Abstract:
:The small GTPase ADP-ribosylation factor (ARF) regulates the structure and function of the Golgi complex through mechanisms that are understood only in part, and which include an ability to control the assembly of coat complexes and phospholipase D (PLD). Here we describe a new property of ARF, the ability to recruit phosphatidylinositol-4-OH kinase-beta and a still unidentified phosphatidylinositol-4-phosphate-5-OH kinase to the Golgi complex, resulting in a potent stimulation of synthesis of phosphatidylinositol-4-phosphate and phosphatidylinositol-4,5-bisphosphate; this ability is independent of its activities on coat proteins and PLD. Phosphatidylinositol-4-OH kinase-beta is required for the structural integrity of the Golgi complex: transfection of a dominant-negative mutant of the kinase markedly alters the organization of the organelle.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Godi A,Pertile P,Meyers R,Marra P,Di Tullio G,Iurisci C,Luini A,Corda D,De Matteis MAdoi
10.1038/12993keywords:
subject
Has Abstractpub_date
1999-09-01 00:00:00pages
280-7issue
5eissn
1465-7392issn
1476-4679journal_volume
1pub_type
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