Abstract：
:YAP (Yes-associated protein) is a transcription co-activator in the Hippo tumour suppressor pathway and controls cell growth, tissue homeostasis and organ size. YAP is inhibited by the kinase Lats, which phosphorylates YAP to induce its cytoplasmic localization and proteasomal degradation. YAP induces gene expression by binding to the TEAD family transcription factors. Dysregulation of the Hippo-YAP pathway is frequently observed in human cancers. Here we show that cellular energy stress induces YAP phosphorylation, in part due to AMPK-dependent Lats activation, thereby inhibiting YAP activity. Moreover, AMPK directly phosphorylates YAP Ser 94, a residue essential for the interaction with TEAD, thus disrupting the YAP-TEAD interaction. AMPK-induced YAP inhibition can suppress oncogenic transformation of Lats-null cells with high YAP activity. Our study establishes a molecular mechanism and functional significance of AMPK in linking cellular energy status to the Hippo-YAP pathway.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Mo JS,Meng Z,Kim YC,Park HW,Hansen CG,Kim S,Lim DS,Guan KLdoi
10.1038/ncb3111subject
Has Abstractpub_date
2015-04-01 00:00:00pages
500-10issue
4eissn
1465-7392issn
1476-4679pii
ncb3111journal_volume
17pub_type
杂志文章abstract：:Pumilio proteins PUM1 and PUM2 are shown to regulate microRNA-dependent gene silencing by induction of a conformational switch in the 3' untranslated region of p27 mRNA. This conformational change is required for efficient microRNA-mediated repression of this cell-cycle regulator in rapidly proliferating cells. ...
journal_title：Nature cell biology
pub_type： 评论,新闻
doi：10.1038/ncb1010-928
更新日期：2010-10-01 00:00:00
abstract：:The polarity protein Scribble (SCRIB) regulates apical-basal polarity, directional migration and tumour suppression in Drosophila and mammals. Here we report that SCRIB is an important regulator of myeloid cell functions including bacterial infection and inflammation. SCRIB interacts directly with the NADPH oxidase (N...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3413
更新日期：2016-11-01 00:00:00
abstract：:The cell division axis determines the future positions of daughter cells and is therefore critical for cell fate. The positioning of the division axis has been mostly studied in systems such as embryos or yeasts, in which cell shape is well defined. In these cases, cell shape anisotropy and cell polarity affect spindl...
journal_title：Nature cell biology
pub_type： 信件
doi：10.1038/ncb1307
更新日期：2005-10-01 00:00:00
abstract：:Formation of a bipolar spindle is essential for faithful chromosome segregation at mitosis. Because centrosomes define spindle poles, defects in centrosome number and structural organization can lead to a loss of bipolarity. In addition, microtubule-mediated pulling and pushing forces acting on centrosomes and chromos...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1474
更新日期：2006-10-01 00:00:00
abstract：:Ciliates and flagellates temporarily swim backwards on collision by generating a mechanoreceptor potential. Although this potential has been shown to be associated with cilia in Paramecium, the molecular entity of the mechanoreceptor has remained unknown. Here we show that Chlamydomonas cells express TRP11, a member o...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2214
更新日期：2011-05-01 00:00:00
abstract：:mTOR (mammalian target of rapamycin) signalling and macroautophagy (henceforth autophagy) regulate numerous pathological and physiological processes, including cellular responses to altered nutrient levels. However, the mechanisms regulating mTOR and autophagy remain incompletely understood. Lysosomes are dynamic intr...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2204
更新日期：2011-04-01 00:00:00
abstract：:Cancer cells consume large quantities of glucose and primarily use glycolysis for ATP production, even in the presence of adequate oxygen. This metabolic signature (aerobic glycolysis or the Warburg effect) enables cancer cells to direct glucose to biosynthesis, supporting their rapid growth and proliferation. However...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2172
更新日期：2011-03-01 00:00:00
abstract：:Tau is an abundant microtubule-associated protein in neurons. Tau aggregation into insoluble fibrils is a hallmark of Alzheimer's disease and other types of dementia1, yet the physiological state of tau molecules within cells remains unclear. Using single-molecule imaging, we directly observe that the microtubule latt...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-019-0375-5
更新日期：2019-09-01 00:00:00
abstract：:Lysosomes respond to environmental cues by controlling their own biogenesis, but the underlying mechanisms are poorly understood. Here we describe a protein kinase C (PKC)-dependent and mTORC1-independent mechanism for regulating lysosome biogenesis, which provides insights into previously reported effects of PKC on l...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3407
更新日期：2016-10-01 00:00:00
abstract：:Starvation of animals or cells triggers autophagic degradation of cell contents to retrieve nutrients, but, paradoxically, mitochondria enlarge. This is now shown to result from inhibition of mitochondrial fission through PKA-mediated phosphorylation of the GTPase DRP1. Elongation of mitochondria optimizes ATP product...
journal_title：Nature cell biology
pub_type： 评论,新闻
doi：10.1038/ncb0511-521
更新日期：2011-05-01 00:00:00
abstract：:During the development of multicellular organisms, concerted actions of molecular signalling networks determine whether cells undergo proliferation, differentiation, death or ageing. Here we show that genetic inactivation of the stress signalling kinase, MKK7, a direct activator of JNKs in mice, results in embryonic l...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1098
更新日期：2004-03-01 00:00:00
abstract：:The migration of border cells during Drosophila melanogaster oogenesis is a simple and powerful system for studying invasive cell migration in vivo. Border cells are somatic cells that delaminate from the follicular epithelium of an egg chamber and invade the germ line cluster. They migrate between the nurse cells to ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb848
更新日期：2002-09-01 00:00:00
abstract：:Dyneins are large microtubule motor proteins required for mitosis, intracellular transport and ciliary and flagellar motility. They generate force through a power-stroke mechanism, which is an ATP-consuming cycle of pre- and post-power-stroke conformational changes that cause relative motion between different dynein d...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2939
更新日期：2014-05-01 00:00:00
abstract：:The substrates used to modify nucleic acids and chromatin are affected by nutrient availability and the activity of metabolic pathways. Thus, cellular metabolism constitutes a fundamental component of chromatin status and thereby of genome regulation. Here we describe the biochemical and genetic principles of how meta...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb3629
更新日期：2017-11-01 00:00:00
abstract：:Formins have been implicated in the regulation of cytoskeletal structure in animals and fungi. Here we show that the formins Bni1 and Bnr1 of budding yeast stimulate the assembly of actin filaments that function as precursors to tropomyosin-stabilized cables that direct polarized cell growth. With loss of formin funct...
journal_title：Nature cell biology
pub_type： 更正并重新发布的文章,杂志文章
doi：10.1038/ncb770
更新日期：2002-03-01 00:00:00
abstract：:Junior researchers are encouraged to gain experience abroad, and for senior scientists, sabbaticals remain popular. France has taken the next step in fostering international exchange, by supporting long-term collaborations with foreign laboratories and by creating research units abroad. ...
journal_title：Nature cell biology
pub_type： 社论
doi：10.1038/ncb0607-613
更新日期：2007-06-01 00:00:00
abstract：:During apoptosis, dying cells are swiftly removed by phagocytes. It is not fully understood how apoptotic cells are recognized by phagocytes. Here we report the identification and characterization of the Caenorhabditis elegans ttr-52 gene, which encodes a transthyretin-like protein and is required for efficient cell c...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2068
更新日期：2010-07-01 00:00:00
abstract：:Protein degradation in eukaryotes often requires the ubiquitin-selective chaperone p97 for substrate recruitment and ubiquitin-chain assembly. However, the physiological relevance of p97, and its role in developmental processes, remain unclear. Here, we discover an unanticipated function for CDC-48/p97 in myosin assem...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1554
更新日期：2007-04-01 00:00:00
abstract：:Research on genome stability and integrity now extends far beyond the biochemistry of DNA repair to encompass signal transduction pathways that span numerous aspects of cellular life. Derailed genomic integrity pathways can result in debilitating genetic disorders, premature ageing, predisposition to cancer and degene...
journal_title：Nature cell biology
pub_type：
doi：10.1038/ncb1004-923
更新日期：2004-10-01 00:00:00
abstract：:The motion of a chromosome during mitosis is mediated by a bundle of microtubules, termed a kinetochore fibre (K-fibre), which connects the kinetochore of the chromosome to a spindle pole. Once formed, mature K-fibres maintain a steady state length because the continuous addition of microtubule subunits onto microtubu...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1207
更新日期：2005-01-01 00:00:00
abstract：:Protein kinases have central functions in various cellular signal transduction pathways through their substrate phosphorylation. Here we show that a protein kinase, DYRK2, has unexpected role as a scaffold for an E3 ubiquitin ligase complex. DYRK2 associates with an E3 ligase complex containing EDD, DDB1 and VPRBP pro...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1848
更新日期：2009-04-01 00:00:00
abstract：:The retinoblastoma protein (pRB) negatively regulates the progression from G1 to S phase of the cell cycle, in part, by repressing E2F-dependent transcription. pRB also possesses E2F-independent functions that contribute to cell-cycle control--for example, during pRB-mediated cell-cycle arrest pRB associates with Skp2...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1532
更新日期：2007-02-01 00:00:00
abstract：:Mammalian telomeres consist of non-coding TTAGGG repeats that are bound by the multi-protein complex 'shelterin', thus protecting chromosome ends from DNA repair mechanisms and degradation. Mammalian telomeric chromatin is enriched for the constitutive heterochromatin marks H3K9me3, H4K20me3 and HP1 (refs 2, 3, 4, 5, ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1685
更新日期：2008-02-01 00:00:00
abstract：:Mitochondrial function degenerates with ageing and in ageing-related neuromuscular degenerative diseases, causing physiological decline of the cell. Factors that can delay the degenerative process are actively sought after. Here, we show that reduced cytosolic protein synthesis is a robust cellular strategy that suppr...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1769
更新日期：2008-09-01 00:00:00
abstract：:Chromosome missegregation into a micronucleus can cause complex and localized genomic rearrangements known as chromothripsis, but the underlying mechanisms remain unresolved. Here we developed an inducible Y centromere-selective inactivation strategy by exploiting a CENP-A/histone H3 chimaera to directly examine the f...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3450
更新日期：2017-01-01 00:00:00
abstract：:Cell-cell adhesion mediated by specific cell-surface molecules is essential for multicellular development. Here we quantify de-adhesion forces at the resolution of individual cell-adhesion molecules, by controlling the interactions between single cells and combining single-molecule force spectroscopy with genetic mani...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35014000
更新日期：2000-06-01 00:00:00
abstract：:Haematopoietic stem cells (HSCs) arise in the developing aorta during embryogenesis. The number of HSC clones born has been estimated through transplantation, but experimental approaches to assess the absolute number of forming HSCs in a native setting have remained challenging. Here, we applied single-cell and clonal...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3444
更新日期：2017-01-01 00:00:00
abstract：:Metastasis is an inefficient process, especially during colonization at a distant organ. This bottleneck underlies the importance of the metastatic niche for seeding and outgrowth of metastases. Here, we classify the common functions of different metastatic niches: anchorage, survival support, protection from external...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/s41556-018-0145-9
更新日期：2018-08-01 00:00:00
abstract：:Vital organs such as the pancreas and the brain lack the capacity for effective regeneration. To overcome this limitation, an emerging strategy consists of converting resident tissue-specific cells into the cell types that are lost due to disease by a process called in vivo lineage reprogramming. Here we discuss recen...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb3108
更新日期：2015-03-01 00:00:00
abstract：:Guard cells, which form stomata in leaf epidermes, sense a multitude of environmental signals and integrate this information to regulate stomatal movements. Compared with the advanced understanding of light and water stress responses in guard cells, the molecular mechanisms that underlie stomatal CO(2) signalling have...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1387
更新日期：2006-04-01 00:00:00
