasion. In human mesenchymal stem cells, activation of CDK1 promoted mesenchymal stem cell differentiation into osteoblasts through phosphorylation of EZH2 at Thr 487. These findings define a signalling link between CDK1 and EZH2 that may have an important role in diverse biological processes, including cancer-cell invasion and osteogenic differentiation of mesenchymal stem cells.

journal_name

Nat Cell Biol

journal_title

Nature cell biology

authors

Wei Y,Chen YH,Li LY,Lang J,Yeh SP,Shi B,Yang CC,Yang JY,Lin CY,Lai CC,Hung MC

doi

10.1038/ncb2139

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

87-94

issue

1

eissn

1465-7392

issn

1476-4679

pii

ncb2139

journal_volume

13

pub_type

杂志文章

相关文献

NATURE CELL BIOLOGY文献大全
  • EZH2 promotes degradation of stalled replication forks by recruiting MUS81 through histone H3 trimethylation.

    abstract：:The emergence of resistance to poly-ADP-ribose polymerase inhibitors (PARPi) poses a threat to the treatment of BRCA1 and BRCA2 (BRCA1/2)-deficient tumours. Stabilization of stalled DNA replication forks is a recently identified PARPi-resistance mechanism that promotes genomic stability in BRCA1/2-deficient cancers. D...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb3626

    authors： Rondinelli B,Gogola E,Yücel H,Duarte AA,van de Ven M,van der Sluijs R,Konstantinopoulos PA,Jonkers J,Ceccaldi R,Rottenberg S,D'Andrea AD

    更新日期：2017-11-01 00:00:00

  • Linking HSCs to their youth.

    abstract：:Fetal haematopoietic stem cells (HSCs) self-renew extensively to build the blood system from scratch. The protein Lin28b negatively regulates the microRNA let-7 to keep levels of its target Hmga2 high, hence conferring high self-renewal potential to fetal HSCs. This regulatory circuit can be experimentally modulated t...

    journal_title：Nature cell biology

    pub_type： 评论,新闻

    doi：10.1038/ncb2817

    authors： Pietras EM,Passegué E

    更新日期：2013-08-01 00:00:00

  • Adherens junction treadmilling during collective migration.

    abstract：:Collective cell migration is essential for both physiological and pathological processes. Adherens junctions (AJs) maintain the integrity of the migrating cell group and promote cell coordination while allowing cellular rearrangements. Here, we show that AJs undergo a continuous treadmilling along the lateral sides of...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb2985

    authors： Peglion F,Llense F,Etienne-Manneville S

    更新日期：2014-07-01 00:00:00

  • Cdc42-MRCK and Rho-ROCK signalling cooperate in myosin phosphorylation and cell invasion.

    abstract：:Actomyosin contractility is a mechanism by which cells exert locomotory force against their environment. Signalling downstream of the small GTPase Rho increases contractility through Rho-kinase (ROCK)-mediated regulation of myosin-II light chain (MLC2) phosphorylation. Cdc42 signalling has been shown to control cell p...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1230

    authors： Wilkinson S,Paterson HF,Marshall CJ

    更新日期：2005-03-01 00:00:00

  • Erythromyeloid progenitors give rise to a population of osteoclasts that contribute to bone homeostasis and repair.

    abstract：:Osteoclasts are multinucleated cells of the monocyte/macrophage lineage that degrade bone. Here, we used lineage tracing studies-labelling cells expressing Cx3cr1, Csf1r or Flt3-to identify the precursors of osteoclasts in mice. We identified an erythromyeloid progenitor (EMP)-derived osteoclast precursor population. ...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/s41556-019-0437-8

    authors： Yahara Y,Barrientos T,Tang YJ,Puviindran V,Nadesan P,Zhang H,Gibson JR,Gregory SG,Diao Y,Xiang Y,Qadri YJ,Souma T,Shinohara ML,Alman BA

    更新日期：2020-01-01 00:00:00

  • Lysosomal positioning coordinates cellular nutrient responses.

    abstract：:mTOR (mammalian target of rapamycin) signalling and macroautophagy (henceforth autophagy) regulate numerous pathological and physiological processes, including cellular responses to altered nutrient levels. However, the mechanisms regulating mTOR and autophagy remain incompletely understood. Lysosomes are dynamic intr...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb2204

    authors： Korolchuk VI,Saiki S,Lichtenberg M,Siddiqi FH,Roberts EA,Imarisio S,Jahreiss L,Sarkar S,Futter M,Menzies FM,O'Kane CJ,Deretic V,Rubinsztein DC

    更新日期：2011-04-01 00:00:00

  • The kinetochore encodes a mechanical switch to disrupt spindle assembly checkpoint signalling.

    abstract：:The spindle assembly checkpoint (SAC) is a unique signalling mechanism that responds to the state of attachment of the kinetochore to spindle microtubules. SAC signalling is activated by unattached kinetochores, and it is silenced after these kinetochores form end-on microtubule attachments. Although the biochemical c...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb3179

    authors： Aravamudhan P,Goldfarb AA,Joglekar AP

    更新日期：2015-07-01 00:00:00

  • Identification of stem cells that maintain and regenerate lingual keratinized epithelial cells.

    abstract：:Lingual keratinized epithelial cells, which constitute the filiform papillae of the tongue, have one of the most rapid tissue turnover rates in the mammalian body and are thought to be the source of squamous cell carcinoma of the tongue. However, the mechanism of tissue maintenance and regeneration is largely unknown ...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb2719

    authors： Tanaka T,Komai Y,Tokuyama Y,Yanai H,Ohe S,Okazaki K,Ueno H

    更新日期：2013-05-01 00:00:00

  • Noelin-1 is a secreted glycoprotein involved in generation of the neural crest.

    abstract：:The vertebrate neural crest arises at the border of the neural plate during early stages of nervous system development; however, little is known about the molecular mechanisms underlying neural crest formation. Here we identify a secreted protein, Noelin-1, which has the ability to prolong neural crest production. Noe...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/35008643

    authors： Barembaum M,Moreno TA,LaBonne C,Sechrist J,Bronner-Fraser M

    更新日期：2000-04-01 00:00:00

  • A potential link between obesity and neural stem cell dysfunction.

    abstract：:Given the important role of the hypothalamus in regulating feeding and metabolism, there has been considerable interest in a possible function for hypothalamic stem cells in modulating body weight in health and disease. Mice given a high-fat diet develop inflammation in the hypothalamus and lose key types of neurons. ...

    journal_title：Nature cell biology

    pub_type： 新闻

    doi：10.1038/ncb2599

    authors： Livesey FJ

    更新日期：2012-10-01 00:00:00

  • Differential function of Tie2 at cell-cell contacts and cell-substratum contacts regulated by angiopoietin-1.

    abstract：:Tie2 belongs to the receptor tyrosine kinase family and functions as a receptor for Angiopoietin-1 (Ang1). Gene-targeting analyses of either Ang1 or Tie2 in mice reveal a critical role of Ang1-Tie2 signalling in developmental vascular formation. It remains elusive how the Tie2 signalling pathway plays distinct roles i...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1714

    authors： Fukuhara S,Sako K,Minami T,Noda K,Kim HZ,Kodama T,Shibuya M,Takakura N,Koh GY,Mochizuki N

    更新日期：2008-05-01 00:00:00

  • Chromosomes at loose ends.

    abstract：:Broken ends of a budding yeast chromosome exhibit increased mobility, presumably to facilitate repair by recombination. A new study reports that increased mobility reflects the untethering of the broken chromosome, triggered by a DNA damage response that phosphorylates the Cep3 kinetochore protein and weakens the asso...

    journal_title：Nature cell biology

    pub_type： 评论,杂志文章

    doi：10.1038/ncb3321

    authors： Nakajima Y,Haber JE

    更新日期：2016-03-01 00:00:00

  • A tension-mediated glycocalyx-integrin feedback loop promotes mesenchymal-like glioblastoma.

    abstract：:Glioblastoma multiforme (GBMs) are recurrent lethal brain tumours. Recurrent GBMs often exhibit mesenchymal, stem-like phenotypes that could explain their resistance to therapy. Analyses revealed that recurrent GBMs have increased tension and express high levels of glycoproteins that increase the bulkiness of the glyc...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/s41556-018-0183-3

    authors： Barnes JM,Kaushik S,Bainer RO,Sa JK,Woods EC,Kai F,Przybyla L,Lee M,Lee HW,Tung JC,Maller O,Barrett AS,Lu KV,Lakins JN,Hansen KC,Obernier K,Alvarez-Buylla A,Bergers G,Phillips JJ,Nam DH,Bertozzi CR,Weaver VM

    更新日期：2018-10-01 00:00:00

  • Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation.

    abstract：:The heterogeneity of exosomal populations has hindered our understanding of their biogenesis, molecular composition, biodistribution and functions. By employing asymmetric flow field-flow fractionation (AF4), we identified two exosome subpopulations (large exosome vesicles, Exo-L, 90-120 nm; small exosome vesicles, Ex...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/s41556-018-0040-4

    authors： Zhang H,Freitas D,Kim HS,Fabijanic K,Li Z,Chen H,Mark MT,Molina H,Martin AB,Bojmar L,Fang J,Rampersaud S,Hoshino A,Matei I,Kenific CM,Nakajima M,Mutvei AP,Sansone P,Buehring W,Wang H,Jimenez JP,Cohen-Gould L,P

    更新日期：2018-03-01 00:00:00

  • CRISPR mutagenesis screening of mice.

    abstract：: ...

    journal_title：Nature cell biology

    pub_type： 评论,杂志文章

    doi：10.1038/s41556-018-0224-y

    authors： Serebrenik YV,Shalem O

    更新日期：2018-11-01 00:00:00

  • ARF mediates recruitment of PtdIns-4-OH kinase-beta and stimulates synthesis of PtdIns(4,5)P2 on the Golgi complex.

    abstract：:The small GTPase ADP-ribosylation factor (ARF) regulates the structure and function of the Golgi complex through mechanisms that are understood only in part, and which include an ability to control the assembly of coat complexes and phospholipase D (PLD). Here we describe a new property of ARF, the ability to recruit ...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/12993

    authors： Godi A,Pertile P,Meyers R,Marra P,Di Tullio G,Iurisci C,Luini A,Corda D,De Matteis MA

    更新日期：1999-09-01 00:00:00

  • SADB phosphorylation of gamma-tubulin regulates centrosome duplication.

    abstract：:Symmetrical cell division requires duplication of DNA and protein content to generate two daughter cells. Centrosomes also duplicate during cell division, but the mechanism controlling this process is incompletely understood. We describe an alternative splice form of SadB encoding a short SADB Ser/Thr kinase whose act...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1921

    authors： Alvarado-Kristensson M,Rodríguez MJ,Silió V,Valpuesta JM,Carrera AC

    更新日期：2009-09-01 00:00:00

  • A nutrient-induced affinity switch controls mTORC1 activation by its Rag GTPase-Ragulator lysosomal scaffold.

    abstract：:A key step in nutrient sensing is activation of the master growth regulator, mTORC1 kinase, on the lysosomal membrane. Nutrients enable mTORC1 scaffolding by a complex composed of the Rag GTPases (Rags) and Ragulator, but the underlying mechanism of mTORC1 capture is poorly understood. Combining dynamic imaging in cel...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/s41556-018-0148-6

    authors： Lawrence RE,Cho KF,Rappold R,Thrun A,Tofaute M,Kim DJ,Moldavski O,Hurley JH,Zoncu R

    更新日期：2018-09-01 00:00:00

  • Control of metastatic progression by microRNA regulatory networks.

    abstract：:Aberrant microRNA (miRNA) expression is a defining feature of human malignancy. Specific miRNAs have been identified as promoters or suppressors of metastatic progression. miRNAs control metastasis through divergent or convergent regulation of metastatic gene pathways. Some miRNA regulatory networks govern cell-autono...

    journal_title：Nature cell biology

    pub_type： 杂志文章,评审

    doi：10.1038/ncb2769

    authors： Pencheva N,Tavazoie SF

    更新日期：2013-06-01 00:00:00

  • Vesicles modulate an actin network for asymmetric spindle positioning.

    abstract：:Actin networks drive many essential cellular processes, including cell migration, cytokinesis and tissue morphogenesis. However, how cells organize and regulate dynamic actin networks that consist of long, unbranched actin filaments is only poorly understood. This study in mouse oocytes reveals that cells can use vesi...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb2802

    authors： Holubcová Z,Howard G,Schuh M

    更新日期：2013-08-01 00:00:00

  • RNA takes over control of DNA break repair.

    abstract：:Small RNAs generated at DNA break sites are implicated in mammalian DNA repair. Now, a study shows that following the formation of DNA double-strand breaks, bidirectional transcription events adjacent to the break generate small RNAs that trigger the DNA damage response by local RNA:RNA interactions. ...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb3645

    authors： Storici F,Tichon AE

    更新日期：2017-11-29 00:00:00

  • Cell biology in the Antarctic: studying life in the freezer.

    abstract：:Many classical biologists working in Antarctica have 'gone molecular' to study the physiological basis for life on or below the ice. Investigating the remarkable adaptations of specific cells, organelles and molecules to this extreme environment can provide new perspectives on the processes studied in conventional exp...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb0103-3

    authors： Römisch K,Matheson T

    更新日期：2003-01-01 00:00:00

  • TGF-beta-mediated phosphorylation of hnRNP E1 induces EMT via transcript-selective translational induction of Dab2 and ILEI.

    abstract：:Transforming growth factor-beta (TGF-beta) induces epithelial-mesenchymal transdifferentiation (EMT) accompanied by cellular differentiation and migration. Despite extensive transcriptomic profiling, the identification of TGF-beta-inducible, EMT-specific genes has met with limited success. Here we identify a post-tran...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb2029

    authors： Chaudhury A,Hussey GS,Ray PS,Jin G,Fox PL,Howe PH

    更新日期：2010-03-01 00:00:00

  • Autophagosome-lysosome fusion triggers a lysosomal response mediated by TLR9 and controlled by OCRL.

    abstract：:Phosphoinositides (PtdIns) control fundamental cell processes, and inherited defects of PtdIns kinases or phosphatases cause severe human diseases, including Lowe syndrome due to mutations in OCRL, which encodes a PtdIns(4,5)P2 5-phosphatase. Here we unveil a lysosomal response to the arrival of autophagosomal cargo i...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb3386

    authors： De Leo MG,Staiano L,Vicinanza M,Luciani A,Carissimo A,Mutarelli M,Di Campli A,Polishchuk E,Di Tullio G,Morra V,Levtchenko E,Oltrabella F,Starborg T,Santoro M,Di Bernardo D,Devuyst O,Lowe M,Medina DL,Ballabio A,De Ma

    更新日期：2016-08-01 00:00:00

  • FAPPs control Golgi-to-cell-surface membrane traffic by binding to ARF and PtdIns(4)P.

    abstract：:The molecular mechanisms underlying the formation of carriers trafficking from the Golgi complex to the cell surface are still ill-defined; nevertheless, the involvement of a lipid-based machinery is well established. This includes phosphatidylinositol 4-phosphate (PtdIns(4)P), the precursor for phosphatidylinositol 4...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb1119

    authors： Godi A,Di Campli A,Konstantakopoulos A,Di Tullio G,Alessi DR,Kular GS,Daniele T,Marra P,Lucocq JM,De Matteis MA

    更新日期：2004-05-01 00:00:00

  • PP2A activates brassinosteroid-responsive gene expression and plant growth by dephosphorylating BZR1.

    abstract：:When brassinosteroid levels are low, the GSK3-like kinase BIN2 phosphorylates and inactivates the BZR1 transcription factor to inhibit growth in plants. Brassinosteroid promotes growth by inducing dephosphorylation of BZR1, but the phosphatase that dephosphorylates BZR1 has remained unknown. Here, using tandem affinit...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb2151

    authors： Tang W,Yuan M,Wang R,Yang Y,Wang C,Oses-Prieto JA,Kim TW,Zhou HW,Deng Z,Gampala SS,Gendron JM,Jonassen EM,Lillo C,DeLong A,Burlingame AL,Sun Y,Wang ZY

    更新日期：2011-02-01 00:00:00

  • Threonine 68 is required for radiation-induced phosphorylation and activation of Cds1.

    abstract：:In response to DNA damage, eukaryotic cells use a system of checkpoint controls to delay cell-cycle progression. Checkpoint delays provide time for repair of damaged DNA before its replication in S phase and before segregation of chromatids in M phase. The Cds1 (Chk2) tumour-suppressor protein has been implicated in c...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/35036406

    authors： Melchionna R,Chen XB,Blasina A,McGowan CH

    更新日期：2000-10-01 00:00:00

  • Dissecting the link between stress fibres and focal adhesions by CALI with EGFP fusion proteins.

    abstract：:Chromophore-assisted laser inactivation (CALI) is a light-mediated technique used to selectively inactivate proteins within cells. Here, we demonstrate that GFP can be used as a CALI reagent to locally inactivate proteins in living cells. We show that focused laser irradiation of EGFP-alpha-actinin expressed in Swiss ...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb772

    authors： Rajfur Z,Roy P,Otey C,Romer L,Jacobson K

    更新日期：2002-04-01 00:00:00

  • Breast-cancer-secreted miR-122 reprograms glucose metabolism in premetastatic niche to promote metastasis.

    abstract：:Reprogrammed glucose metabolism as a result of increased glycolysis and glucose uptake is a hallmark of cancer. Here we show that cancer cells can suppress glucose uptake by non-tumour cells in the premetastatic niche, by secreting vesicles that carry high levels of the miR-122 microRNA. High miR-122 levels in the cir...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb3094

    authors： Fong MY,Zhou W,Liu L,Alontaga AY,Chandra M,Ashby J,Chow A,O'Connor ST,Li S,Chin AR,Somlo G,Palomares M,Li Z,Tremblay JR,Tsuyada A,Sun G,Reid MA,Wu X,Swiderski P,Ren X,Shi Y,Kong M,Zhong W,Chen Y,Wang SE

    更新日期：2015-02-01 00:00:00

  • Protein kinase C controls lysosome biogenesis independently of mTORC1.

    abstract：:Lysosomes respond to environmental cues by controlling their own biogenesis, but the underlying mechanisms are poorly understood. Here we describe a protein kinase C (PKC)-dependent and mTORC1-independent mechanism for regulating lysosome biogenesis, which provides insights into previously reported effects of PKC on l...

    journal_title：Nature cell biology

    pub_type： 杂志文章

    doi：10.1038/ncb3407

    authors： Li Y,Xu M,Ding X,Yan C,Song Z,Chen L,Huang X,Wang X,Jian Y,Tang G,Tang C,Di Y,Mu S,Liu X,Liu K,Li T,Wang Y,Miao L,Guo W,Hao X,Yang C

    更新日期：2016-10-01 00:00:00