Abstract:
:The proliferation of vascular smooth muscle cells (VSMC) plays a significant part in both the developing atherosclerotic lesion and in restenosis. Heparin has been widely reported to inhibit the growth of VSMC in culture and intimal VSMC in some animal models of vascular hyperplasia. Clinical trials with heparin, however, have failed to inhibit restenosis following angioplasty. Bovine serum is normally used as a growth supplement in in vitro VSMC growth assays. We have compared the effects of human serum with those of bovine serum on the cellular response to heparin in human VSMC culture. While heparin inhibited the proliferation of human VSMC in the presence of bovine serum, it was totally ineffective in the presence of human serum. These observations were consistent over a wide range of serum and VSMC samples. Experiments utilizing neutralizing antibodies to a number of growth factors showed that cells in either serum were similarly dependent on platelet-derived growth factor for proliferation. In contrast, proliferation in the presence of bovine serum was shown to be dependent on extracellular basic fibroblast growth factor, whereas that in human serum was not. Direct binding of [3H]-heparin to VSMC was significantly reduced in the presence of human serum compared with bovine serum, and the former contained twice the concentration of heparin-binding factors of the latter. Removal of heparin-binding factors from either serum type significantly reduced the proliferation potential. Fractionation of heparin-binding factors from human serum showed that the major growth-promoting activity, together with heparin resistance, was contained within a fraction excluded by a 100,000 molecular weight membrane. We conclude that the mechanism of resistance to heparin in human serum is likely to be due to a combination of differential growth factor binding and interference with heparin interaction with cellular receptors by a high molecular weight heparin-binding factor. This phenomenon may significantly contribute to the lack of success of heparin as an antirestenotic agent in clinical trials.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Underwood PA,Mitchell SM,Whitelock JMdoi
10.1159/000025616subject
Has Abstractpub_date
1998-11-01 00:00:00pages
449-60issue
6eissn
1018-1172issn
1423-0135pii
jvr35449journal_volume
35pub_type
杂志文章abstract:UNLABELLED:Nitric oxide (NO) and cyclooxygenase (COX) derived prostaglandins (PGs) are involved in vascular homeostasis. Contradictory results have been obtained in previous studies on the putative 'cross-talk' between these two pathways. Our aim was to evaluate the interaction between NO and PG release in human microv...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000074550
更新日期:2003-09-01 00:00:00
abstract::Chemokines play an important role in the process of leukocyte recruitment and activation at sites of inflammation. Until recently, the actions of chemokines and the expression of their receptors have only been described on different leukocyte populations. However, increasing evidence has suggested that non-haematopoie...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000025707
更新日期:2000-01-01 00:00:00
abstract::Insulin was reported to decrease Na(+)/H(+) exchange activity in murine vascular smooth muscle (VSM) tissue. In most other cells, insulin increases activity. We tested the effects of insulin on Na(+)/H(+) exchange activity in primary cultured canine VSM cells. Intracellular pH (pH(i)) was measured with 2',7'-bis(2-car...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000329587
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVES:Hyperuricemia provokes endothelial dysfunction (ECD). Decreased endothelial nitric oxide synthase (eNOS) activity is an important source of ECD. Cationic amino acid transporter-1 (CAT-1) is the specific arginine transporter for eNOS. We hypothesize that hyperuricemia inhibits arginine uptake. METHODS:Experi...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000320356
更新日期:2011-01-01 00:00:00
abstract:UNLABELLED:Diabetic patients have an increased risk of restenosis and late stent thrombosis after angioplasty, i.e. complications that are related to a defective re-endothelialization. Exendin-4, a stable glucagon-like peptide (GLP)-1 receptor agonist, has been suggested to influence the formation of intimal hyperplasi...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000381097
更新日期:2015-01-01 00:00:00
abstract::The purpose of the present study was to develop a rapid, reproducible method of nonviral gene transfer to the intact vasculature. Male Sprague-Dawley rats were anesthetized, a midline abdominal incision was made and segmental branches of the superior mesenteric artery were dissected free of surrounding mesentery. A sp...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025753
更新日期:2000-09-01 00:00:00
abstract::Dietary flavonoids are thought to protect against cardiovascular disease. We have studied the effects of bioactive isoflavone metabolites on hyperlipidemia, endothelial dysfunction and the development of atherosclerotic lesions in apolipoprotein E-deficient (apoE(0)) mice fed a Western high-fat diet. Supplementation w...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000071891
更新日期:2003-05-01 00:00:00
abstract::Intima-media thickening (IMT) of the carotid artery, a form of vascular remodeling, correlates well with coronary artery disease risk in humans. Vascular remodeling in response to blood flow is a complex process that critically involves altered cell matrix interactions. To gain insight into these events, we performed ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000081804
更新日期:2004-11-01 00:00:00
abstract::Peroxynitrite is an endothelium-independent vasodilator that induces relaxation via membrane hyperpolarization. The activation of IP3 receptors triggers the opening of potassium channels and hyperpolarization. Previously we found that relaxation to peroxynitrite was maintained during the development of atherosclerosis...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000461581
更新日期:2017-01-01 00:00:00
abstract::Organic nitrates act as vasodilators and have long been used for treatment of cardiovascular diseases. However, the therapeutic effect of nitrates is limited by induction of nitrate tolerance which is associated with endothelial dysfunction and enhanced vasoconstriction. Multiple mechanisms cause nitrate tolerance inc...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000065542
更新日期:2002-07-01 00:00:00
abstract::The novel endothelial cell tyrosine kinase receptors, Tie1 and Tie2, are essential for vascular development and remodeling in the embryo but little is known regarding the regulation of their expression and their role in the maintenance and repair of the adult vascular system. We examined the expression of Tie1 and Tie...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025655
更新日期:1999-07-01 00:00:00
abstract::Ruptured atherosclerotic plaques, lined with activated platelets, constitute an attractive target for magnetic resonance imaging (MRI). This study evaluated whether microparticles of iron oxide (MPIO) targeting ligand-induced binding sites (LIBS) on the activated conformation of glycoprotein IIb/IIIa could be used to ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000135660
更新日期:2009-01-01 00:00:00
abstract::The Ig-like cell adhesion molecule ICAM-3 is mainly expressed on human leukocytes and is involved in cell-cell interactions. Its expression on endothelium is observed during disorders such as Crohn's disease and in solid tumors. We found low but detectable expression of ICAM-3 on VE-cadherin-expressing cells from prim...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000076126
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:It has been reported that smoking is one of the strongest positive risk factors for abdominal aortic aneurysms (AAAs). Although many studies have been directed to decipher the effect of smoking on AAA, its effect on macrophage activation has not yet been explored. OBJECTIVES:We have reported the importance ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000498893
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:Infusion of exogenous vascular endothelial growth factor (VEGF) into adult brain at doses above 60 ng/day induces dramatic angiogenesis accompanied by vascular leak and inflammation. Blood vessels formed by this treatment are dilated and tortuous, exhibiting a pathological morphology. Pathological VEGF-induc...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000101450
更新日期:2007-01-01 00:00:00
abstract::Endothelin-1 (ET-1), a vasoconstrictor peptide produced by endothelial and vascular smooth muscle cells (VSMC) might play a role in vascular remodelling. To investigate the proposed 'mitogenic' potential of ET-1, we examined the effects of chronic exposure of VSMC to ET-1 on cell cycle, growth/proliferation and differ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000158994
更新日期:1993-07-01 00:00:00
abstract::We investigated the possible interference of smooth muscle cells with monocyte response to LDL as well as with their adhesion and transmigration in a coculture of porcine endothelial and smooth muscle cells. Lysophosphatidylcholine (LPC), a component of oxidized LDL (oxLDL), stimulated the adhesion of THP-1 cells to e...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000051081
更新日期:2001-09-01 00:00:00
abstract::The role of leukocytes (WBCs) and platelets (PLTs) in the pulmonary circulation may be important in the development of monocrotaline (MCT)-induced pulmonary hypertension in rats. We investigated the suppressive effects of long-term prostaglandin E(1) (PGE(1)) aerosol on the changes in WBCs and PLTs in the peripheral b...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000048996
更新日期:2002-01-01 00:00:00
abstract::The effects of the close arterial infusion of histamine upon the microcirculation of facial and nasal tissues were examined in dogs. Blood flow through arteriovenous anastomoses (AVA flow), capillaries (CAP flow) and collaterals (COL flow) were determined by electromagnetic flowmetry and the tracer-microsphere techniq...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000158972
更新日期:1993-01-01 00:00:00
abstract::Recent research in ultrasound technology has led to the development of a high-resolution echo-tracking device. The present study was performed to evaluate the accuracy in the measurement of human radial artery intima-media thickness with this new device. We determined the correlation between histological and ultrasoni...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159037
更新日期:1994-03-01 00:00:00
abstract::Studies on the possible causal relationship between the Ca2+ channel current density in the vascular muscle cell (VMC) and increases in blood pressure were extended by a comparison of stroke-prone spontaneously hypertensive rats (SP-SHR) with N/nih outbred normotensive rats. Maximal amplitudes of both L-type and T-typ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159064
更新日期:1994-11-01 00:00:00
abstract::Signaling pathways activated by the tachykinin substance P (SP) were investigated in pig coronary artery endothelial cells (PCAECs). Single cells were obtained after enzymatic digestion of coronary arteries. Intracellular Ca2+ ([Ca2+]i) was measured from fura-2 fluorescence while membrane potential or ionic current wa...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159222
更新日期:1997-05-01 00:00:00
abstract:BACKGROUND/AIMS:Endothelial nitric oxide synthase (eNOS) is associated with caveolin-1 (Cav-1) in plasma membrane. We tested the hypothesis that eNOS activation by shear stress in resistance vessels depends on synchronized phosphorylation, dissociation from Cav-1 and translocation of the membrane-bound enzyme to Golgi ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000355301
更新日期:2013-01-01 00:00:00
abstract::Therapies aimed at manipulating the microcirculation require the ability to control angiogenesis, defined as the sprouting of new capillaries from existing vessels. Blocking angiogenesis would be beneficial in many pathologies (e.g. cancer, retinopathies and rheumatoid arthritis). In others (e.g. myocardial infarction...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000362276
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:Increased peripheral arterial resistances and decreased maximum vasodilation are characteristic features of chronic hypertension. However, little data are available in the literature regarding the possible alterations in the temporal patterns of vasodilatory responses elicited by various stimuli. DESIGN:This...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000090944
更新日期:2006-01-01 00:00:00
abstract::Proper development of the vascular system as one of the earliest and most critical steps during vertebrate embryogenesis is ensured by the exact spatial and temporal control of gene expression in cells forming the vessel network. Whereas the regulation of vascular system development is well elucidated on the level of ...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000353287
更新日期:2013-01-01 00:00:00
abstract::Although the content of histamine in myocardial tissue is high, its contribution to the regulation of coronary blood flow has not been clearly defined. The aim of the present study was to investigate whether or not nitric oxide (NO), an important modulator of coronary vascular tone, is involved in histamine-induced co...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000158987
更新日期:1993-05-01 00:00:00
abstract:OBJECTIVE:It is well known that inflammation plays key roles in the development of atherosclerosis and that the transplantation of bone marrow mononuclear cells (BMMNCs) can suppress inflammation in rodent models of ischemic diseases. Here, we explored whether transplantation of autologous BMMNCs could prevent the prog...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000449201
更新日期:2016-01-01 00:00:00
abstract::It has been well established that wall shear stress is an important determinant of endothelial cell function and gene expression as well as of its structure. There is increasing evidence that low wall shear stress, as present in artery bifurcations opposite to the flow divider where atherosclerotic lesions preferentia...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000091648
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Low birth weight (LBW) is associated with increased risk of type 2 diabetes and cardiovascular disease. We studied endothelial function and insulin sensitivity in young men with LBW (n = 22) and controls (n = 22). METHODS:Insulin sensitivity and endothelial function was studied with venous occlusion plethys...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000235968
更新日期:2010-01-01 00:00:00