Abstract：
:Here we evaluate the idea that the Golgi is in dynamic equilibrium with the endoplasmic reticulum (ER). In cytoplasts that lack the Golgi apparatus, no regrowth of the Golgi is observed, nor is any transport from the ER to the cell surface detected. However, introduction of the smallest measurable amount of Golgi (equivalent to a few per cent per cell) yields significant exocytic transport. Our results indicate that the steady-state levels of Golgi in the ER are far smaller than the 30% that has been postulated, and that the Golgi may be an independent organelle and not simply an extension of the ER.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Pelletier L,Jokitalo E,Warren Gdoi
10.1038/35041089keywords:
subject
Has Abstractpub_date
2000-11-01 00:00:00pages
840-6issue
11eissn
1465-7392issn
1476-4679journal_volume
2pub_type
杂志文章abstract：:The pericentriolar material (PCM), the microtubule-organizing component of the centrosome, contains a multitude of proteins and is commonly described as an amorphous cloud surrounding the centrioles. However, the days of the PCM as an unstructured matrix are numbered. Using super-resolution microscopy, several reports...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2617
更新日期：2012-11-01 00:00:00
abstract：:The migration of border cells during Drosophila melanogaster oogenesis is a simple and powerful system for studying invasive cell migration in vivo. Border cells are somatic cells that delaminate from the follicular epithelium of an egg chamber and invade the germ line cluster. They migrate between the nurse cells to ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb848
更新日期：2002-09-01 00:00:00
abstract：:Spatial and temporal coordination of polo-like kinase 1 (Plk1) activity is necessary for mitosis and cytokinesis, and this is achieved through binding to phosphorylated docking proteins with distinct subcellular localizations. Although cyclin-dependent kinase 1 (Cdk1) creates these phosphorylated docking sites in meta...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1557
更新日期：2007-04-01 00:00:00
abstract：:Parallel quantification of large numbers of messenger RNA transcripts using microarray technology promises to provide detailed insight into cellular processes involved in the regulation of gene expression. This should allow new understanding of signalling networks that operate in the cell and of the molecular basis an...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/35087138
更新日期：2001-08-01 00:00:00
abstract：:Unrepaired DNA double-strand breaks (DSBs) cause genetic instability that leads to malignant transformation or cell death. Cells respond to DSBs with the ordered recruitment of signalling and repair proteins to the site of lesion. Protein modification with ubiquitin is crucial for the signalling cascade, but how ubiqu...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2367
更新日期：2011-10-23 00:00:00
abstract：:The heterogeneity of exosomal populations has hindered our understanding of their biogenesis, molecular composition, biodistribution and functions. By employing asymmetric flow field-flow fractionation (AF4), we identified two exosome subpopulations (large exosome vesicles, Exo-L, 90-120 nm; small exosome vesicles, Ex...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-018-0040-4
更新日期：2018-03-01 00:00:00
abstract：:Collective cell migration occurs in a range of contexts: cancer cells frequently invade in cohorts while retaining cell-cell junctions. Here we show that collective invasion by cancer cells depends on decreasing actomyosin contractility at sites of cell-cell contact. When actomyosin is not downregulated at cell-cell c...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2133
更新日期：2011-01-01 00:00:00
abstract：:During cell division, many animal cells transform into a spherical shape and assemble a contractile ring composed of actin filaments and myosin motors at the equator to separate the cell body into two. Although actomyosin regulatory proteins are spatio-temporally controlled during cytokinesis, the direct contribution ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3142
更新日期：2015-04-01 00:00:00
abstract：:Micronucleation of missegregated chromatin can lead to substantial chromosome rearrangements via chromothripsis. However, the molecular details of micronucleus-based chromothripsis are still unclear. Now, an elegant system that specifically induces missegregation of the Y chromosome provides insight into this process,...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3458
更新日期：2016-12-23 00:00:00
abstract：:Despite advances in the differentiation of insulin-producing cells from human embryonic stem cells, the generation of mature functional β cells in vitro has remained elusive. To accomplish this goal, we have developed cell culture conditions to closely mimic events occurring during pancreatic islet organogenesis and β...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-018-0271-4
更新日期：2019-02-01 00:00:00
abstract：: ...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/s41556-018-0246-5
更新日期：2018-12-01 00:00:00
abstract：:The molecular requirements and morphology of migrating cells can vary depending on matrix geometry; therefore, predicting the optimal migration strategy or the effect of experimental perturbation is difficult. We present a model of cell motility that encompasses actin-polymerization-based protrusions, actomyosin contr...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2775
更新日期：2013-07-01 00:00:00
abstract：:Actin networks drive many essential cellular processes, including cell migration, cytokinesis and tissue morphogenesis. However, how cells organize and regulate dynamic actin networks that consist of long, unbranched actin filaments is only poorly understood. This study in mouse oocytes reveals that cells can use vesi...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2802
更新日期：2013-08-01 00:00:00
abstract：:Clathrin-coated vesicles mediate endocytosis and transport between the trans-Golgi network (TGN) and endosomes in eukaryotic cells. Clathrin adaptors play central roles in coat assembly, interacting with clathrin, cargo and membranes. Two main types of clathrin adaptor act in TGN-endosome traffic: GGA proteins and the...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2427
更新日期：2012-02-19 00:00:00
abstract：:Phagocytic cells engulf their prey into vesicular structures called phagosomes, of which a certain proportion becomes demarcated for enhanced maturation by a process called LC3-associated phagocytosis (LAP). Light has now been shed on the molecular requirements of LAP, establishing a central role for the protein Rubic...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb3197
更新日期：2015-07-01 00:00:00
abstract：:Formins have been implicated in the regulation of cytoskeletal structure in animals and fungi. Here we show that the formins Bni1 and Bnr1 of budding yeast stimulate the assembly of actin filaments that function as precursors to tropomyosin-stabilized cables that direct polarized cell growth. With loss of formin funct...
journal_title：Nature cell biology
pub_type： 更正并重新发布的文章,杂志文章
doi：10.1038/ncb770
更新日期：2002-03-01 00:00:00
abstract：:The tumour vascular microenvironment supports tumorigenesis not only by supplying oxygen and diffusible nutrients but also by secreting soluble factors that promote tumorigenesis. Here we identify a feedforward mechanism in which endothelial cells (ECs), in response to tumour-derived mediators, release angiocrines dri...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2954
更新日期：2014-05-01 00:00:00
abstract：:Junior researchers are encouraged to gain experience abroad, and for senior scientists, sabbaticals remain popular. France has taken the next step in fostering international exchange, by supporting long-term collaborations with foreign laboratories and by creating research units abroad. ...
journal_title：Nature cell biology
pub_type： 社论
doi：10.1038/ncb0607-613
更新日期：2007-06-01 00:00:00
abstract：:Telomere attrition and other forms of telomere damage can activate the ATM kinase pathway. What generates the DNA damage signal at mammalian chromosome ends or at other double-strand breaks is not known. Telomere dysfunction is often accompanied by disappearance of the 3' telomeric overhang, raising the possibility th...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1275
更新日期：2005-07-01 00:00:00
abstract：:Cellular senescence is a stable proliferation arrest induced by triggers such as short telomeres, activated oncogenes and genotoxic stress. Two studies show that cellular senescence induced by genotoxic stress depends on chronic DNA-damage signalling from irreparable damage to telomeres. Hence, dysfunctional or damage...
journal_title：Nature cell biology
pub_type： 评论,新闻
doi：10.1038/ncb2476
更新日期：2012-04-02 00:00:00
abstract：:Although normally dormant, hair follicle stem cells (HFSCs) quickly become activated to divide during a new hair cycle. The quiescence of HFSCs is known to be regulated by a number of intrinsic and extrinsic mechanisms. Here we provide several lines of evidence to demonstrate that HFSCs utilize glycolytic metabolism a...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3575
更新日期：2017-09-01 00:00:00
abstract：:Guard cells, which form stomata in leaf epidermes, sense a multitude of environmental signals and integrate this information to regulate stomatal movements. Compared with the advanced understanding of light and water stress responses in guard cells, the molecular mechanisms that underlie stomatal CO(2) signalling have...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1387
更新日期：2006-04-01 00:00:00
abstract：:The guanosine tri-phosphatase Ran stimulates assembly of microtubule spindles. However, it is not known what aspects of the microtubule cytoskeleton are subject to regulation by Ran in mitosis. Here we show that Ran-GTP stimulates microtubule assembly by increasing the rescue frequency of microtubules three- to eightf...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35060000
更新日期：2001-03-01 00:00:00
abstract：:Gastrulation leads to three germ layers--ectoderm, mesoderm and endoderm--that are separated by two basement membranes. In the mouse embryo, the emergent gut endoderm results from the widespread intercalation of cells of two distinct origins: pluripotent epiblast-derived definitive endoderm (DE) and extra-embryonic vi...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3070
更新日期：2014-12-01 00:00:00
abstract：:The most dangerous insults to the genome's integrity are those that break both strands of the DNA. Double-strand breaks can be repaired by homologous recombination; in this conserved mechanism, a global genomic homology search finds sequences similar to those near the break, and uses them as a template for DNA synthes...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2745
更新日期：2013-06-01 00:00:00
abstract：:Cellular transformation and cancer progression is accompanied by changes in the metabolic landscape. Master co-regulators of metabolism orchestrate the modulation of multiple metabolic pathways through transcriptional programs, and hence constitute a probabilistically parsimonious mechanism for general metabolic rewir...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3357
更新日期：2016-06-01 00:00:00
abstract：:Cohesin confers both intrachromatid and interchromatid cohesion through formation of a tripartite ring within which DNA is thought to be entrapped. Here, I discuss what is known about the four stages of the cohesin ring cycle using the ring model as an intellectual framework. I postulate that cohesin loading onto chro...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb2349
更新日期：2011-10-03 00:00:00
abstract：:Endothelial cells and leptin receptor+ (LepR+) stromal cells are critical sources of haematopoietic stem cell (HSC) niche factors, including stem cell factor (SCF), in bone marrow. After irradiation or chemotherapy, these cells are depleted while adipocytes become abundant. We discovered that bone marrow adipocytes sy...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3570
更新日期：2017-08-01 00:00:00
abstract：:Tau is an abundant microtubule-associated protein in neurons. Tau aggregation into insoluble fibrils is a hallmark of Alzheimer's disease and other types of dementia1, yet the physiological state of tau molecules within cells remains unclear. Using single-molecule imaging, we directly observe that the microtubule latt...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-019-0375-5
更新日期：2019-09-01 00:00:00
abstract：:Pluripotent stem cells (PSCs) may provide a potential source of haematopoietic stem/progenitor cells (HSPCs) for transplantation; however, unknown molecular barriers prevent the self-renewal of PSC-HSPCs. Using two-step differentiation, human embryonic stem cells (hESCs) differentiated in vitro into multipotent haemat...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3354
更新日期：2016-06-01 00:00:00
