Abstract：
:In vertebrates, the microtubule binding protein TPX2 is required for meiotic and mitotic spindle assembly. TPX2 is also known to bind to and activate Aurora A kinase and target it to the spindle. However, the relationship between the TPX2-Aurora A interaction and the role of TPX2 in spindle assembly is unclear. Here, we identify TPXL-1, a C. elegans protein that is the first characterized invertebrate ortholog of TPX2. We demonstrate that an essential role of TPXL-1 during mitosis is to activate and target Aurora A to microtubules. Our data suggest that this targeting stabilizes microtubules connecting kinetochores to the spindle poles. Thus, activation and targeting of Aurora A appears to be an ancient and conserved function of TPX2 that plays a central role in mitotic spindle assembly.
journal_name
Dev Celljournal_title
Developmental cellauthors
Ozlü N,Srayko M,Kinoshita K,Habermann B,O'toole ET,Müller-Reichert T,Schmalz N,Desai A,Hyman AAdoi
10.1016/j.devcel.2005.07.002keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
237-48issue
2eissn
1534-5807issn
1878-1551pii
S1534-5807(05)00253-4journal_volume
9pub_type
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