Abstract:
:The cell division axis determines the future positions of daughter cells and is therefore critical for cell fate. The positioning of the division axis has been mostly studied in systems such as embryos or yeasts, in which cell shape is well defined. In these cases, cell shape anisotropy and cell polarity affect spindle orientation. It remains unclear whether cell geometry or cortical cues are determinants for spindle orientation in mammalian cultured cells. The cell environment is composed of an extracellular matrix (ECM), which is connected to the intracellular actin cytoskeleton via transmembrane proteins. We used micro-contact printing to control the spatial distribution of the ECM on the substrate and demonstrated that it has a role in determining the orientation of the division axis of HeLa cells. On the basis of our analysis of the average distributions of actin-binding proteins in interphase and mitosis, we propose that the ECM controls the location of actin dynamics at the membrane, and thus the segregation of cortical components in interphase. This segregation is further maintained on the cortex of mitotic cells and used for spindle orientation.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Théry M,Racine V,Pépin A,Piel M,Chen Y,Sibarita JB,Bornens Mdoi
10.1038/ncb1307keywords:
subject
Has Abstractpub_date
2005-10-01 00:00:00pages
947-53issue
10eissn
1465-7392issn
1476-4679pii
ncb1307journal_volume
7pub_type
信件abstract::The store-operated calcium channel (SOC) located in the plasma membrane (PM) mediates capacitative entry of extracellular calcium after depletion of intracellular calcium stores in the endoplasmic or sarcoplasmic reticulum (ER/SR). An intimate interaction between the PM and the ER/SR is essential for the operation of ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb788
更新日期:2002-05-01 00:00:00
abstract::Ciliates and flagellates temporarily swim backwards on collision by generating a mechanoreceptor potential. Although this potential has been shown to be associated with cilia in Paramecium, the molecular entity of the mechanoreceptor has remained unknown. Here we show that Chlamydomonas cells express TRP11, a member o...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2214
更新日期:2011-05-01 00:00:00
abstract::Science denial is not new but, in the digital age, evidence-based conclusions appear to be increasingly threatened by beliefs based on emotion and isolated personal experience. When confronting a post-truth world, scientists must defend the scientific method and increase public engagement. ...
journal_title:Nature cell biology
pub_type: 社论
doi:10.1038/s41556-018-0231-z
更新日期:2018-11-01 00:00:00
abstract::The spindle checkpoint ensures accurate chromosome segregation by delaying cell-cycle progression until all sister kinetochores capture microtubules from opposite poles and come under tension (for reviews, see refs 1, 2). Although the checkpoint is activated by either the lack of kinetochore-microtubule attachments or...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1341
更新日期:2006-01-01 00:00:00
abstract::It has long-been accepted that normal somatic cells have intrinsic mechanisms that limit their proliferative lifespan. Recent work has now challenged this view by demonstrating that extrinsic factors might be determining proliferative potential. ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb0202-e25
更新日期:2002-02-01 00:00:00
abstract::In mammals, motile cilia cover many organs, such as fallopian tubes, respiratory tracts and brain ventricles. The development and function of these organs critically depend on efficient directional fluid flow ensured by the alignment of ciliary beating. To identify the mechanisms involved in this process, we analysed ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2040
更新日期:2010-04-01 00:00:00
abstract::Robust mechanisms to control cell proliferation have evolved to maintain the integrity of organ architecture. Here, we investigated how two critical proliferative pathways, Myc and E2f, are integrated to control cell cycles in normal and Rb-deficient cells using a murine intestinal model. We show that Myc and E2f1-3 h...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3210
更新日期:2015-08-01 00:00:00
abstract::Accurate chromosome segregation during cell division in metazoans relies on proper chromosome congression at the equator. Chromosome congression is achieved after bi-orientation to both spindle poles shortly after nuclear envelope breakdown, or by the coordinated action of motor proteins that slide misaligned chromoso...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb3060
更新日期:2014-12-01 00:00:00
abstract::Actin and myosin are major components of the cell cytoskeleton, with structural and regulatory functions that affect many essential cellular processes. Although they were traditionally thought to function only in the cytoplasm, it is now well accepted that actin and multiple myosins are found in the nucleus. Increasin...
journal_title:Nature cell biology
pub_type: 杂志文章,评审
doi:10.1038/ncb2364
更新日期:2011-11-02 00:00:00
abstract::Mammalian telomeres consist of non-coding TTAGGG repeats that are bound by the multi-protein complex 'shelterin', thus protecting chromosome ends from DNA repair mechanisms and degradation. Mammalian telomeric chromatin is enriched for the constitutive heterochromatin marks H3K9me3, H4K20me3 and HP1 (refs 2, 3, 4, 5, ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1685
更新日期:2008-02-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature cell biology
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41556-020-0479-y
更新日期:2020-03-01 00:00:00
abstract::Phosphoinositide 3-kinase (PI(3)K) is a unique enzyme characterized by both lipid and protein kinase activities. Here, we demonstrate a requirement for the protein kinase activity of PI(3)K in agonist-dependent beta-adrenergic receptor (betaAR) internalization. Using PI(3)K mutants with either protein or lipid phospho...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1278
更新日期:2005-08-01 00:00:00
abstract::Activation of the ataxia telangiectasia mutated (ATM) kinase triggers diverse cellular responses to ionizing radiation (IR), including the initiation of cell cycle checkpoints. Histone H2AX, p53 binding-protein 1 (53BP1) and Chk2 are targets of ATM-mediated phosphorylation, but little is known about their roles in sig...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb884
更新日期:2002-12-01 00:00:00
abstract::To control cell proliferation, signal transduction needs to regulate the cell-cycle machinery. Recent findings show that Akt - a major kinase that coordinates diverse signalling pathways - phosphorylates Skp2, a subunit of the SCF-Skp2 ubiquitin ligase that targets key cell-cycle regulators. Akt1-dependent phosphoryla...
journal_title:Nature cell biology
pub_type: 评论,新闻
doi:10.1038/ncb0409-377
更新日期:2009-04-01 00:00:00
abstract::Genomic integrity is maintained by checkpoints that guard against undesired replication after DNA damage. Here, we show that CDT1, a licensing factor of the pre-replication complex (preRC), is rapidly proteolysed after UV- or gamma-irradiation. The preRC assembles on replication origins at the end of mitosis and durin...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1061
更新日期:2003-11-01 00:00:00
abstract::To maintain genome function and stability, DNA sequence and its organization into chromatin must be duplicated during cell division. Understanding how entire chromosomes are copied remains a major challenge. Here, we use nascent chromatin capture (NCC) to profile chromatin proteome dynamics during replication in human...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2918
更新日期:2014-03-01 00:00:00
abstract::The stability and membrane localization of the transforming growth factor-β (TGF-β) type I receptor (TβRI) determines the levels of TGF-β signalling. TβRI is targeted for ubiquitylation-mediated degradation by the SMAD7-SMURF2 complex. Here we performed a genome-wide gain-of-function screen and identified ubiquitin-sp...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2522
更新日期:2012-06-17 00:00:00
abstract::ES-cell-based cardiovascular repair requires an in-depth understanding of the molecular mechanisms underlying the differentiation of cardiovascular ES cells. A candidate cardiovascular-fate inducer is the bHLH transcription factor MesP1. As one of the earliest markers, it is expressed specifically in almost all cardio...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1696
更新日期:2008-03-01 00:00:00
abstract::The deposition of chemical modifications into RNA is a crucial regulator of temporal and spatial gene expression programs during development. Accordingly, altered RNA modification patterns are widely linked to developmental diseases. Recently, the dysregulation of RNA modification pathways also emerged as a contributo...
journal_title:Nature cell biology
pub_type: 杂志文章,评审
doi:10.1038/s41556-019-0319-0
更新日期:2019-05-01 00:00:00
abstract::In the version of this article originally published, parts of Figure 5 were misaligned because of a shift during production. In a, one data point was outside of the graph border. In b, axes lines were not connected, and graph lines did not reach the data points. In c and d, the axes lines were not connected. In e and ...
journal_title:Nature cell biology
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41556-019-0326-1
更新日期:2019-06-01 00:00:00
abstract::The adult organism is characterized by remarkable plasticity, which enables efficient regeneration and restoration of homeostasis after damage. When aberrantly activated, this plasticity contributes to tumour initiation and progression. Here we review recent advances in this field with a focus on cell fate changes and...
journal_title:Nature cell biology
pub_type: 杂志文章,评审
doi:10.1038/ncb3611
更新日期:2017-10-01 00:00:00
abstract::Epithelial-mesenchymal transition (EMT) is essential for organogenesis and is triggered during carcinoma progression to an invasive state. Transforming growth factor-beta (TGF-beta) cooperates with signalling pathways, such as Ras and Wnt, to induce EMT, but the molecular mechanisms are not clear. Here, we report that...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1905
更新日期:2009-08-01 00:00:00
abstract::The promyelocytic leukaemia (PML) tumour-suppressor protein potentiates p53 function by regulating post-translational modifications, such as CBP-dependent acetylation and Chk2-dependent phosphorylation, in the PML-Nuclear Body (NB). PML was recently shown to interact with the p53 ubiquitin-ligase Mdm2 (refs 4-6); howe...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1147
更新日期:2004-07-01 00:00:00
abstract::To generate the various tissues and organs that build up the adult body, plants and animals require organized formative cell divisions and correct cell specification. In plants, these processes are controlled mainly by phytohormones and transcriptional networks. Recently, ligand-receptor-like kinase signalling pathway...
journal_title:Nature cell biology
pub_type: 杂志文章,评审
doi:10.1038/ncb1009-1166
更新日期:2009-10-01 00:00:00
abstract::The guanosine tri-phosphatase Ran stimulates assembly of microtubule spindles. However, it is not known what aspects of the microtubule cytoskeleton are subject to regulation by Ran in mitosis. Here we show that Ran-GTP stimulates microtubule assembly by increasing the rescue frequency of microtubules three- to eightf...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35060000
更新日期:2001-03-01 00:00:00
abstract::To learn more about cancer-associated fibroblasts (CAFs), we have isolated fibroblasts from different stages of breast cancer progression and analysed their function and gene expression. These analyses reveal that activation of the YAP transcription factor is a signature feature of CAFs. YAP function is required for C...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb2756
更新日期:2013-06-01 00:00:00
abstract::In vertebrates, centromeres lack defined sequences and are thought to be propagated by epigenetic mechanisms involving the incorporation of specialized nucleosomes containing the histone H3 variant centromere protein (CENP)-A. However, the precise mechanisms that target CENP-A to centromeres remain poorly understood. ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1396
更新日期:2006-05-01 00:00:00
abstract::Cell division is fundamental for all organisms. Here we report a genome-scale RNA-mediated interference screen in HeLa cells designed to identify human genes that are important for cell division. We have used a library of endoribonuclease-prepared short interfering RNAs for gene silencing and have used DNA content ana...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1659
更新日期:2007-12-01 00:00:00
abstract::The mitochondrial inner membrane is the central energy-converting membrane of eukaryotic cells. The electrochemical proton gradient generated by the respiratory chain drives the ATP synthase. To maintain this proton-motive force, the inner membrane forms a tight barrier and strictly controls the translocation of ions....
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1635
更新日期:2007-10-01 00:00:00
abstract::Dynamic regulation of adhesion complexes is required for cell migration and has therefore emerged as a key issue in the study of cell motility. Recent progress has been made in defining some of the molecular mechanisms by which adhesion disassembly is regulated, including the contributions of adhesion adaptor proteins...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/ncb1175
更新日期:2004-10-01 00:00:00