Abstract：
:Atherosclerosis underlies late occlusion of human saphenous vein (HSV) coronary artery bypass grafts. Monocyte infiltration is implicated, but its mechanisms are unclear given that HSV normally expresses the ICAM-1 but not the VCAM-1 adhesion molecule. To define the mechanisms underlying monocyte adhesion, samples of HSV taken from coronary artery bypass graft patients were co-cultured with human monocytes and adherent monocytes were quantified by immunocytochemistry for CD68 on transverse sections. Pre-treatment of veins with anti-ICAM-1 antibodies reduced monocyte adhesion (monocytes/mm of section) from 7.2 +/- 1.5 to 3.1 +/- 0.7 (p < 0.05, n = 6), but the effect of anti-VCAM-1 was not significant (4.1 +/- 0.7). Paradoxically, pre-treatment of monocytes with either anti-beta(2)-integrins, the counter-receptor of ICAM-1, or anti-alpha(4) integrins, the counter-receptor of VCAM-1, significantly reduced adhesion (1.8 +/- 0.6 and 2.4 +/- 0.7, respectively, p < 0.05). These results were clarified by immunocytochemistry, which confirmed that VCAM-1 expression was absent in harvested vein but was induced in the endothelium during co-culture. Consistent with this, when anti-ICAM-1 or anti-VCAM-1 was present throughout co-culture, either of them reduced adhesion (from 4.2 +/- 0.9 to 2.3 +/- 0.5 and 2.2 +/- 0.4, respectively, p < 0.02, n = 8) and there was no further effect of adding both (2.0 +/- 0.5). These results demonstrate that both ICAM-1/beta(2) and VCAM-1/alpha(4) integrin interactions mediate monocyte adhesion to HSV, possibly as part of a common pathway. These experiments imply that either integrin might be targeted to reduce monocyte infiltration into HSV grafts.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Crook MF,Southgate KM,Newby ACdoi
10.1159/000063687keywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
221-9issue
3eissn
1018-1172issn
1423-0135pii
63687journal_volume
39pub_type
杂志文章abstract：BACKGROUND/AIMS:The endothelium has been recognized as a key component in the regulation of blood vessels. We designed a simple procedure to separate endothelial and smooth muscle RNA from rat aorta and mesenteric artery and used this method to establish the distribution of Na(+)/K(+)-ATPase alpha-subunit isoforms (NaK...
journal_title：Journal of vascular research
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journal_title：Journal of vascular research
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更新日期：1994-07-01 00:00:00
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更新日期：2009-01-01 00:00:00
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更新日期：1998-01-01 00:00:00
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更新日期：1999-05-01 00:00:00
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pub_type： 杂志文章,随机对照试验
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journal_title：Journal of vascular research
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doi：10.1159/000054089
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000124283
更新日期：2008-01-01 00:00:00
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doi：10.1159/000381097
更新日期：2015-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章,评审
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更新日期：1996-09-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159222
更新日期：1997-05-01 00:00:00
abstract：AIMS:We investigated the role of superoxide O(2)(-) during the initiation of vascular endothelial growth factor (VEGF)- and placental growth factor (PlGF)-mediated signal transduction in bone marrow-derived endothelial cells. METHODS:BMhTERT cells were treated with VEGF or PlGF in the presence or absence of antioxidan...
journal_title：Journal of vascular research
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doi：10.1159/000226228
更新日期：2009-01-01 00:00:00
abstract：:Dynamic visualization of the intravascular events leading to the extravasation of leukocytes into tissues by intravital microscopy has significantly expanded our understanding of the underlying molecular processes. In contrast, the detailed observation of leukocyte transendothelial and interstitial migration in vivo h...
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更新日期：2003-09-01 00:00:00
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更新日期：2013-01-01 00:00:00
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更新日期：2010-01-01 00:00:00
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更新日期：2021-01-27 00:00:00
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更新日期：2018-01-01 00:00:00
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