Abstract:
:Protein kinases have central functions in various cellular signal transduction pathways through their substrate phosphorylation. Here we show that a protein kinase, DYRK2, has unexpected role as a scaffold for an E3 ubiquitin ligase complex. DYRK2 associates with an E3 ligase complex containing EDD, DDB1 and VPRBP proteins (EDVP complex). Strikingly, DYRK2 serves as a scaffold for the EDVP complex, because small-interfering-RNA-mediated depletion of DYRK2 disrupts the formation of the EDD-DDB1-VPRBP complex. Although the kinase activity of DYRK2 is dispensable for its ability to mediate EDVP complex formation, it is required for the phosphorylation and subsequent degradation of its downstream substrate, katanin p60. Collectively, our results reveal a new type of E3-ubiquitin ligase complex in humans that depends on a protein kinase for complex formation as well as for the subsequent phosphorylation, ubiquitylation and degradation of their substrates.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Maddika S,Chen Jdoi
10.1038/ncb1848subject
Has Abstractpub_date
2009-04-01 00:00:00pages
409-19issue
4eissn
1465-7392issn
1476-4679pii
ncb1848journal_volume
11pub_type
杂志文章abstract::The spindle checkpoint ensures accurate chromosome segregation by delaying cell-cycle progression until all sister kinetochores capture microtubules from opposite poles and come under tension (for reviews, see refs 1, 2). Although the checkpoint is activated by either the lack of kinetochore-microtubule attachments or...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1341
更新日期:2006-01-01 00:00:00
abstract::Pluripotent stem cells (PSCs) may provide a potential source of haematopoietic stem/progenitor cells (HSPCs) for transplantation; however, unknown molecular barriers prevent the self-renewal of PSC-HSPCs. Using two-step differentiation, human embryonic stem cells (hESCs) differentiated in vitro into multipotent haemat...
journal_title:Nature cell biology
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pub_type: 杂志文章
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journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3142
更新日期:2015-04-01 00:00:00
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journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2775
更新日期:2013-07-01 00:00:00
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journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1557
更新日期:2007-04-01 00:00:00
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journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2425
更新日期:2012-01-29 00:00:00
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journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2151
更新日期:2011-02-01 00:00:00
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journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1966
更新日期:2009-10-01 00:00:00
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journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35074581
更新日期:2001-05-01 00:00:00
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journal_title:Nature cell biology
pub_type: 杂志文章,评审
doi:10.1038/s41556-019-0319-0
更新日期:2019-05-01 00:00:00
abstract::Actin networks drive many essential cellular processes, including cell migration, cytokinesis and tissue morphogenesis. However, how cells organize and regulate dynamic actin networks that consist of long, unbranched actin filaments is only poorly understood. This study in mouse oocytes reveals that cells can use vesi...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2802
更新日期:2013-08-01 00:00:00
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journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3390
更新日期:2016-08-01 00:00:00
abstract::Organs and cells must adapt to shear stress induced by biological fluids, but how fluid flow contributes to the execution of specific cell programs is poorly understood. Here we show that shear stress favours mitochondrial biogenesis and metabolic reprogramming to ensure energy production and cellular adaptation in ki...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/s41556-020-0566-0
更新日期:2020-09-01 00:00:00
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journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2633
更新日期:2013-01-01 00:00:00
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journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2294
更新日期:2011-07-17 00:00:00
abstract::Cell-cell adhesion mediated by specific cell-surface molecules is essential for multicellular development. Here we quantify de-adhesion forces at the resolution of individual cell-adhesion molecules, by controlling the interactions between single cells and combining single-molecule force spectroscopy with genetic mani...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35014000
更新日期:2000-06-01 00:00:00
abstract::The tumour-suppressor gene ATM, mutations in which cause the human genetic disease ataxia telangiectasia (A-T), encodes a key protein kinase that controls the cellular response to DNA double-strand breaks (DSBs). DNA DSBs caused by ionizing radiation or chemicals result in rapid ATM autophosphorylation, leading to che...
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pub_type: 杂志文章
doi:10.1038/ncb1651
更新日期:2007-11-01 00:00:00
abstract::Centromeres are specialized chromosomal domains that direct kinetochore assembly during mitosis. CENP-A (centromere protein A), a histone H3-variant present exclusively in centromeric nucleosomes, is thought to function as an epigenetic mark that specifies centromere identity. Here we identify the essential centromere...
journal_title:Nature cell biology
pub_type: 杂志文章
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journal_title:Nature cell biology
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更新日期:2016-03-01 00:00:00
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pub_type: 评论,新闻
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更新日期:2010-10-01 00:00:00
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journal_title:Nature cell biology
pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-12-01 00:00:00
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更新日期:2017-08-01 00:00:00
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