A role for CSLD3 during cell-wall synthesis in apical plasma membranes of tip-growing root-hair cells.

Abstract:

:In plants, cell shape is defined by the cell wall, and changes in cell shape and size are dictated by modification of existing cell walls and deposition of newly synthesized cell-wall material. In root hairs, expansion occurs by a process called tip growth, which is shared by root hairs, pollen tubes and fungal hyphae. We show that cellulose-like polysaccharides are present in root-hair tips, and de novo synthesis of these polysaccharides is required for tip growth. We also find that eYFP-CSLD3 proteins, but not CESA cellulose synthases, localize to a polarized plasma-membrane domain in root hairs. Using biochemical methods and genetic complementation of a csld3 mutant with a chimaeric CSLD3 protein containing a CESA6 catalytic domain, we provide evidence that CSLD3 represents a distinct (1→4)-β-glucan synthase activity in apical plasma membranes during tip growth in root-hair cells.

journal_name

Nat Cell Biol

journal_title

Nature cell biology

authors

Park S,Szumlanski AL,Gu F,Guo F,Nielsen E

doi

10.1038/ncb2294

subject

Has Abstract

pub_date

2011-07-17 00:00:00

pages

973-80

issue

8

eissn

1465-7392

issn

1476-4679

pii

ncb2294

journal_volume

13

pub_type

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