Abstract：
:The purpose of the present study was to develop a rapid, reproducible method of nonviral gene transfer to the intact vasculature. Male Sprague-Dawley rats were anesthetized, a midline abdominal incision was made and segmental branches of the superior mesenteric artery were dissected free of surrounding mesentery. A specially designed electroporation probe was placed around the neurovascular bundle and the electroporation chamber filled with a solution containing the firefly luciferase expressing plasmid (pCMV-Lux-DTS) or the green fluorescent protein expressing plasmid (pEGFP-N1). Vessels were electroporated with eight 10-ms pulses of 200 V/cm. Sixty seconds after electroporation, the DNA solution was removed, the intestine returned to the abdomen and the abdominal wall closed with suture and metal wound clips. Six hours to 5 days later, rats were sacrificed and electroporated vessels were recovered. Luciferase activity of the blood vessels was monitored. Gene expression was detected as early as 6 h postelectroporation, peaked at 1-3 days with levels up to 1 ng of reporter gene product per vessel segment and returned towards baseline by day 5. Histological analysis of blood vessel segments revealed green fluorescent protein-positive cells throughout the thickness of the vessel wall (endothelial cells to adventitia). Responses of electroporated vessels to vasoconstricting stimuli were indistinguishable from those of control vessels at either 2 or 40 days posttreatment. The results of this study provide evidence that electroporation is an effective means for introducing naked DNA into the blood vessel wall and form the basis for future studies on targeted gene therapy to the intact vasculature.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Martin JB,Young JL,Benoit JN,Dean DAdoi
10.1159/000025753keywords:
subject
Has Abstractpub_date
2000-09-01 00:00:00pages
372-80issue
5eissn
1018-1172issn
1423-0135pii
25753journal_volume
37pub_type
杂志文章abstract：:Regulation of [Ca2+]i and modulation of the [Ca2+]i sensitivity of myosin phosphorylation in smooth muscles may involve phosphorylation of one or more proteins on tyrosine residues. We tested this hypothesis by measuring tyrosine phosphorylation of proteins extracted from swine carotid artery and separated by SDS gel ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159196
更新日期：1997-01-01 00:00:00
abstract：:Coronary bypass vessels, saphenous vein (SV) and internal thoracic artery (ITA), differ in susceptibility to atherosclerosis and medium- to long-term patency. Whereas most ITA remain patent (90% at 10 years), 20% of SV grafts fail in the first year and approximately 45% fail within 10 years. Reasons for these differen...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159245
更新日期：1997-09-01 00:00:00
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journal_title：Journal of vascular research
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doi：10.1159/000501615
更新日期：2019-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000158972
更新日期：1993-01-01 00:00:00
abstract：:The vascular endothelium is the source of a number of vasodilator and vasoconstrictor autacoids and is thus a key regulator of vascular homeostasis. We studied the effects of altering the balance between protein tyrosine kinase and phosphatase activity on Ca2+ signalling and phosphotyrosine levels in cultured human en...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159150
更新日期：1996-05-01 00:00:00
abstract：BACKGROUND:Infusion of exogenous vascular endothelial growth factor (VEGF) into adult brain at doses above 60 ng/day induces dramatic angiogenesis accompanied by vascular leak and inflammation. Blood vessels formed by this treatment are dilated and tortuous, exhibiting a pathological morphology. Pathological VEGF-induc...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000101450
更新日期：2007-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000376548
更新日期：2015-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025637
更新日期：1999-03-01 00:00:00
abstract：BACKGROUND:Elevated plasma homocysteine (Hcy) is a risk factor for coronary disease. The objective of this study was to investigate whether Hcy either alone or in high glucose conditions induces endothelin-1 (ET-1) synthesis via the production of reactive oxygen species (ROS). METHODS:Bovine aortic endothelial cells w...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000090947
更新日期：2006-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000479873
更新日期：2017-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000452410
更新日期：2016-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000063687
更新日期：2002-05-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000158963
更新日期：1992-11-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159064
更新日期：1994-11-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章,随机对照试验
doi：10.1159/000510294
更新日期：2020-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025687
更新日期：1999-11-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000486571
更新日期：2018-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159097
更新日期：1995-07-01 00:00:00
abstract：:The aim of this study was to use periarterial manipulation to produce an atheroma-like neo-intima in rabbits and study resting blood flow and vascular responsiveness in vivo. One common carotid artery was enclosed in a silastic collar to induce a neo-intima similar to that of human early atherosclerosis, and carotid b...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159043
更新日期：1994-07-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000101777
更新日期：2007-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000051081
更新日期：2001-09-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000464287
更新日期：2017-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000081247
更新日期：2004-09-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000073902
更新日期：2003-09-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000074550
更新日期：2003-09-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000025622
更新日期：1999-01-01 00:00:00
abstract：:Topical application of elastase to induce arterial aneurysm formation is an emerging murine model of vascular disease. In the context of aortic abdominal aneurysm (AAA), angiotensin II infusion and porcine pancreatic elastase perfusion models are commonly used today. This study, therefore, compares matrix remodeling, ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000447263
更新日期：2016-01-01 00:00:00
abstract：:The influence of vascular endothelium on ouabain-induced contractions and sodium pump activity in aortic segments of Wistar-Kyoto (WKY) and spontaneously hypertensive rat (SHR) was analyzed. De-endothelialization increased and reduced ouabain-induced contractions in WKY and SHR segments, respectively. The effects of d...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159145
更新日期：1996-03-01 00:00:00
abstract：BACKGROUND:A new method was evaluated where local changes in oxygen tension were induced in a tissue while being studied under a microscope in vivo. We tested whether hypoxic vasodilation and hyperoxic vasoconstriction in arterioles in striated muscle are being propagated upstream, and whether the endothelium and smoot...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000318777
更新日期：2011-01-01 00:00:00
abstract：BACKGROUND:The aim of the present study was to evaluate different signaling pathways by which exercise training would interfere in endothelial function in obesity. Therefore, we examined adipocytokine levels and their receptors in the corpus cavernosum and femoral artery from trained rats on a high-fat diet. METHODS:F...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000457800
更新日期：2017-01-01 00:00:00
