Abstract:
:The role of leukocytes (WBCs) and platelets (PLTs) in the pulmonary circulation may be important in the development of monocrotaline (MCT)-induced pulmonary hypertension in rats. We investigated the suppressive effects of long-term prostaglandin E(1) (PGE(1)) aerosol on the changes in WBCs and PLTs in the peripheral blood and the pulmonary microvasculature during the development of chronic pulmonary hypertension in MCT rats by real-time confocal scanning laser microscopy. The number of WBCs and PLTs in peripheral blood did not significantly change by inhalation of PGE(1). The number of WBCs and PLTs sequestered in the pulmonary microvasculature of rats subjected PGE(1) inhalation immediately after MCT injection rats with (MCT plus PGE(1) inhalation) significantly decreased from 1 to 4 weeks compared to rats not subjected to PGE(1) inhalation (p < 0.01). The pulmonary systolic arterial pressure and the weight ratio of the right ventricle to the intraventricular septum plus the left ventricle (RV/IVS + LV weight ratio or RV weight ratio) in rats with MCT plus PGE(1) inhalation significantly decreased compared to rats not subjected to PGE(1) inhalation (p < 0.01). The levels of peripheral CD62L-positive WBCs in rats with MCT plus PGE(1) inhalation significantly decreased from 1 to 2 weeks compared to rats not subjected to PGE(1) inhalation, but the levels of peripheral CD18 and CD49d-positive WBCs did not significantly change. We conclude that long-term inhalation of PGE(1) is a very useful therapy in chronic pulmonary hypertension, and the mechanism of suppressing pulmonary hypertension is associated with suppressive effects on sequestered WBCs, especially CD62-positive WBCs and PLTs in the pulmonary microvasculature.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Kato S,Kishiro I,Sugimura H,Machida S,Fuse D,Kaneko Ndoi
10.1159/000048996keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
83-7issue
1eissn
1018-1172issn
1423-0135pii
48996journal_volume
39pub_type
杂志文章abstract::The present study investigated transcellular signalling mechanism involved in thrombin-induced production of plasminogen activator inhibitor-1 (PAI-1) in cultured vascular baboon aortic smooth muscle cells (BASMC). Treatments with thrombin dose-dependently increased the steady state levels of PAI-1 mRNA and the genera...
journal_title:Journal of vascular research
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journal_title:Journal of vascular research
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