Abstract：
:Here we investigate the role of the control of apoptosis in normal cell division. We show that interference with the expression or function of the apoptosis inhibitor survivin causes caspase-dependent cell death in the G2/M phase of the cell cycle, and a cell-division defect characterized by centrosome dysregulation, multipolar mitotic spindles and multinucleated, polyploid cells. Use of a dominant-negative survivin mutant or antisense survivin complementary DNA disrupts a supramolecular assembly of survivin, caspase-3 and the cyclin-dependent-kinase inhibitor p21Waf1/Cip1 within centrosomes, and results in caspase-dependent cleavage of p21. Polyploidy induced by survivin antagonists is accentuated in p21-deficient cells, and corrected by exogenous expression of p21. These findings show that control of apoptosis and preservation of p21 integrity within centrosomes by survivin are required for normal mitotic progression.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Li F,Ackermann EJ,Bennett CF,Rothermel AL,Plescia J,Tognin S,Villa A,Marchisio PC,Altieri DCdoi
10.1038/70242keywords:
subject
Has Abstractpub_date
1999-12-01 00:00:00pages
461-6issue
8eissn
1465-7392issn
1476-4679journal_volume
1pub_type
杂志文章abstract：:The promyelocytic leukaemia (PML) tumour-suppressor protein potentiates p53 function by regulating post-translational modifications, such as CBP-dependent acetylation and Chk2-dependent phosphorylation, in the PML-Nuclear Body (NB). PML was recently shown to interact with the p53 ubiquitin-ligase Mdm2 (refs 4-6); howe...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1147
更新日期：2004-07-01 00:00:00
abstract：:Wnt/β-catenin signalling is an important regulator of liver development, zonation and regeneration. The cell surface complex RSPO-LGR4/5-ZNF3/RNF43 is now shown to direct Wnt/β-catenin signalling in orchestrating the division of the liver into functionally distinct metabolic zones, providing insights into the mechanis...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb3349
更新日期：2016-04-27 00:00:00
abstract：:When co-translationally inserted into endoplasmic reticulum (ER) membranes, newly synthesized proteins encounter the lumenal environment of the ER, which contains chaperone proteins that facilitate the folding reactions necessary for protein oligomerization, maturation and export from the ER. Here we show, using a tem...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35010558
更新日期：2000-05-01 00:00:00
abstract：:During the development of multicellular organisms, concerted actions of molecular signalling networks determine whether cells undergo proliferation, differentiation, death or ageing. Here we show that genetic inactivation of the stress signalling kinase, MKK7, a direct activator of JNKs in mice, results in embryonic l...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1098
更新日期：2004-03-01 00:00:00
abstract：:Cellular transformation and cancer progression is accompanied by changes in the metabolic landscape. Master co-regulators of metabolism orchestrate the modulation of multiple metabolic pathways through transcriptional programs, and hence constitute a probabilistically parsimonious mechanism for general metabolic rewir...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3357
更新日期：2016-06-01 00:00:00
abstract：:Changes in phosphorylation of the carboxy-terminal domain (CTD) of RNA polymerase II (RNAP) are associated with transcription initiation, elongation and termination. Sites of active transcription are generally characterized by hyperphosphorylated RNAP, particularly at Ser 2 residues, whereas inactive or poised genes m...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1663
更新日期：2007-12-01 00:00:00
abstract：:The enteric nervous system (ENS) predominantly originates from vagal neural crest (VNC) cells that emerge from the caudal hindbrain, invade the foregut and populate the gastrointestinal tract. However, the gene regulatory network (GRN) orchestrating the early specification of VNC remains unknown. Using an EdnrB enhanc...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-019-0428-9
更新日期：2019-12-01 00:00:00
abstract：:We have identified Rab10 as an ER-specific Rab GTPase that regulates ER structure and dynamics. We show that Rab10 localizes to the ER and to dynamic ER-associated structures that track along microtubules and mark the position of new ER tubule growth. Rab10 depletion or expression of a Rab10 GDP-locked mutant alters E...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2647
更新日期：2013-02-01 00:00:00
abstract：:As obligatory intracellular parasites, viruses rely on host-cell functions for most aspects of their replication cycle. This is born out during entry, when most viruses that infect vertebrate and insect cells exploit the endocytic activities of the host cell to move into the cytoplasm. Viruses belonging to vaccinia, a...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb0509-510
更新日期：2009-05-01 00:00:00
abstract：:Guard cells, which form stomata in leaf epidermes, sense a multitude of environmental signals and integrate this information to regulate stomatal movements. Compared with the advanced understanding of light and water stress responses in guard cells, the molecular mechanisms that underlie stomatal CO(2) signalling have...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1387
更新日期：2006-04-01 00:00:00
abstract：:Coordination of microtubules and the actin cytoskeleton is important in several types of cell movement. mDia1 is a member of the formin-homology family of proteins and an effector of the small GTPase Rho. It contains the Rho-binding domain in its amino terminus and two distinct regions of formin homology, FH1 in the m...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35050598
更新日期：2001-01-01 00:00:00
abstract：:Lamellipodia/ruffles and filopodia are protruding organelles containing short and highly branched or long and unbranched actin filaments, respectively. The microscopic morphology, dynamic development and protein signature of both lamellipodia/ruffles and filopodia have been investigated; however, little is known about...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1745
更新日期：2008-07-01 00:00:00
abstract：:The deposition of chemical modifications into RNA is a crucial regulator of temporal and spatial gene expression programs during development. Accordingly, altered RNA modification patterns are widely linked to developmental diseases. Recently, the dysregulation of RNA modification pathways also emerged as a contributo...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/s41556-019-0319-0
更新日期：2019-05-01 00:00:00
abstract：:Rad23 is a nucleotide-excision repair protein with a previously unknown biochemical function. We determined that yeast and human Rad23 inhibited multi-ubiquitin (Ub) chain formation and the degradation of proteolytic substrates. Significantly, Rad23 could be co-precipitated with a substrate that contained a short mult...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35023547
更新日期：2000-09-01 00:00:00
abstract：:Primordial germ cell (PGC) development in the human fetus remains relatively uncharted. A new study suggests that epigenetic reprogramming and sex differentiation in human PGCs occur asynchronously over an extended time period. This finding raises questions and implications for in vitro PGC differentiation. ...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb2665
更新日期：2013-01-01 00:00:00
abstract：:Mammalian telomeres consist of non-coding TTAGGG repeats that are bound by the multi-protein complex 'shelterin', thus protecting chromosome ends from DNA repair mechanisms and degradation. Mammalian telomeric chromatin is enriched for the constitutive heterochromatin marks H3K9me3, H4K20me3 and HP1 (refs 2, 3, 4, 5, ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1685
更新日期：2008-02-01 00:00:00
abstract：:The cytokine tumour necrosis factor (TNF) and the toll-like receptors (TLRs) coordinate immune responses by activating inflammatory transcriptional programs, but these signals can also trigger cell death. Recent studies identify the MAP kinase substrate MK2 as a key player in determining whether cells live or die in r...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3619
更新日期：2017-09-29 00:00:00
abstract：:In the version of this Article originally published, the author had misnumbered the reference citations in the Methods, using numbers 1-14 instead of 46-59. These errors have now been corrected in all online versions of the Article. ...
journal_title：Nature cell biology
pub_type： 杂志文章,已发布勘误
doi：10.1038/s41556-018-0053-z
更新日期：2018-08-01 00:00:00
abstract：:Lamina-associated polypeptide (LAP) 2alpha is a chromatin-associated protein that binds A-type lamins. Mutations in both LAP2alpha and A-type lamins are linked to human diseases called laminopathies, but the molecular mechanisms are poorly understood. The A-type lamin-LAP2alpha complex interacts with and regulates ret...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1793
更新日期：2008-11-01 00:00:00
abstract：:Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) involves a marked reorganization of chromatin. To identify post-translational histone modifications that change in global abundance during this process, we have applied a quantitative mass-spectrometry-based approach. We found that iPSCs, compa...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2768
更新日期：2013-07-01 00:00:00
abstract：:Parallel quantification of large numbers of messenger RNA transcripts using microarray technology promises to provide detailed insight into cellular processes involved in the regulation of gene expression. This should allow new understanding of signalling networks that operate in the cell and of the molecular basis an...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/35087138
更新日期：2001-08-01 00:00:00
abstract：:The double fertilization process in angiosperms is based on the delivery of a pair of sperm cells by the pollen tube (the male gametophyte), which elongates towards an embryo sac (the female gametophyte) enclosing an egg and a central cell. Several studies have described the mechanisms of gametophyte interaction, and ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1345
更新日期：2006-01-01 00:00:00
abstract：:Mitochondrial DNA (mtDNA) mutations cause inherited diseases and are implicated in the pathogenesis of common late-onset disorders, but how they arise is not clear1,2. Here we show that mtDNA mutations are present in primordial germ cells (PGCs) within healthy female human embryos. Isolated PGCs have a profound reduct...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-017-0017-8
更新日期：2018-02-01 00:00:00
abstract：:Tissue stem cells are the cell of origin for many malignancies. Metabolites regulate the balance between self-renewal and differentiation, but whether endogenous metabolic pathways or nutrient availability predispose stem cells towards transformation remains unknown. Here, we address this question in epidermal stem ce...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-020-0525-9
更新日期：2020-07-01 00:00:00
abstract：:Mitochondria drive apoptosis by releasing pro-apoptotic proteins that promote caspase activation in the cytosol. The rhomboid protease PARL, an intramembrane cleaving peptidase in the inner membrane, regulates mitophagy and plays an ill-defined role in apoptosis. Here, we employed PARL-based proteomics to define its s...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3488
更新日期：2017-04-01 00:00:00
abstract：:Telomere attrition and other forms of telomere damage can activate the ATM kinase pathway. What generates the DNA damage signal at mammalian chromosome ends or at other double-strand breaks is not known. Telomere dysfunction is often accompanied by disappearance of the 3' telomeric overhang, raising the possibility th...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1275
更新日期：2005-07-01 00:00:00
abstract：:Mouse embryos with a loss-of-function mutation in the gene encoding the receptor tyrosine kinase ErbB4 exhibit misprojections of cranial sensory ganglion afferent axons. Here we analyse ErbB4-deficient mice, and find that morphological differences between wild-type and mutant cranial ganglia correlate with aberrant mi...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35000058
更新日期：2000-02-01 00:00:00
abstract：:The genome is organized via CTCF-cohesin-binding sites, which partition chromosomes into 1-5 megabase (Mb) topologically associated domains (TADs), and further into smaller sub-domains (sub-TADs). Here we examined in vivo an ∼80 kb sub-TAD, containing the mouse α-globin gene cluster, lying within a ∼1 Mb TAD. We find ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3573
更新日期：2017-08-01 00:00:00
abstract：:Although normally dormant, hair follicle stem cells (HFSCs) quickly become activated to divide during a new hair cycle. The quiescence of HFSCs is known to be regulated by a number of intrinsic and extrinsic mechanisms. Here we provide several lines of evidence to demonstrate that HFSCs utilize glycolytic metabolism a...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3575
更新日期：2017-09-01 00:00:00
abstract：:Only a few RFamide peptides have been identified in mammals, although they have been abundantly found in invertebrates. Here we report the identification of a human gene that encodes at least three RFamide-related peptides, hRFRP-1-3. Cells transfected with a seven-transmembrane-domain receptor, OT7T022, specifically ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35036326
更新日期：2000-10-01 00:00:00
