Abstract:
:Cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-activated chloride channel expressed in a wide variety of epithelial cells, mutations of which are responsible for the hallmark defective chloride secretion observed in cystic fibrosis (CF). Although CFTR has been implicated in bicarbonate secretion, its ability to directly mediate bicarbonate secretion of any physiological significance has not been shown. We demonstrate here that endometrial epithelial cells possess a CFTR-mediated bicarbonate transport mechanism. Co-culture of sperm with endometrial cells treated with antisense oligonucleotide against CFTR, or with bicarbonate secretion-defective CF epithelial cells, resulted in lower sperm capacitation and egg-fertilizing ability. These results are consistent with a critical role of CFTR in controlling uterine bicarbonate secretion and the fertilizing capacity of sperm, providing a link between defective CFTR and lower female fertility in CF.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Wang XF,Zhou CX,Shi QX,Yuan YY,Yu MK,Ajonuma LC,Ho LS,Lo PS,Tsang LL,Liu Y,Lam SY,Chan LN,Zhao WC,Chung YW,Chan HCdoi
10.1038/ncb1047keywords:
subject
Has Abstractpub_date
2003-10-01 00:00:00pages
902-6issue
10eissn
1465-7392issn
1476-4679pii
ncb1047journal_volume
5pub_type
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