Abstract:
:In plants, cell shape is defined by the cell wall, and changes in cell shape and size are dictated by modification of existing cell walls and deposition of newly synthesized cell-wall material. In root hairs, expansion occurs by a process called tip growth, which is shared by root hairs, pollen tubes and fungal hyphae. We show that cellulose-like polysaccharides are present in root-hair tips, and de novo synthesis of these polysaccharides is required for tip growth. We also find that eYFP-CSLD3 proteins, but not CESA cellulose synthases, localize to a polarized plasma-membrane domain in root hairs. Using biochemical methods and genetic complementation of a csld3 mutant with a chimaeric CSLD3 protein containing a CESA6 catalytic domain, we provide evidence that CSLD3 represents a distinct (1→4)-β-glucan synthase activity in apical plasma membranes during tip growth in root-hair cells.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Park S,Szumlanski AL,Gu F,Guo F,Nielsen Edoi
10.1038/ncb2294subject
Has Abstractpub_date
2011-07-17 00:00:00pages
973-80issue
8eissn
1465-7392issn
1476-4679pii
ncb2294journal_volume
13pub_type
杂志文章abstract::During cell division, many animal cells transform into a spherical shape and assemble a contractile ring composed of actin filaments and myosin motors at the equator to separate the cell body into two. Although actomyosin regulatory proteins are spatio-temporally controlled during cytokinesis, the direct contribution ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3142
更新日期:2015-04-01 00:00:00
abstract::The heterogeneity of exosomal populations has hindered our understanding of their biogenesis, molecular composition, biodistribution and functions. By employing asymmetric flow field-flow fractionation (AF4), we identified two exosome subpopulations (large exosome vesicles, Exo-L, 90-120 nm; small exosome vesicles, Ex...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/s41556-018-0040-4
更新日期:2018-03-01 00:00:00
abstract::In the version of this article originally published, parts of Figure 5 were misaligned because of a shift during production. In a, one data point was outside of the graph border. In b, axes lines were not connected, and graph lines did not reach the data points. In c and d, the axes lines were not connected. In e and ...
journal_title:Nature cell biology
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41556-019-0326-1
更新日期:2019-06-01 00:00:00
abstract::Pyruvate kinase M2 (PKM2) is upregulated in multiple cancer types and contributes to the Warburg effect by unclear mechanisms. Here we demonstrate that EGFR-activated ERK2 binds directly to PKM2 Ile 429/Leu 431 through the ERK2 docking groove and phosphorylates PKM2 at Ser 37, but does not phosphorylate PKM1. Phosphor...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2629
更新日期:2012-12-01 00:00:00
abstract::Changes in phosphorylation of the carboxy-terminal domain (CTD) of RNA polymerase II (RNAP) are associated with transcription initiation, elongation and termination. Sites of active transcription are generally characterized by hyperphosphorylated RNAP, particularly at Ser 2 residues, whereas inactive or poised genes m...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1663
更新日期:2007-12-01 00:00:00
abstract::Small RNAs generated at DNA break sites are implicated in mammalian DNA repair. Now, a study shows that following the formation of DNA double-strand breaks, bidirectional transcription events adjacent to the break generate small RNAs that trigger the DNA damage response by local RNA:RNA interactions. ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3645
更新日期:2017-11-29 00:00:00
abstract::Formation of a bipolar spindle is essential for faithful chromosome segregation at mitosis. Because centrosomes define spindle poles, defects in centrosome number and structural organization can lead to a loss of bipolarity. In addition, microtubule-mediated pulling and pushing forces acting on centrosomes and chromos...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1474
更新日期:2006-10-01 00:00:00
abstract::TP53 (tumour protein 53) is one of the most frequently mutated genes in human cancer and its role during cellular transformation has been studied extensively. However, the homeostatic functions of p53 are less well understood. Here, we explore the molecular dependency network of TP53 through an RNAi-mediated synthetic...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2264
更新日期:2011-06-05 00:00:00
abstract::Endocytosis of cell surface receptors is an important regulatory event in signal transduction. The transforming growth factor beta (TGF-beta) superfamily signals to the Smad pathway through heteromeric Ser-Thr kinase receptors that are rapidly internalized and then downregulated in a ubiquitin-dependent manner. Here w...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb975
更新日期:2003-05-01 00:00:00
abstract::In vertebrates, centromeres lack defined sequences and are thought to be propagated by epigenetic mechanisms involving the incorporation of specialized nucleosomes containing the histone H3 variant centromere protein (CENP)-A. However, the precise mechanisms that target CENP-A to centromeres remain poorly understood. ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1396
更新日期:2006-05-01 00:00:00
abstract::The presence of actin in the nucleus has been well established, and several studies have implicated nuclear actin in transcriptional regulation. Neuronal Wiskott-Aldrich syndrome protein (N-WASP) is a member of the WASP family of proteins; these proteins function in the cytoplasm as key regulators of cortical actin fi...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1433
更新日期:2006-07-01 00:00:00
abstract::Cell-cell adhesion mediated by specific cell-surface molecules is essential for multicellular development. Here we quantify de-adhesion forces at the resolution of individual cell-adhesion molecules, by controlling the interactions between single cells and combining single-molecule force spectroscopy with genetic mani...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35014000
更新日期:2000-06-01 00:00:00
abstract::Phagocytic cells engulf their prey into vesicular structures called phagosomes, of which a certain proportion becomes demarcated for enhanced maturation by a process called LC3-associated phagocytosis (LAP). Light has now been shed on the molecular requirements of LAP, establishing a central role for the protein Rubic...
journal_title:Nature cell biology
pub_type: 评论,杂志文章
doi:10.1038/ncb3197
更新日期:2015-07-01 00:00:00
abstract::Formins have been implicated in the regulation of cytoskeletal structure in animals and fungi. Here we show that the formins Bni1 and Bnr1 of budding yeast stimulate the assembly of actin filaments that function as precursors to tropomyosin-stabilized cables that direct polarized cell growth. With loss of formin funct...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb718
更新日期:2002-01-01 00:00:00
abstract::The double fertilization process in angiosperms is based on the delivery of a pair of sperm cells by the pollen tube (the male gametophyte), which elongates towards an embryo sac (the female gametophyte) enclosing an egg and a central cell. Several studies have described the mechanisms of gametophyte interaction, and ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1345
更新日期:2006-01-01 00:00:00
abstract::Gastrulation leads to three germ layers--ectoderm, mesoderm and endoderm--that are separated by two basement membranes. In the mouse embryo, the emergent gut endoderm results from the widespread intercalation of cells of two distinct origins: pluripotent epiblast-derived definitive endoderm (DE) and extra-embryonic vi...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3070
更新日期:2014-12-01 00:00:00
abstract::SLC7A11-mediated cystine uptake is critical for maintaining redox balance and cell survival. Here we show that this comes at a significant cost for cancer cells with high levels of SLC7A11. Actively importing cystine is potentially toxic due to its low solubility, forcing cancer cells with high levels of SLC7A11 (SLC7...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/s41556-020-0496-x
更新日期:2020-04-01 00:00:00
abstract::Numerous cellular functions depend on actin filament (F-actin) disassembly. The best-characterized disassembly proteins, the ADF (actin-depolymerizing factor)/cofilins (encoded by the twinstar gene in Drosophila), sever filaments and recycle monomers to promote actin assembly. Cofilin is also a relatively weak actin d...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3390
更新日期:2016-08-01 00:00:00
abstract::Reprogrammed glucose metabolism as a result of increased glycolysis and glucose uptake is a hallmark of cancer. Here we show that cancer cells can suppress glucose uptake by non-tumour cells in the premetastatic niche, by secreting vesicles that carry high levels of the miR-122 microRNA. High miR-122 levels in the cir...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3094
更新日期:2015-02-01 00:00:00
abstract::Brown adipose tissue is a key metabolic organ that oxidizes fatty acids and glucose to generate heat. Through epigenomic analyses of multiple adipose depots, the transcription factor nuclear factor I-A (NFIA) is now shown to drive the brown fat genetic program through binding to lineage-specific cis-regulatory element...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3591
更新日期:2017-08-31 00:00:00
abstract::Current models for the export of messenger RNA share the notion that the highly abundant class of nuclear RNA-binding proteins--the hnRNP proteins--have a key role in exporting RNA. But recent studies have led to a new understanding of several non-hnRNP proteins, including SR proteins and the conserved mRNA export fac...
journal_title:Nature cell biology
pub_type: 杂志文章,评审
doi:10.1038/ncb0901-e201
更新日期:2001-09-01 00:00:00
abstract::Endothelial cells and leptin receptor+ (LepR+) stromal cells are critical sources of haematopoietic stem cell (HSC) niche factors, including stem cell factor (SCF), in bone marrow. After irradiation or chemotherapy, these cells are depleted while adipocytes become abundant. We discovered that bone marrow adipocytes sy...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3570
更新日期:2017-08-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature cell biology
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41556-020-0479-y
更新日期:2020-03-01 00:00:00
abstract::Research on genome stability and integrity now extends far beyond the biochemistry of DNA repair to encompass signal transduction pathways that span numerous aspects of cellular life. Derailed genomic integrity pathways can result in debilitating genetic disorders, premature ageing, predisposition to cancer and degene...
journal_title:Nature cell biology
pub_type:
doi:10.1038/ncb1004-923
更新日期:2004-10-01 00:00:00
abstract::To maintain genome function and stability, DNA sequence and its organization into chromatin must be duplicated during cell division. Understanding how entire chromosomes are copied remains a major challenge. Here, we use nascent chromatin capture (NCC) to profile chromatin proteome dynamics during replication in human...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2918
更新日期:2014-03-01 00:00:00
abstract::Collective cell migration is essential for both physiological and pathological processes. Adherens junctions (AJs) maintain the integrity of the migrating cell group and promote cell coordination while allowing cellular rearrangements. Here, we show that AJs undergo a continuous treadmilling along the lateral sides of...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2985
更新日期:2014-07-01 00:00:00
abstract::Activation of the ataxia telangiectasia mutated (ATM) kinase triggers diverse cellular responses to ionizing radiation (IR), including the initiation of cell cycle checkpoints. Histone H2AX, p53 binding-protein 1 (53BP1) and Chk2 are targets of ATM-mediated phosphorylation, but little is known about their roles in sig...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb884
更新日期:2002-12-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) are generated via the expression of the transcription factors OCT4 (also known as POU5F1), SOX2, KLF4 and cMYC (OSKM) in somatic cells. In contrast to murine naive iPSCs, conventional human iPSCs are in a more developmentally advanced state called primed pluripotency. Here, we re...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/s41556-018-0254-5
更新日期:2019-02-01 00:00:00
abstract::The function and regulation of repetitive DNA, the 'dark matter' of the genome, is still only rudimentarily understood. Now a study investigating DNA replication of repetitive centromeric chromosome segments has started to expose a fascinating replication program that involves suppression of ATR signalling, in particu...
journal_title:Nature cell biology
pub_type: 评论,杂志文章
doi:10.1038/ncb3367
更新日期:2016-05-27 00:00:00
abstract::Correct placement and orientation of the mitotic spindle is essential for segregation of localized components and positioning of daughter cells. Although these processes are important in many cells, few factors that regulate spindle placement are known. Previous work has shown that GPB-1, the Gbeta subunit of a hetero...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35060092
更新日期:2001-03-01 00:00:00