Abstract:
:Mesenteric endothelial cell function and immunoreactivity for substance P and nitric oxide synthase (NOS) were examined in control rats and rats treated with capsaicin as neonates to destroy primary afferent nerves. Endothelial vasodilator function was examined pharmacologically in the methoxamine raised-tone isolated perfused mesenteric arterial bed. Endothelial immunoreactivity for substance P and NOS was examined at the ultrastructural level by electron-microscopic immunocytochemistry. The endothelium-dependent vasodilators acetylcholine and adenosine 5'-triphosphate elicited dose-dependent relaxations which were not different between control and capsaicin-treated rats. Dose-dependent relaxations to endothelium-independent vasodilators, calcitonin gene-related peptide and sodium nitroprusside, were also unchanged by capsaicin treatment. Positive staining for substance P was detected in 25% of endothelial cells in both control and capsaicin-treated rats. Positive staining for NOS was detected in 50% of endothelial cells in control rats, and this was not changed by capsaicin treatment. These results confirm that endothelial substance P is independent of substance P contained in sensory nerves. Long-term sensory denervation does not produce changes in endothelium-dependent or -independent relaxation, or in the number of endothelial cells showing positive labelling for substance P and NOS in rat mesenteric arteries.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Ralevic V,Dikranian K,Burnstock Gdoi
10.1159/000159106subject
Has Abstractpub_date
1995-09-01 00:00:00pages
320-7issue
5eissn
1018-1172issn
1423-0135journal_volume
32pub_type
杂志文章abstract::The vascular endothelium is the source of a number of vasodilator and vasoconstrictor autacoids and is thus a key regulator of vascular homeostasis. We studied the effects of altering the balance between protein tyrosine kinase and phosphatase activity on Ca2+ signalling and phosphotyrosine levels in cultured human en...
journal_title:Journal of vascular research
pub_type: 杂志文章
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更新日期:1996-05-01 00:00:00
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journal_title:Journal of vascular research
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journal_title:Journal of vascular research
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更新日期:2002-01-01 00:00:00
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更新日期:2007-01-01 00:00:00
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journal_title:Journal of vascular research
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journal_title:Journal of vascular research
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journal_title:Journal of vascular research
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doi:10.1159/000051041
更新日期:2001-03-01 00:00:00
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journal_title:Journal of vascular research
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更新日期:1995-07-01 00:00:00
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journal_title:Journal of vascular research
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025592
更新日期:1998-07-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
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更新日期:1994-11-01 00:00:00
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更新日期:2016-01-01 00:00:00
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journal_title:Journal of vascular research
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更新日期:1994-03-01 00:00:00
abstract:BACKGROUND:Infusion of exogenous vascular endothelial growth factor (VEGF) into adult brain at doses above 60 ng/day induces dramatic angiogenesis accompanied by vascular leak and inflammation. Blood vessels formed by this treatment are dilated and tortuous, exhibiting a pathological morphology. Pathological VEGF-induc...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000101450
更新日期:2007-01-01 00:00:00
abstract::Insulin was reported to decrease Na(+)/H(+) exchange activity in murine vascular smooth muscle (VSM) tissue. In most other cells, insulin increases activity. We tested the effects of insulin on Na(+)/H(+) exchange activity in primary cultured canine VSM cells. Intracellular pH (pH(i)) was measured with 2',7'-bis(2-car...
journal_title:Journal of vascular research
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journal_title:Journal of vascular research
pub_type: 杂志文章
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更新日期:1993-07-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000095963
更新日期:2006-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025647
更新日期:1999-05-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025599
更新日期:1998-09-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000158963
更新日期:1992-11-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000109818
更新日期:2008-01-01 00:00:00