Abstract：
:In vertebrates, centromeres lack defined sequences and are thought to be propagated by epigenetic mechanisms involving the incorporation of specialized nucleosomes containing the histone H3 variant centromere protein (CENP)-A. However, the precise mechanisms that target CENP-A to centromeres remain poorly understood. Here, we isolated a multi-subunit complex, which includes the established inner kinetochore components CENP-H and CENP-I, and nine other proteins, from both human and chicken cells. Our analysis of these proteins demonstrates that the CENP-H-I complex can be divided into three functional sub-complexes, each of which is required for faithful chromosome segregation. Interestingly, newly expressed CENP-A is not efficiently incorporated into centromeres in knockout mutants of a subclass of CENP-H-I complex proteins, indicating that the CENP-H-I complex may function, in part, as a marker directing CENP-A deposition to centromeres.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Okada M,Cheeseman IM,Hori T,Okawa K,McLeod IX,Yates JR 3rd,Desai A,Fukagawa Tdoi
10.1038/ncb1396subject
Has Abstractpub_date
2006-05-01 00:00:00pages
446-57issue
5eissn
1465-7392issn
1476-4679pii
ncb1396journal_volume
8pub_type
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