Oleate oxidation and mitochondrial substrate selection in vascular smooth muscle.

Abstract:

:The purpose of this study was to determine the contribution of long-chain fatty acids relative to other mitochondrial substrates in active vascular smooth muscle (VSM). Hog carotid arteries were isometrically contracted in physiological saline solution containing 0.71 mM U-(13)C-oleic acid (bound to albumin at a ratio of 6.8:1), 5 mM 1-(13)C-glucose and 1 mM acetate in the presence or absence of 5 mM carnitine for 6 h at 37 degrees C. Substrate oxidation was determined using (13)C-isotopomer analysis of glutamate. Although oxidation of oleic acid could not be measured at physiological concentrations [0.5 mM (1:1)], oleic acid oxidation was approximately 5% of the total substrates oxidized at the higher concentration examined. Although insignificant, carnitine increased oleic acid oxidation to approximately 8%, and resulted in a decrease in endogenous lipid oxidation, which was 2-12% of the total substrates oxidized. Oxidation of glucose and acetate did not significantly change due to the inclusion of oleic acid in the incubation solutions. Therefore, we conclude that exogenous long-chain fatty acids are minor contributors to substrate oxidation (approximately 5%) in VSM compared to other mitochondrial substrates, such as glucose and acetate, which account for approximately 80% of the substrates oxidized by VSM.

journal_name

J Vasc Res

authors

Allen TJ,Hardin CD

doi

10.1159/000051056

keywords:

subject

Has Abstract

pub_date

2001-05-01 00:00:00

pages

276-87

issue

3

eissn

1018-1172

issn

1423-0135

pii

51056

journal_volume

38

pub_type

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