Abstract:
:Innovations in live-cell microscopy and single-molecule analysis have allowed a new direct view of nuclear messenger RNA dynamics. A new study extends previous analyses of mRNA-protein intranuclear transport and links this critical step to the kinetics of moving through nuclear pore complexes. Seeing nuclear mRNA on the move will impact future work on pore translocation and nuclear organization.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Noble KN,Wente SRdoi
10.1038/ncb0610-525subject
Has Abstractpub_date
2010-06-01 00:00:00pages
525-7issue
6eissn
1465-7392issn
1476-4679pii
ncb0610-525journal_volume
12pub_type
评论,杂志文章abstract::When co-translationally inserted into endoplasmic reticulum (ER) membranes, newly synthesized proteins encounter the lumenal environment of the ER, which contains chaperone proteins that facilitate the folding reactions necessary for protein oligomerization, maturation and export from the ER. Here we show, using a tem...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35010558
更新日期:2000-05-01 00:00:00
abstract::Haematopoietic stem cells (HSCs) arise in the developing aorta during embryogenesis. The number of HSC clones born has been estimated through transplantation, but experimental approaches to assess the absolute number of forming HSCs in a native setting have remained challenging. Here, we applied single-cell and clonal...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3444
更新日期:2017-01-01 00:00:00
abstract::Nucleocytoplasmic transport occurs through nuclear pore complexes (NPCs) embedded in the nuclear envelope. Here, we discovered an unexpected role for yeast dynein light chain (Dyn2) in the NPC. Dyn2 is a previously undescribed nucleoporin that functions as molecular glue to dimerize and stabilize the Nup82-Nsp1-Nup159...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1604
更新日期:2007-07-01 00:00:00
abstract::The ATR checkpoint kinase coordinates cellular responses to DNA replication stress. Budding yeast contain three activators of Mec1 (the ATR orthologue); however, only TOPBP1 is known to activate ATR in vertebrates. We identified ETAA1 as a replication stress response protein in two proteomic screens. ETAA1-deficient c...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3415
更新日期:2016-11-01 00:00:00
abstract::The transition from cellular quiescence (G0) into S phase is regulated by the mitogenic-activation of D-type cyclins and cyclin-dependent kinases (Cdks), the sequestration of the Cdk inhibitors (CDKIs), p21 and p27, and the hyperphosphorylation of Rb with release of E2F transcription factors. However, fibroblasts that...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1548
更新日期:2007-03-01 00:00:00
abstract::During the development of multicellular organisms, concerted actions of molecular signalling networks determine whether cells undergo proliferation, differentiation, death or ageing. Here we show that genetic inactivation of the stress signalling kinase, MKK7, a direct activator of JNKs in mice, results in embryonic l...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1098
更新日期:2004-03-01 00:00:00
abstract::Inactivation of the von Hippel-Lindau (VHL) tumour-suppressor protein (pVHL) is associated with the von Hippel-Lindau cancer syndrome and the majority of kidney cancers. New evidence suggests that pVHL has properties of an F-box protein that targets the alpha-subunits of hypoxia-inducible factor (HIF)-1 for oxygen-dep...
journal_title:Nature cell biology
pub_type: 评论,新闻
doi:10.1038/35017129
更新日期:2000-07-01 00:00:00
abstract::In vertebrates, centromeres lack defined sequences and are thought to be propagated by epigenetic mechanisms involving the incorporation of specialized nucleosomes containing the histone H3 variant centromere protein (CENP)-A. However, the precise mechanisms that target CENP-A to centromeres remain poorly understood. ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1396
更新日期:2006-05-01 00:00:00
abstract::The mitochondrial inner membrane is the central energy-converting membrane of eukaryotic cells. The electrochemical proton gradient generated by the respiratory chain drives the ATP synthase. To maintain this proton-motive force, the inner membrane forms a tight barrier and strictly controls the translocation of ions....
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1635
更新日期:2007-10-01 00:00:00
abstract::Transforming growth factor-beta (TGF-beta) induces epithelial-mesenchymal transdifferentiation (EMT) accompanied by cellular differentiation and migration. Despite extensive transcriptomic profiling, the identification of TGF-beta-inducible, EMT-specific genes has met with limited success. Here we identify a post-tran...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2029
更新日期:2010-03-01 00:00:00
abstract::Actin and myosin are major components of the cell cytoskeleton, with structural and regulatory functions that affect many essential cellular processes. Although they were traditionally thought to function only in the cytoplasm, it is now well accepted that actin and multiple myosins are found in the nucleus. Increasin...
journal_title:Nature cell biology
pub_type: 杂志文章,评审
doi:10.1038/ncb2364
更新日期:2011-11-02 00:00:00
abstract::Cell division is fundamental for all organisms. Here we report a genome-scale RNA-mediated interference screen in HeLa cells designed to identify human genes that are important for cell division. We have used a library of endoribonuclease-prepared short interfering RNAs for gene silencing and have used DNA content ana...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1659
更新日期:2007-12-01 00:00:00
abstract::Extracellular signal regulated kinases (ERKs) represent a signalling hub in many physiological responses and have pivotal functions in cell proliferation, differentiation, development and death, as well as in synaptic plasticity. Mitogen-activated protein kinase phosphatases (MKPs) selectively inactivate ERKs by depho...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1447
更新日期:2006-08-01 00:00:00
abstract::Phosphatase and tensin homologue (Pten) suppresses neoplastic growth by negatively regulating PI(3)K signalling through its phosphatase activity. To gain insight into the actions of non-catalytic Pten domains in normal physiological processes and tumorigenesis, we engineered mice lacking the PDZ-binding domain (PDZ-BD...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3369
更新日期:2016-07-01 00:00:00
abstract::Glioblastoma multiforme (GBMs) are recurrent lethal brain tumours. Recurrent GBMs often exhibit mesenchymal, stem-like phenotypes that could explain their resistance to therapy. Analyses revealed that recurrent GBMs have increased tension and express high levels of glycoproteins that increase the bulkiness of the glyc...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/s41556-018-0183-3
更新日期:2018-10-01 00:00:00
abstract::Loss-of-function (LOF) mutations in the endothelial cell (EC)-enriched gene endoglin (ENG) cause the human disease hereditary haemorrhagic telangiectasia-1, characterized by vascular malformations promoted by vascular endothelial growth factor A (VEGFA). How ENG deficiency alters EC behaviour to trigger these anomalie...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3534
更新日期:2017-06-01 00:00:00
abstract::The mitogen-activated protein kinase (MAPK) network is a conserved signalling module that regulates cell fate by transducing a myriad of growth-factor signals. The ability of this network to coordinate and process a variety of inputs from different growth-factor receptors into specific biological responses is, however...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1543
更新日期:2007-03-01 00:00:00
abstract::Cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-activated chloride channel expressed in a wide variety of epithelial cells, mutations of which are responsible for the hallmark defective chloride secretion observed in cystic fibrosis (CF). Although CFTR has been implicated in bicarbonate secretion,...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1047
更新日期:2003-10-01 00:00:00
abstract::The actin cable is a supracellular structure that embryonic epithelia produce to close gaps. However, the action of the cable remains debated. Here, we address the function of the cable using Drosophila dorsal closure, a paradigm to understand wound healing. First, we show that the actin cytoskeleton protein Zasp52 is...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3421
更新日期:2016-11-01 00:00:00
abstract::Canonical Wnt signalling has been implicated in mouse and human embryonic stem cell (ESC) maintenance; however, its requirement is controversial. β-catenin is the key component in this highly conserved Wnt pathway, acting as a transcriptional transactivator. However, β-catenin has additional roles at the plasma membra...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2260
更新日期:2011-06-19 00:00:00
abstract::Treatment of cultured vertebrate neurons with nitric oxide leads to growth-cone collapse, axon retraction and the reconfiguration of axonal microtubules. We show that the light chain of microtubule-associated protein (MAP) 1B is a substrate for S-nitrosylation in vivo, in cultured cells and in vitro. S-nitrosylation o...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1625
更新日期:2007-09-01 00:00:00
abstract::Organs and cells must adapt to shear stress induced by biological fluids, but how fluid flow contributes to the execution of specific cell programs is poorly understood. Here we show that shear stress favours mitochondrial biogenesis and metabolic reprogramming to ensure energy production and cellular adaptation in ki...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/s41556-020-0566-0
更新日期:2020-09-01 00:00:00
abstract::The enteric nervous system (ENS) predominantly originates from vagal neural crest (VNC) cells that emerge from the caudal hindbrain, invade the foregut and populate the gastrointestinal tract. However, the gene regulatory network (GRN) orchestrating the early specification of VNC remains unknown. Using an EdnrB enhanc...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/s41556-019-0428-9
更新日期:2019-12-01 00:00:00
abstract::Rad23 is a nucleotide-excision repair protein with a previously unknown biochemical function. We determined that yeast and human Rad23 inhibited multi-ubiquitin (Ub) chain formation and the degradation of proteolytic substrates. Significantly, Rad23 could be co-precipitated with a substrate that contained a short mult...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35023547
更新日期:2000-09-01 00:00:00
abstract::The endoplasmic reticulum (ER), a network of membranous sheets and pipes, supports functions encompassing biogenesis of secretory proteins and delivery of functional solutes throughout the cell1,2. Molecular mobility through the ER network enables these functionalities, but diffusion alone is not sufficient to explain...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/s41556-018-0192-2
更新日期:2018-10-01 00:00:00
abstract::The tumour stroma is an active participant during cancer progression. Stromal cells promote tumour progression and metastasis through multiple mechanisms including enhancing tumour invasiveness and angiogenesis, and suppressing immune surveillance. We report here that miR-126/miR-126(*), a microRNA pair derived from a...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2690
更新日期:2013-03-01 00:00:00
abstract::Obesity occurs when excess energy accumulates in white adipose tissue (WAT), whereas brown adipose tissue (BAT), specialized for energy expenditure through thermogenesis, potently counteracts obesity. Factors that induce brown adipocyte commitment and energy expenditure would be a promising defence against adiposity. ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2867
更新日期:2013-12-01 00:00:00
abstract::Proteins containing ubiquitin-binding domains (UBDs) interact with ubiquitinated targets and regulate diverse biological processes, including endocytosis, signal transduction, transcription and DNA repair. Many of the UBD-containing proteins are also themselves monoubiquitinated, but the functional role and the mechan...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1354
更新日期:2006-02-01 00:00:00
abstract::Epithelial-mesenchymal transition (EMT) is a crucial embryonic programme that is executed by various EMT transcription factors (EMT-TFs) and is aberrantly activated in cancer and other diseases. However, the causal role of EMT and EMT-TFs in different disease processes, especially cancer and metastasis, continues to b...
journal_title:Nature cell biology
pub_type: 杂志文章,评审
doi:10.1038/s41556-018-0196-y
更新日期:2019-01-01 00:00:00
abstract::Cadherin-containing cell-cell junctions respond to intercellular tension by increasing their size, strength and complexity. The mechanical regulation of cadherin adhesions is now shown to involve myosin-dependent tension in the cortical actomyosin cytoskeleton. This reduces actin turnover to decrease the mobility of c...
journal_title:Nature cell biology
pub_type: 评论,杂志文章
doi:10.1038/ncb2980
更新日期:2014-06-01 00:00:00