Abstract：
:The circadian clock is rhythmically expressed in blood vessels, but the interaction between the circadian clock and disturbed blood flow remains unclear. We examined the relationships between BMAL1 and CLOCK and 2 regulators of endothelial function, AKT1 and endothelial nitric oxide synthase (eNOS), in vascular regions of altered blood flow. We found that the aortic arch from WT mice exhibited reduced sensitivity to acetylcholine (Ach)-mediated relaxation relative to the thoracic aorta. In Clock-mutant (mut) mice the aorta exhibited a reduced sensitivity to Ach. In WT mice, the phosphorylated forms of eNOS and AKT were decreased in the aortic arch, while BMAL1 and CLOCK expression followed a similar pattern of reduction in the arch. In conditions of surgically induced flow reduction, phosphorylated-eNOS (serine 1177) increased, as did p-AKT in the ipsilateral left common carotid artery (LC) of WT mice. Similarly, BMAL1 and CLOCK exhibited increased expression after 5 days in the remodeled LC. eNOS expression was increased at 8 p.m. versus 8 a.m. in WT mice, and this pattern was abolished in mut and Bmal1-KO mice. These data suggest that the circadian clock may be a biomechanical and temporal sensor that acts to coordinate timing, flow dynamics, and endothelial function.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Shang X,Pati P,Anea CB,Fulton DJ,Rudic RDdoi
10.1159/000452410subject
Has Abstractpub_date
2016-01-01 00:00:00pages
269-278issue
5-6eissn
1018-1172issn
1423-0135pii
000452410journal_volume
53pub_type
杂志文章abstract：:The proliferation of vascular smooth muscle cells (VSMC) plays a significant part in both the developing atherosclerotic lesion and in restenosis. Heparin has been widely reported to inhibit the growth of VSMC in culture and intimal VSMC in some animal models of vascular hyperplasia. Clinical trials with heparin, howe...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025616
更新日期：1998-11-01 00:00:00
abstract：:Topical application of elastase to induce arterial aneurysm formation is an emerging murine model of vascular disease. In the context of aortic abdominal aneurysm (AAA), angiotensin II infusion and porcine pancreatic elastase perfusion models are commonly used today. This study, therefore, compares matrix remodeling, ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000447263
更新日期：2016-01-01 00:00:00
abstract：:Increased vascular disease occurs with low albumin (human serum albumin, HSA), possibly reflecting specific inhibition of endothelial apoptosis reported for tissue culture. Despite the reported specificity for endothelial protection by HSA, the high but physiological concentrations needed appear more consistent with n...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000101777
更新日期：2007-01-01 00:00:00
abstract：BACKGROUND/AIMS:Endoplasmic reticulum (ER) stress has emerged as a potential mechanism contributing to diabetes and its comorbidities. However, the importance of ER stress in diabetic vascular dysfunction is unclear. The purpose of this study was to examine the effects of the ER stress inhibitor, tauroursodeoxycholic a...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000479967
更新日期：2017-01-01 00:00:00
abstract：BACKGROUND/AIMS:Endothelial nitric oxide synthase (eNOS) is associated with caveolin-1 (Cav-1) in plasma membrane. We tested the hypothesis that eNOS activation by shear stress in resistance vessels depends on synchronized phosphorylation, dissociation from Cav-1 and translocation of the membrane-bound enzyme to Golgi ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000355301
更新日期：2013-01-01 00:00:00
abstract：OBJECTIVES:Extracellular nucleotide release at the site of arterial injury mediates the proliferation and migration of vascular smooth muscle cells. Our aim was to investigate the role of the P2Y2 nucleotide receptor (P2Y2R) in neointimal hyperplasia. Approach and Results: Vascular injury was induced by the implantatio...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000449059
更新日期：2016-01-01 00:00:00
abstract：:The contribution of ATP-sensitive potassium channel (KATP channel) blockade in the vasoconstriction produced by vasopressin was studied. All experiments were performed using rat thoracic aorta cut in 4-mm rings, denuded from their endothelium and mounted into 20-ml organ baths. Glibenclamide (0.01-10 microM), a KATP c...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159088
更新日期：1995-03-01 00:00:00
abstract：:In response to stimuli, endothelial cells release arachidonic acid, a lipid precursor of various vasoactive substances. We have investigated the relationships between cytosolic Ca2+ movements and arachidonic acid release in human umbilical vein endothelial cells. Histamine, a receptor-dependent agonist, and thapsigarg...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025647
更新日期：1999-05-01 00:00:00
abstract：:Insulin was reported to decrease Na(+)/H(+) exchange activity in murine vascular smooth muscle (VSM) tissue. In most other cells, insulin increases activity. We tested the effects of insulin on Na(+)/H(+) exchange activity in primary cultured canine VSM cells. Intracellular pH (pH(i)) was measured with 2',7'-bis(2-car...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000329587
更新日期：2011-01-01 00:00:00
abstract：:The mechanosensitive nonselective cation channel (NSC(MS)) is a key player in vascular myogenic contraction. The functional channel density and pressure sensitivity of NSC(MS) in vascular myocytes were compared between pulmonary and systemic arteries (coronary, mesenteric and cerebral arteries) in the rabbit. In cell-...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000093607
更新日期：2006-01-01 00:00:00
abstract：:Proper development of the vascular system as one of the earliest and most critical steps during vertebrate embryogenesis is ensured by the exact spatial and temporal control of gene expression in cells forming the vessel network. Whereas the regulation of vascular system development is well elucidated on the level of ...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000353287
更新日期：2013-01-01 00:00:00
abstract：:The aim of this study was to verify that subcutaneous lymph node transplantation inducing lymphatic regeneration is possible in healthy adult rats, in analogy to results obtained in other species. This rat model was used to determine the effects of lymph node fragmentation as well as sheep erythrocytes and platelet-ri...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000194269
更新日期：2009-01-01 00:00:00
abstract：BACKGROUND:Extracellular matrix deposition is the main factor inducing stenotic lesions in arterial grafts. Lysyl oxidases (LOX) play a key role in stabilizing collagen and elastin. OBJECTIVE:To examine the repair response to arterial allografts in terms of LOX expression and collagen/elastin deposition using LOX inhi...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000317399
更新日期：2011-01-01 00:00:00
abstract：:We evaluated the effects of a simulated workday of prolonged sitting on blood pressure (BP) and pulse wave velocity (PWV) and examined whether posture (seated vs. supine) affected responses. Participants (n = 25) were adults, with overweight/obesity and elevated BP, and performed seated desk work for 7.5 h. BP and PWV...
journal_title：Journal of vascular research
pub_type： 杂志文章,随机对照试验
doi：10.1159/000510294
更新日期：2020-01-01 00:00:00
abstract：BACKGROUND:The aim of the present study was to evaluate different signaling pathways by which exercise training would interfere in endothelial function in obesity. Therefore, we examined adipocytokine levels and their receptors in the corpus cavernosum and femoral artery from trained rats on a high-fat diet. METHODS:F...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000457800
更新日期：2017-01-01 00:00:00
abstract：OBJECTIVE:Increased peripheral arterial resistances and decreased maximum vasodilation are characteristic features of chronic hypertension. However, little data are available in the literature regarding the possible alterations in the temporal patterns of vasodilatory responses elicited by various stimuli. DESIGN:This...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000090944
更新日期：2006-01-01 00:00:00
abstract：:Signaling pathways activated by the tachykinin substance P (SP) were investigated in pig coronary artery endothelial cells (PCAECs). Single cells were obtained after enzymatic digestion of coronary arteries. Intracellular Ca2+ ([Ca2+]i) was measured from fura-2 fluorescence while membrane potential or ionic current wa...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159222
更新日期：1997-05-01 00:00:00
abstract：:The pH has marked effects on the blood flow in several vascular beds but the underlying mechanisms remain incompletely understood. It is still not agreed, for example, whether it is the fall in extracellular pH or intracellular pH that is responsible for changes in tone resulting from hypercapnic acidosis. The issue h...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000159163
更新日期：1996-09-01 00:00:00
abstract：:Vascular restenosis is one of the major concerns for the management of coronary artery disease using therapeutic vascular procedures. Treatments with thrombin-specific inhibitors, hirudin or hirulog-1, reduced ischemic events in coronary artery disease patients. Early started and prolonged infusions of these thrombin ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000051041
更新日期：2001-03-01 00:00:00
abstract：:During the early stage of angiogenesis, neovascular sprouts are composed primarily of endothelial cells. As they mature, microvessels acquire a coating of mural cells, which are critical for the development and maintenance of a functional vasculature. Though growth factor regulation of mural cell recruitment has been ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000070711
更新日期：2003-03-01 00:00:00
abstract：:Thrombospondin-1 (TSP1) is a potent natural inhibitor of angiogenesis. Although TSP1 has been reported to induce endothelial cell apoptosis in vitro and to downregulate neovascularization in vivo, the molecular mechanisms that link these two processes have yet to be established. Here we report that TSP1 mediates endot...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025733
更新日期：2000-05-01 00:00:00
abstract：:Endothelin-1 (ET-1), a vasoconstrictor peptide produced by endothelial and vascular smooth muscle cells (VSMC) might play a role in vascular remodelling. To investigate the proposed 'mitogenic' potential of ET-1, we examined the effects of chronic exposure of VSMC to ET-1 on cell cycle, growth/proliferation and differ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000158994
更新日期：1993-07-01 00:00:00
abstract：BACKGROUND:We have developed an in vitro model by organ culture of rat mesenteric arteries to imitate vascular smooth muscle cell (VSMC) receptor changes in cardiovascular disease. By using this model, alteration of VSMC thromboxane A(2) (TP) receptors was studied. METHODS AND RESULTS:After organ culture of the arteri...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000153247
更新日期：2009-01-01 00:00:00
abstract：:Migration of vascular smooth muscle cells (SMCs) leading to neointimal hyperplasia is an early and cardinal feature of atherogenesis. Migration of rat aortic SMCs from an upper chamber towards a lower one has been studied in a microchemotaxis (Boyden) chamber. Spontaneous migration of SMCs was practically prevented by...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025580
更新日期：1998-05-01 00:00:00
abstract：:There is evidence suggesting that fluid shear stress activates phospholipid turnover in endothelial cells, but it is not clear which phospholipids are involved in the transduction of the flow signal. Cultured human umbilical-vein endothelial cells were prelabeled with [14C]-arachidonic acid and subjected to laminar sh...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000158963
更新日期：1992-11-01 00:00:00
abstract：:Most virus-infected cells release interferon-beta (IFN-beta) as a powerful inducer of antiviral defense. Endothelial cells tightly regulate local immune cell recruitment by expression of adhesion molecules and chemokines. Here, we studied the transcriptional regulation of IFN-beta-induced chemokine expression in prima...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000097977
更新日期：2007-01-01 00:00:00
abstract：:Atherosclerosis underlies late occlusion of human saphenous vein (HSV) coronary artery bypass grafts. Monocyte infiltration is implicated, but its mechanisms are unclear given that HSV normally expresses the ICAM-1 but not the VCAM-1 adhesion molecule. To define the mechanisms underlying monocyte adhesion, samples of ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000063687
更新日期：2002-05-01 00:00:00
abstract：BACKGROUND:Hypoxia results in pulmonary hypertension and vascular remodeling due to induction of pulmonary artery cell proliferation. Besides pulmonary artery smooth muscle cells, pulmonary artery endothelial cells (PAECs) are also involved in the development of pulmonary hypertension, but the effect of hypoxia on PAEC...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000327005
更新日期：2011-01-01 00:00:00
abstract：PURPOSE:Exercise elicits early adaptation of coronary vessels enabling the coronary circulation to respond adequately to higher flow demands. We hypothesized that short-term daily exercise induces biomechanical and functional remodeling of the coronary resistance arteries related to pressure. METHODS:Male rats were su...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000486571
更新日期：2018-01-01 00:00:00
abstract：:Tumor necrosis factor (TNF) receptor-associated factors (TRAFs) are cytoplasmic adaptor proteins of the TNF/interleukin (IL)-1/Toll-like receptor superfamily. Ligands of this family such as TNFα, CD40L, and IL-1β promote chronic inflammatory processes such as atherosclerosis and restenosis, the latter being a common a...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000501615
更新日期：2019-01-01 00:00:00
