Abstract：
:Regulation of [Ca2+]i and modulation of the [Ca2+]i sensitivity of myosin phosphorylation in smooth muscles may involve phosphorylation of one or more proteins on tyrosine residues. We tested this hypothesis by measuring tyrosine phosphorylation of proteins extracted from swine carotid artery and separated by SDS gel electrophoresis. Tyrosine phosphorylation was estimated by the binding of antiphosphotyrosine antibodies to proteins in SDS gels. We found four bands with approximate molecular weights of 120, 110, 85 and 75 kD in which tyrosine phosphorylation increased 1 min after histamine stimulation. After washout of histamine, dephosphorylation of the proteins in these four bands occurred at a slower rate than relaxation. Tyrosine phosphorylation of these four protein bands did not correlate with the [Ca2+]i sensitivity of myosin phosphorylation following agonist or high [K+]o stimulation. Phorbol dibutyrate stimulation also induced tyrosine phosphorylation of these four protein bands. These data suggest that tyrosine phosphorylation of the proteins in these four bands may be involved in the initial phase of swine carotid artery contraction. However, there was, at most, only a minor involvement of tyrosine phosphorylation of these four protein bands in the sustained phase of contraction or in the regulation of [Ca2+]i sensitivity of myosin phosphorylation in the swine carotid artery. These data do not rule out a role for other, less abundant tyrosine phosphoproteins in the regulation of sustained contraction or the [Ca2+]i sensitivity of myosin phosphorylation.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Rembold CM,Weaver BAdoi
10.1159/000159196subject
Has Abstractpub_date
1997-01-01 00:00:00pages
1-10issue
1eissn
1018-1172issn
1423-0135journal_volume
34pub_type
杂志文章abstract：:Intima-media thickening (IMT) of the carotid artery, a form of vascular remodeling, correlates well with coronary artery disease risk in humans. Vascular remodeling in response to blood flow is a complex process that critically involves altered cell matrix interactions. To gain insight into these events, we performed ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000081804
更新日期：2004-11-01 00:00:00
abstract：INTRODUCTION:This study aimed to investigate whether epigallocatechin-3-gallate (EGCG) shows antioxidant activity against angiotensin II (Ang II)-induced human umbilical vein endothelial cell (HUVEC) apoptosis. MATERIALS AND METHODS:The viability of HUVECs was revealed by MTT and LDH assay. The cell apoptosis was dete...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000479873
更新日期：2017-01-01 00:00:00
abstract：:We evaluated the effects of a simulated workday of prolonged sitting on blood pressure (BP) and pulse wave velocity (PWV) and examined whether posture (seated vs. supine) affected responses. Participants (n = 25) were adults, with overweight/obesity and elevated BP, and performed seated desk work for 7.5 h. BP and PWV...
journal_title：Journal of vascular research
pub_type： 杂志文章,随机对照试验
doi：10.1159/000510294
更新日期：2020-01-01 00:00:00
abstract：:Vascular restenosis is one of the major concerns for the management of coronary artery disease using therapeutic vascular procedures. Treatments with thrombin-specific inhibitors, hirudin or hirulog-1, reduced ischemic events in coronary artery disease patients. Early started and prolonged infusions of these thrombin ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000051041
更新日期：2001-03-01 00:00:00
abstract：:The purpose of this study was to determine the contribution of long-chain fatty acids relative to other mitochondrial substrates in active vascular smooth muscle (VSM). Hog carotid arteries were isometrically contracted in physiological saline solution containing 0.71 mM U-(13)C-oleic acid (bound to albumin at a ratio...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000051056
更新日期：2001-05-01 00:00:00
abstract：:Neointima formation participates in the pathophysiology of atherosclerosis and restenosis. Proliferation and migration of vascular smooth muscle cells (VSMC) are initial responses to vascular injury. The aim of the present study was to assess the effect of gliotoxin, an inhibitor of nuclear factor (NF)-kappaB, on migr...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000176043
更新日期：2009-01-01 00:00:00
abstract：BACKGROUND/AIMS:Endoplasmic reticulum (ER) stress has emerged as a potential mechanism contributing to diabetes and its comorbidities. However, the importance of ER stress in diabetic vascular dysfunction is unclear. The purpose of this study was to examine the effects of the ER stress inhibitor, tauroursodeoxycholic a...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000479967
更新日期：2017-01-01 00:00:00
abstract：BACKGROUND:It has been reported that smoking is one of the strongest positive risk factors for abdominal aortic aneurysms (AAAs). Although many studies have been directed to decipher the effect of smoking on AAA, its effect on macrophage activation has not yet been explored. OBJECTIVES:We have reported the importance ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000498893
更新日期：2019-01-01 00:00:00
abstract：:In coronary artery diseases, cigarette smoking is a risk factor and the endothelin system plays a key role in the pathogenesis. This study was to examine if dimethylsulfoxide-soluble smoke particles (DSP) upregulate endothelin type-B (ETB) receptors in the coronary artery and investigate the mechanism. The isolated ra...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000350866
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:The aim of the present study was to evaluate different signaling pathways by which exercise training would interfere in endothelial function in obesity. Therefore, we examined adipocytokine levels and their receptors in the corpus cavernosum and femoral artery from trained rats on a high-fat diet. METHODS:F...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000457800
更新日期：2017-01-01 00:00:00
abstract：UNLABELLED:Nitric oxide (NO) and cyclooxygenase (COX) derived prostaglandins (PGs) are involved in vascular homeostasis. Contradictory results have been obtained in previous studies on the putative 'cross-talk' between these two pathways. Our aim was to evaluate the interaction between NO and PG release in human microv...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000074550
更新日期：2003-09-01 00:00:00
abstract：:Coronary bypass vessels, saphenous vein (SV) and internal thoracic artery (ITA), differ in susceptibility to atherosclerosis and medium- to long-term patency. Whereas most ITA remain patent (90% at 10 years), 20% of SV grafts fail in the first year and approximately 45% fail within 10 years. Reasons for these differen...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159245
更新日期：1997-09-01 00:00:00
abstract：:The effect of shear stress at the endothelium in the attenuation of the noradrenaline-induced constriction of the femoral vascular bed perfused at a constant blood flow was investigated in 16 anesthetized cats. It is known that the adrenergic vasoconstriction of the femoral vascular bed is considerably greater at a co...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000097750
更新日期：2007-01-01 00:00:00
abstract：:Peripheral vascular resistance is increased in essential hypertension. This involves structural changes of resistance arteries and stiffening of the arterial wall, including remodeling of the extracellular matrix. We hypothesized that biopsies of the human parietal pericardium, obtained during coronary artery bypass g...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000376548
更新日期：2015-01-01 00:00:00
abstract：:The pathophysiology of Raynaud's phenomenon (RP) remains an enigma. Whatever theories proposed, the final event leading to the clinical symptoms is the occlusion of digital vessels. However, the possibility that the upstream large arterial vessels contribute to vasopasm has never been investigated. We used a high reso...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159237
更新日期：1997-07-01 00:00:00
abstract：:Mesenteric endothelial cell function and immunoreactivity for substance P and nitric oxide synthase (NOS) were examined in control rats and rats treated with capsaicin as neonates to destroy primary afferent nerves. Endothelial vasodilator function was examined pharmacologically in the methoxamine raised-tone isolated...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159106
更新日期：1995-09-01 00:00:00
abstract：BACKGROUND:Low birth weight (LBW) is associated with increased risk of type 2 diabetes and cardiovascular disease. We studied endothelial function and insulin sensitivity in young men with LBW (n = 22) and controls (n = 22). METHODS:Insulin sensitivity and endothelial function was studied with venous occlusion plethys...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000235968
更新日期：2010-01-01 00:00:00
abstract：:Cx40-deficient mice (Cx40-/-) are hypertensive due to increased renin secretion. We evaluated the renal expression of neuronal nitric oxide synthase (nNOS) and cyclooxygenases COX-1 and COX-2, three macula densa enzymes. The levels of nNOS were increased in kidneys of Cx40-/- mice, as well as in those of wild-type (WT...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000156704
更新日期：2009-01-01 00:00:00
abstract：:Fibroblast growth factor-2 (FGF2) and vascular endothelial growth factor (VEGF) exert their angiogenic activity by interacting with endothelial cells in a distinct manner. In this study, we investigated the morphological features of endothelial cells of the chick embryo chorioallantoic membrane (CAM) microvasculature ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000051070
更新日期：2001-07-01 00:00:00
abstract：:This study utilised catecholamine fluorescence histochemistry, multiple-labelling immunohistochemistry and retrograde axonal transport to determine the distribution and neuropeptide content of sympathetic neurons innervating veins in the dorsal skin of the guinea pig pinna. There was a dense plexus of sympathetic axon...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159113
更新日期：1995-11-01 00:00:00
abstract：:The present study investigated transcellular signalling mechanism involved in thrombin-induced production of plasminogen activator inhibitor-1 (PAI-1) in cultured vascular baboon aortic smooth muscle cells (BASMC). Treatments with thrombin dose-dependently increased the steady state levels of PAI-1 mRNA and the genera...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159205
更新日期：1997-03-01 00:00:00
abstract：:This study was designed to establish the in vitro pharmacological responses of rat middle cerebral arteries (MCA), and also their susceptibility to overnight cold storage at 4 degrees C. MCAs were harvested from rats and pharmacological responses were studied using a Mulvany myograph. Responses to prostaglandin F2 alp...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159097
更新日期：1995-07-01 00:00:00
abstract：:This work compares the expression of adrenoceptors (ARs) and G-protein-coupled receptor kinase (GRK) 2 (RT-PCR and immunoblotting) and functional responses in conductance (aorta) and resistance vessels (mesenteric resistance arteries; MRA) in two different models of rat hypertension: hypertension induced by chronic tr...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000360400
更新日期：2014-01-01 00:00:00
abstract：:During the early stage of angiogenesis, neovascular sprouts are composed primarily of endothelial cells. As they mature, microvessels acquire a coating of mural cells, which are critical for the development and maintenance of a functional vasculature. Though growth factor regulation of mural cell recruitment has been ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000070711
更新日期：2003-03-01 00:00:00
abstract：:The localization of dopamine receptor subtypes in the rabbit pulmonary circulation was investigated by using combined radioligand binding and light microscope autoradiography techniques. Dopamine D1-like receptors, probably belonging to the dopamine D1 receptor subtype, were demonstrated in sections of rabbit lung usi...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159094
更新日期：1995-05-01 00:00:00
abstract：:The proinflammatory cytokine interleukin (IL)-1beta and the IL-1 receptor antagonist are expressed by atherosclerotic plaques and may be linked to the development of atherosclerosis. Existing evidence shows that retinoids and their receptors are involved in inflammatory response and that they are found in atherosclero...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000094258
更新日期：2006-01-01 00:00:00
abstract：:The D allele of the insertion (I)/deletion (D) polymorphism in the angiotensin-converting enzyme (ACE) gene and the C allele of the A1166-C polymorphism in the angiotensin II type 1 receptor (AGT1R) gene have been associated with altered vascular structure and with an increased risk of myocardial infarction. The aim o...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025687
更新日期：1999-11-01 00:00:00
abstract：:We examined the growth of the right common carotid artery of young rabbits after ligating the left common carotid artery at 3 weeks of age, a procedure that approximately doubled right carotid blood flows. Flow increased from 0.065 +/-0.003 to 0.096+/-0.009 ml/s within 1 h and, at 15 weeks of age, carotid blood flows ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025559
更新日期：1998-01-01 00:00:00
abstract：BACKGROUND:We have developed an in vitro model by organ culture of rat mesenteric arteries to imitate vascular smooth muscle cell (VSMC) receptor changes in cardiovascular disease. By using this model, alteration of VSMC thromboxane A(2) (TP) receptors was studied. METHODS AND RESULTS:After organ culture of the arteri...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000153247
更新日期：2009-01-01 00:00:00
abstract：:The effects of the close arterial infusion of histamine upon the microcirculation of facial and nasal tissues were examined in dogs. Blood flow through arteriovenous anastomoses (AVA flow), capillaries (CAP flow) and collaterals (COL flow) were determined by electromagnetic flowmetry and the tracer-microsphere techniq...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000158972
更新日期：1993-01-01 00:00:00
