Abstract：
:Lingual keratinized epithelial cells, which constitute the filiform papillae of the tongue, have one of the most rapid tissue turnover rates in the mammalian body and are thought to be the source of squamous cell carcinoma of the tongue. However, the mechanism of tissue maintenance and regeneration is largely unknown for these cells. Here, we show that stem cells positive for Bmi1, keratin 14 and keratin 5 are present in the base but not at the very bottom of the interpapillary pit (observed most frequently in the second or third layer (position +2 or +3) from the basal cells). Using a multicolour lineage tracing method, we demonstrated that one stem cell per interpapillary pit survives long-term. The cells were shown to be unipotent stem cells for keratinized epithelial cells but not for taste bud cells, and were found to usually be in a slow-growing or resting state; however, on irradiation-induced injury, the cells rapidly entered the cell cycle and regenerated tongue epithelium. The elimination of Bmi1-positive stem cells significantly suppressed the regeneration. Taken together, these results suggest that the stem cells identified in this study are important for tissue maintenance and regeneration of the lingual epithelium.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Tanaka T,Komai Y,Tokuyama Y,Yanai H,Ohe S,Okazaki K,Ueno Hdoi
10.1038/ncb2719subject
Has Abstractpub_date
2013-05-01 00:00:00pages
511-8issue
5eissn
1465-7392issn
1476-4679pii
ncb2719journal_volume
15pub_type
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