Abstract：
:This work compares the expression of adrenoceptors (ARs) and G-protein-coupled receptor kinase (GRK) 2 (RT-PCR and immunoblotting) and functional responses in conductance (aorta) and resistance vessels (mesenteric resistance arteries; MRA) in two different models of rat hypertension: hypertension induced by chronic treatment with L-NAME (N(G)-nitro-L-arginine methyl-ester) (L-NAME-treated rats; LNHR), and genetically induced hypertension (spontaneously hypertensive rats; SHR). Changes found in the aorta, but not in the MRA, were: (1) a loss of contractile capacity, more evidently in α1-AR-mediated contraction, and an impairment of endothelium-dependent vasorelaxation, with both changes occurring independently of the hypertensive model; (2) a diminished sensitivity to α1-AR-induced vasoconstriction along with increased β2-AR-mediated vasodilation in LNHR, and (3) a lower expression of ARs and GRK2 in LNHR. The two latter changes are the opposite of those previously found in aortas of SHR. In the MRA of LNHR, a diminished sensitivity to isoprenaline, in parallel with a reduced expression of β1-AR, was observed without changes in GRK2 expression. In the MRA of SHR, the increased GRK2 expression was not accompanied by significant changes in either β-AR expression or the vasorelaxant potency of isoprenaline. The present results highlight that changes in AR function differ not only between vessels but also between hypertensive models. Moreover, they suggest that changes in GRK2 expression could contribute to regulating β2-AR function in conductance vessels but not β1-AR function in resistance vessels.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Oliver E,Flacco N,Arce C,Ivorra MD,D'Ocon MP,Noguera MAdoi
10.1159/000360400subject
Has Abstractpub_date
2014-01-01 00:00:00pages
209-20issue
3eissn
1018-1172issn
1423-0135pii
000360400journal_volume
51pub_type
杂志文章abstract：BACKGROUND:We have developed an in vitro model by organ culture of rat mesenteric arteries to imitate vascular smooth muscle cell (VSMC) receptor changes in cardiovascular disease. By using this model, alteration of VSMC thromboxane A(2) (TP) receptors was studied. METHODS AND RESULTS:After organ culture of the arteri...
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journal_title：Journal of vascular research
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journal_title：Journal of vascular research
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doi：10.1159/000063687
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更新日期：1999-01-01 00:00:00
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abstract：:The localization of dopamine receptor subtypes in the rabbit pulmonary circulation was investigated by using combined radioligand binding and light microscope autoradiography techniques. Dopamine D1-like receptors, probably belonging to the dopamine D1 receptor subtype, were demonstrated in sections of rabbit lung usi...
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