Abstract:
OBJECTIVE:The aim of the study was to assess the rat pial microvessel alterations due to transient bilateral common carotid artery occlusion (BCCAO) and to investigate the mechanism of 10% hypertonic glycerol neuroprotection. Our suggestion was that 10% glycerol solution infusion could dilate pial arterioles through nitric oxide release and/or stimulation of ATP-sensitive potassium (K(ATP)) channels. Therefore, we studied the effects of hypertonic glycerol after inhibition of nitric oxide synthase, with N(G)-nitro-L-arginine-methyl ester or N(G)-nitro-L-arginine, or K(ATP) channels with glibenclamide. METHODS:Pial microcirculation of male Wistar rats was visualized by a fluorescent microscopy technique through an open cranial window, using fluorescein isothiocyanate bound to dextran (molecular weight 70 kDa). BCCAO was induced for 30 min and reperfusion lasted 60 min. The arterioles were classified according to the Strahler ordering scheme. Permeability increase was quantified by normalized grey levels (NGL). Leucocytes were stained with rhodamine 6G. Perfused capillary length and capillary red blood cell (RBC) velocity were measured by computer-assisted methods. RESULTS:The arterioles were assigned 5 orders of branchings, from order 1 (diameter 16.0 +/- 2.5 microm) to order 5 (62.0 +/- 5.0 microm). BCCAO caused inhomogenous changes in diameter of arterioles and leakage of fluorescent dextran, that was further enhanced by reperfusion (0.45 +/- 0.05 NGL, p < 0.01). Adhesion of leukocytes to venules was marked and capillary perfusion was reduced by 39.2 +/- 6.0% of baseline as well as capillary RBC velocity. 10% glycerol solution caused an increase in diameter of all arterioles within 25 +/- 2 min of administration (by 20 +/- 5% in order 4, 25 +/- 4% in order 3 and 18 +/- 3% in order 2; p < 0.01). Leakage (0.19 +/- 0.03 NGL, p < 0.01), leukocyte adhesion (2.0 +/- 1.0/100 microm of venular length, p < 0.01) and capillary occlusion (reduction by 13.0 +/- 5.5% of baseline) were prevented compared with controls. Capillary RBC velocity increased compared with controls. N(G)-nitro-L-arginine-methyl ester or N(G)-nitro-L-arginine infused prior to glycerol caused vasoconstriction and reduced the protective effects of hypertonic glycerol on permeability increase. The number of adherent leukocytes and perfused capillary length decreased, while capillary RBC velocity was higher than baseline. Glibenclamide prior to 10% glycerol solution blunted glycerol-induced vasodilatation, but did not affect protection by hypertonic glycerol on blood-brain barrier disruption, leukocyte adhesion and capillary perfusion, preserving high capillary RBC velocity. Papaverine (20 mg/kg body weight) induced an increase in arteriolar diameter, enhancing interstitial edema; adhesion of leukocytes was marked as well as capillary occlusion, while capillary RBC velocity increased. CONCLUSIONS:10% glycerol solution was able to prevent microvascular alterations due to BCCAO protecting cerebral tissue. The effects appear to be due to hyperosmolality causing stimulation of K(ATP) channels, increase in vessel wall shear stress and release of nitric oxide.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Lapi D,Marchiafava PL,Colantuoni Adoi
10.1159/000109818subject
Has Abstractpub_date
2008-01-01 00:00:00pages
89-102issue
2eissn
1018-1172issn
1423-0135pii
000109818journal_volume
45pub_type
杂志文章abstract::Mesenteric endothelial cell function and immunoreactivity for substance P and nitric oxide synthase (NOS) were examined in control rats and rats treated with capsaicin as neonates to destroy primary afferent nerves. Endothelial vasodilator function was examined pharmacologically in the methoxamine raised-tone isolated...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159106
更新日期:1995-09-01 00:00:00
abstract::The effects of the close arterial infusion of histamine upon the microcirculation of facial and nasal tissues were examined in dogs. Blood flow through arteriovenous anastomoses (AVA flow), capillaries (CAP flow) and collaterals (COL flow) were determined by electromagnetic flowmetry and the tracer-microsphere techniq...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000158972
更新日期:1993-01-01 00:00:00
abstract::The role of leukocytes (WBCs) and platelets (PLTs) in the pulmonary circulation may be important in the development of monocrotaline (MCT)-induced pulmonary hypertension in rats. We investigated the suppressive effects of long-term prostaglandin E(1) (PGE(1)) aerosol on the changes in WBCs and PLTs in the peripheral b...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000048996
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND:Lipoxin A4 (LXA4) is a biologically active product generated from arachidonic acid by lipoxygenase action. The production of lipoxins is enhanced by aspirin through acetylation of cyclooxygenase-2, via a mechanism known as 'aspirin-triggered lipoxin'. LXA4 has both anti-inflammatory and proinflammatory actio...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000371490
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND/AIMS:Endothelial nitric oxide synthase (eNOS) is associated with caveolin-1 (Cav-1) in plasma membrane. We tested the hypothesis that eNOS activation by shear stress in resistance vessels depends on synchronized phosphorylation, dissociation from Cav-1 and translocation of the membrane-bound enzyme to Golgi ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000355301
更新日期:2013-01-01 00:00:00
abstract::Topical application of elastase to induce arterial aneurysm formation is an emerging murine model of vascular disease. In the context of aortic abdominal aneurysm (AAA), angiotensin II infusion and porcine pancreatic elastase perfusion models are commonly used today. This study, therefore, compares matrix remodeling, ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000447263
更新日期:2016-01-01 00:00:00
abstract::Protein localization in endothelial cells is tightly regulated to create distinct signaling domains within their tight spatial restrictions including luminal membranes, abluminal membranes, and interendothelial junctions, as well as caveolae and calcium signaling domains. Protein localization in endothelial cells is a...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000512618
更新日期:2021-01-27 00:00:00
abstract:BACKGROUND/AIMS:Endoplasmic reticulum (ER) stress has emerged as a potential mechanism contributing to diabetes and its comorbidities. However, the importance of ER stress in diabetic vascular dysfunction is unclear. The purpose of this study was to examine the effects of the ER stress inhibitor, tauroursodeoxycholic a...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000479967
更新日期:2017-01-01 00:00:00
abstract::Most virus-infected cells release interferon-beta (IFN-beta) as a powerful inducer of antiviral defense. Endothelial cells tightly regulate local immune cell recruitment by expression of adhesion molecules and chemokines. Here, we studied the transcriptional regulation of IFN-beta-induced chemokine expression in prima...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000097977
更新日期:2007-01-01 00:00:00
abstract:OBJECTIVES:Extracellular nucleotide release at the site of arterial injury mediates the proliferation and migration of vascular smooth muscle cells. Our aim was to investigate the role of the P2Y2 nucleotide receptor (P2Y2R) in neointimal hyperplasia. Approach and Results: Vascular injury was induced by the implantatio...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000449059
更新日期:2016-01-01 00:00:00
abstract::We evaluated the effects of a simulated workday of prolonged sitting on blood pressure (BP) and pulse wave velocity (PWV) and examined whether posture (seated vs. supine) affected responses. Participants (n = 25) were adults, with overweight/obesity and elevated BP, and performed seated desk work for 7.5 h. BP and PWV...
journal_title:Journal of vascular research
pub_type: 杂志文章,随机对照试验
doi:10.1159/000510294
更新日期:2020-01-01 00:00:00
abstract::During the early stage of angiogenesis, neovascular sprouts are composed primarily of endothelial cells. As they mature, microvessels acquire a coating of mural cells, which are critical for the development and maintenance of a functional vasculature. Though growth factor regulation of mural cell recruitment has been ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000070711
更新日期:2003-03-01 00:00:00
abstract::The purpose of the present study was to develop a rapid, reproducible method of nonviral gene transfer to the intact vasculature. Male Sprague-Dawley rats were anesthetized, a midline abdominal incision was made and segmental branches of the superior mesenteric artery were dissected free of surrounding mesentery. A sp...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025753
更新日期:2000-09-01 00:00:00
abstract::Although most physiologically important processes are regulated through negative feedback loops and numerous factors regulating endothelial apoptosis are identified, little is known about negative feedback mechanisms for endothelial apoptosis. Here we describe the release of soluble anti-apoptotic activity by endothel...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000111072
更新日期:2008-01-01 00:00:00
abstract::In coronary artery diseases, cigarette smoking is a risk factor and the endothelin system plays a key role in the pathogenesis. This study was to examine if dimethylsulfoxide-soluble smoke particles (DSP) upregulate endothelin type-B (ETB) receptors in the coronary artery and investigate the mechanism. The isolated ra...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000350866
更新日期:2013-01-01 00:00:00
abstract::Actin cytoskeleton reorganization is regulated by various actin-binding proteins. Cofilin is the principal filament-depolymerizing protein, whose activity is reduced upon phosphorylation by LIMK. Thus, LIMK and cofilin comprise a signal transduction module regulating actin turnover and myogenic tone in healthy vascula...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000081247
更新日期:2004-09-01 00:00:00
abstract::The goal of this study was to determine the role of endothelium-derived relaxing factor (EDRF) in dilatation of cerebral arterioles in response to adenosine 5'-diphosphate (ADP). Using intravital microscopy, we measured the diameters of pial arterioles in vivo in rats during superfusion with ADP, acetylcholine and nit...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000158951
更新日期:1992-09-01 00:00:00
abstract::We tested the hypothesis that oxidized low-density lipoprotein (oxLDL)-induced inactivation of Akt within endothelial progenitor cells (EPCs) is mediated at the level of phosphoinositide 3-kinase (PI3K), specifically by nitrosylation of the p85 subunit of PI3K, and that this action is critical in provoking oxLDL-induc...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000313879
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:We have developed an in vitro model by organ culture of rat mesenteric arteries to imitate vascular smooth muscle cell (VSMC) receptor changes in cardiovascular disease. By using this model, alteration of VSMC thromboxane A(2) (TP) receptors was studied. METHODS AND RESULTS:After organ culture of the arteri...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000153247
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND/AIMS:The endothelium has been recognized as a key component in the regulation of blood vessels. We designed a simple procedure to separate endothelial and smooth muscle RNA from rat aorta and mesenteric artery and used this method to establish the distribution of Na(+)/K(+)-ATPase alpha-subunit isoforms (NaK...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000095963
更新日期:2006-01-01 00:00:00
abstract:OBJECTIVES:Hyperuricemia provokes endothelial dysfunction (ECD). Decreased endothelial nitric oxide synthase (eNOS) activity is an important source of ECD. Cationic amino acid transporter-1 (CAT-1) is the specific arginine transporter for eNOS. We hypothesize that hyperuricemia inhibits arginine uptake. METHODS:Experi...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000320356
更新日期:2011-01-01 00:00:00
abstract:PURPOSE:To determine: (1) whether variables of a pulse wave form analysis of ocular microcirculation shown by laser speckle flowgraphy (LSFG) correlate with left ventricular (LV) systolic dysfunction and (2) whether these variables correlate with LV diastolic dysfunction in subjects without LV systolic dysfunction as a...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000494066
更新日期:2018-01-01 00:00:00
abstract::Recent research in ultrasound technology has led to the development of a high-resolution echo-tracking device. The present study was performed to evaluate the accuracy in the measurement of human radial artery intima-media thickness with this new device. We determined the correlation between histological and ultrasoni...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159037
更新日期:1994-03-01 00:00:00
abstract::The Cytoscan Model E-II (Cytometrics Inc., Philadelphia, Pa., USA) is a newly developed instrument which functions as an intravital microscope and is small and easily portable. Through the use of orthogonal polarization spectral (OPS) imaging, the Cytoscan Model E-II delivers images of the microcirculation which are c...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000054079
更新日期:2000-11-01 00:00:00
abstract::The effect of shear stress at the endothelium in the attenuation of the noradrenaline-induced constriction of the femoral vascular bed perfused at a constant blood flow was investigated in 16 anesthetized cats. It is known that the adrenergic vasoconstriction of the femoral vascular bed is considerably greater at a co...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000097750
更新日期:2007-01-01 00:00:00
abstract:PURPOSE:Exercise elicits early adaptation of coronary vessels enabling the coronary circulation to respond adequately to higher flow demands. We hypothesized that short-term daily exercise induces biomechanical and functional remodeling of the coronary resistance arteries related to pressure. METHODS:Male rats were su...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000486571
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Extracellular matrix deposition is the main factor inducing stenotic lesions in arterial grafts. Lysyl oxidases (LOX) play a key role in stabilizing collagen and elastin. OBJECTIVE:To examine the repair response to arterial allografts in terms of LOX expression and collagen/elastin deposition using LOX inhi...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000317399
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Hypoxia results in pulmonary hypertension and vascular remodeling due to induction of pulmonary artery cell proliferation. Besides pulmonary artery smooth muscle cells, pulmonary artery endothelial cells (PAECs) are also involved in the development of pulmonary hypertension, but the effect of hypoxia on PAEC...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000327005
更新日期:2011-01-01 00:00:00
abstract::Ruptured atherosclerotic plaques, lined with activated platelets, constitute an attractive target for magnetic resonance imaging (MRI). This study evaluated whether microparticles of iron oxide (MPIO) targeting ligand-induced binding sites (LIBS) on the activated conformation of glycoprotein IIb/IIIa could be used to ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000135660
更新日期:2009-01-01 00:00:00
abstract::The pathophysiology of Raynaud's phenomenon (RP) remains an enigma. Whatever theories proposed, the final event leading to the clinical symptoms is the occlusion of digital vessels. However, the possibility that the upstream large arterial vessels contribute to vasopasm has never been investigated. We used a high reso...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159237
更新日期:1997-07-01 00:00:00