Abstract：
:The GTPase dynamin plays an essential part in endocytosis by catalysing the fission of nascent clathrin-coated vesicles from the plasma membrane. Using preformed phosphatidylinositol-4,5-bisphosphate-containing lipid nanotubes as a membrane template for dynamin self-assembly, we investigate the conformational changes that arise during GTP hydrolysis by dynamin. Electron microscopy reveals that, in the GTP-bound state, dynamin rings appear to be tightly packed together. After GTP hydrolysis, the spacing between rings increases nearly twofold. When bound to the nanotubes, dynamin's GTPase activity is cooperative and is increased by three orders of magnitude compared with the activity of unbound dynamin. An increase in the Kcat (but not the K(m) of GTP hydrolysis accounts for the pronounced cooperativity. These data indicate that a novel, lengthwise ('spring-like') conformational change in a dynamin helix may participate in vesicle fission.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Stowell MH,Marks B,Wigge P,McMahon HTdoi
10.1038/8997keywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
27-32issue
1eissn
1465-7392issn
1476-4679journal_volume
1pub_type
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