Abstract:
:During the early stage of angiogenesis, neovascular sprouts are composed primarily of endothelial cells. As they mature, microvessels acquire a coating of mural cells, which are critical for the development and maintenance of a functional vasculature. Though growth factor regulation of mural cell recruitment has been extensively investigated, the intracellular signaling events involved in this process remain poorly understood. Among the intracellular kinases implicated in angiogenesis, the p38 MAPK has been shown to transduce signals critical for vascular remodeling and maturation. The rat aorta model of angiogenesis was used to further investigate the role of this signaling pathway in the recruitment of mural cells during angiogenesis. The p38 MAPK inhibitor SB203580 selectively blocked mural cell recruitment, resulting in the formation of naked endothelial tubes without mural cells. SB203580 inhibited angiopoietin-1-induced mural cell recruitment without influencing angiopoietin-1-stimulated endothelial sprouting. Adenoviral vector-mediated expression of a dominant negative form of p38 MAPK significantly reduced mural cell recruitment, whereas overexpression of a constitutively activated form of MKK6, an upstream activator of p38 MAPK, increased mural cell number. These results indicate that the p38 MAPK signaling pathway plays a critical role in mural cell recruitment during neovascularization and may represent a therapeutic target in angiogenesis-related disorders.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Zhu WH,Han J,Nicosia RFdoi
10.1159/000070711keywords:
subject
Has Abstractpub_date
2003-03-01 00:00:00pages
140-8issue
2eissn
1018-1172issn
1423-0135pii
70711journal_volume
40pub_type
杂志文章abstract::Cx40-deficient mice (Cx40-/-) are hypertensive due to increased renin secretion. We evaluated the renal expression of neuronal nitric oxide synthase (nNOS) and cyclooxygenases COX-1 and COX-2, three macula densa enzymes. The levels of nNOS were increased in kidneys of Cx40-/- mice, as well as in those of wild-type (WT...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000156704
更新日期:2009-01-01 00:00:00
abstract::Coronary bypass vessels, saphenous vein (SV) and internal thoracic artery (ITA), differ in susceptibility to atherosclerosis and medium- to long-term patency. Whereas most ITA remain patent (90% at 10 years), 20% of SV grafts fail in the first year and approximately 45% fail within 10 years. Reasons for these differen...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159245
更新日期:1997-09-01 00:00:00
abstract::Tumor necrosis factor (TNF) receptor-associated factors (TRAFs) are cytoplasmic adaptor proteins of the TNF/interleukin (IL)-1/Toll-like receptor superfamily. Ligands of this family such as TNFα, CD40L, and IL-1β promote chronic inflammatory processes such as atherosclerosis and restenosis, the latter being a common a...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000501615
更新日期:2019-01-01 00:00:00
abstract::In this study we aimed to investigate whether the therapeutic efficacy of anisodamine in the treatment of bacteraemic shock could--at least in part--be brought about by its direct interference with the lipopolysaccharide (LPS)-induced activation of endothelial cells. Thus, we investigated the effect of anisodamine on ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000051025
更新日期:2001-01-01 00:00:00
abstract:PURPOSE:Exercise elicits early adaptation of coronary vessels enabling the coronary circulation to respond adequately to higher flow demands. We hypothesized that short-term daily exercise induces biomechanical and functional remodeling of the coronary resistance arteries related to pressure. METHODS:Male rats were su...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000486571
更新日期:2018-01-01 00:00:00
abstract::This study was designed to establish the in vitro pharmacological responses of rat middle cerebral arteries (MCA), and also their susceptibility to overnight cold storage at 4 degrees C. MCAs were harvested from rats and pharmacological responses were studied using a Mulvany myograph. Responses to prostaglandin F2 alp...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159097
更新日期:1995-07-01 00:00:00
abstract::The pH has marked effects on the blood flow in several vascular beds but the underlying mechanisms remain incompletely understood. It is still not agreed, for example, whether it is the fall in extracellular pH or intracellular pH that is responsible for changes in tone resulting from hypercapnic acidosis. The issue h...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000159163
更新日期:1996-09-01 00:00:00
abstract:BACKGROUND/AIMS:Endothelial nitric oxide synthase (eNOS) is associated with caveolin-1 (Cav-1) in plasma membrane. We tested the hypothesis that eNOS activation by shear stress in resistance vessels depends on synchronized phosphorylation, dissociation from Cav-1 and translocation of the membrane-bound enzyme to Golgi ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000355301
更新日期:2013-01-01 00:00:00
abstract::Organic nitrates act as vasodilators and have long been used for treatment of cardiovascular diseases. However, the therapeutic effect of nitrates is limited by induction of nitrate tolerance which is associated with endothelial dysfunction and enhanced vasoconstriction. Multiple mechanisms cause nitrate tolerance inc...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000065542
更新日期:2002-07-01 00:00:00
abstract::Dynamic visualization of the intravascular events leading to the extravasation of leukocytes into tissues by intravital microscopy has significantly expanded our understanding of the underlying molecular processes. In contrast, the detailed observation of leukocyte transendothelial and interstitial migration in vivo h...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000073902
更新日期:2003-09-01 00:00:00
abstract::Previous studies demonstrated that maintenance of steady-state myogenic tone requires Ca(2+)-dependent myosin phosphorylation. The present studies furthered these observations by examining temporal relationships among Ca(2+), myosin phosphorylation and vessel diameter during acute increases in intraluminal pressure an...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000054089
更新日期:2000-11-01 00:00:00
abstract::Signaling pathways activated by the tachykinin substance P (SP) were investigated in pig coronary artery endothelial cells (PCAECs). Single cells were obtained after enzymatic digestion of coronary arteries. Intracellular Ca2+ ([Ca2+]i) was measured from fura-2 fluorescence while membrane potential or ionic current wa...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159222
更新日期:1997-05-01 00:00:00
abstract:BACKGROUND AND AIMS:Vasomotion consists in cyclic oscillations of the arterial diameter induced by the synchronized activity of the smooth muscle cells. So far, contradictory results have emerged in the literature about the role of the endothelium in the onset and maintenance of vasomotion. Here our aim is to understan...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000124283
更新日期:2008-01-01 00:00:00
abstract::We examined the growth of the right common carotid artery of young rabbits after ligating the left common carotid artery at 3 weeks of age, a procedure that approximately doubled right carotid blood flows. Flow increased from 0.065 +/-0.003 to 0.096+/-0.009 ml/s within 1 h and, at 15 weeks of age, carotid blood flows ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025559
更新日期:1998-01-01 00:00:00
abstract:AIMS:Percutaneous coronary intervention is routinely performed to treat occlusive coronary artery disease. Coronary perforation is a potential complication and can be treated with a stent graft. Current stent grafts are associated with high restenosis rates. We tested the safety and feasibility of biodegradable stent g...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000505454
更新日期:2020-01-01 00:00:00
abstract::Intima-media thickening (IMT) of the carotid artery, a form of vascular remodeling, correlates well with coronary artery disease risk in humans. Vascular remodeling in response to blood flow is a complex process that critically involves altered cell matrix interactions. To gain insight into these events, we performed ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000081804
更新日期:2004-11-01 00:00:00
abstract::Most virus-infected cells release interferon-beta (IFN-beta) as a powerful inducer of antiviral defense. Endothelial cells tightly regulate local immune cell recruitment by expression of adhesion molecules and chemokines. Here, we studied the transcriptional regulation of IFN-beta-induced chemokine expression in prima...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000097977
更新日期:2007-01-01 00:00:00
abstract::Chemokines play an important role in the process of leukocyte recruitment and activation at sites of inflammation. Until recently, the actions of chemokines and the expression of their receptors have only been described on different leukocyte populations. However, increasing evidence has suggested that non-haematopoie...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000025707
更新日期:2000-01-01 00:00:00
abstract::The influence of vascular endothelium on ouabain-induced contractions and sodium pump activity in aortic segments of Wistar-Kyoto (WKY) and spontaneously hypertensive rat (SHR) was analyzed. De-endothelialization increased and reduced ouabain-induced contractions in WKY and SHR segments, respectively. The effects of d...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159145
更新日期:1996-03-01 00:00:00
abstract::Protein localization in endothelial cells is tightly regulated to create distinct signaling domains within their tight spatial restrictions including luminal membranes, abluminal membranes, and interendothelial junctions, as well as caveolae and calcium signaling domains. Protein localization in endothelial cells is a...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000512618
更新日期:2021-01-27 00:00:00
abstract::In response to stimuli, endothelial cells release arachidonic acid, a lipid precursor of various vasoactive substances. We have investigated the relationships between cytosolic Ca2+ movements and arachidonic acid release in human umbilical vein endothelial cells. Histamine, a receptor-dependent agonist, and thapsigarg...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025647
更新日期:1999-05-01 00:00:00
abstract::Increased vascular disease occurs with low albumin (human serum albumin, HSA), possibly reflecting specific inhibition of endothelial apoptosis reported for tissue culture. Despite the reported specificity for endothelial protection by HSA, the high but physiological concentrations needed appear more consistent with n...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000101777
更新日期:2007-01-01 00:00:00
abstract:OBJECTIVE:It is well known that inflammation plays key roles in the development of atherosclerosis and that the transplantation of bone marrow mononuclear cells (BMMNCs) can suppress inflammation in rodent models of ischemic diseases. Here, we explored whether transplantation of autologous BMMNCs could prevent the prog...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000449201
更新日期:2016-01-01 00:00:00
abstract:AIM:Vascular remodeling following injury substantially accounts for restenosis and adverse clinical outcomes. In this study, we investigated the role of the giant scaffold protein Ahnak1 in vascular healing after endothelial denudation of the murine femoral artery. METHODS:The spatiotemporal expression pattern of Ahna...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000464287
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVES:Extracellular nucleotide release at the site of arterial injury mediates the proliferation and migration of vascular smooth muscle cells. Our aim was to investigate the role of the P2Y2 nucleotide receptor (P2Y2R) in neointimal hyperplasia. Approach and Results: Vascular injury was induced by the implantatio...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000449059
更新日期:2016-01-01 00:00:00
abstract::Migration of vascular smooth muscle cells (SMCs) leading to neointimal hyperplasia is an early and cardinal feature of atherogenesis. Migration of rat aortic SMCs from an upper chamber towards a lower one has been studied in a microchemotaxis (Boyden) chamber. Spontaneous migration of SMCs was practically prevented by...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025580
更新日期:1998-05-01 00:00:00
abstract::The Ig-like cell adhesion molecule ICAM-3 is mainly expressed on human leukocytes and is involved in cell-cell interactions. Its expression on endothelium is observed during disorders such as Crohn's disease and in solid tumors. We found low but detectable expression of ICAM-3 on VE-cadherin-expressing cells from prim...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000076126
更新日期:2004-01-01 00:00:00
abstract::Dietary flavonoids are thought to protect against cardiovascular disease. We have studied the effects of bioactive isoflavone metabolites on hyperlipidemia, endothelial dysfunction and the development of atherosclerotic lesions in apolipoprotein E-deficient (apoE(0)) mice fed a Western high-fat diet. Supplementation w...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000071891
更新日期:2003-05-01 00:00:00
abstract::The circadian clock is rhythmically expressed in blood vessels, but the interaction between the circadian clock and disturbed blood flow remains unclear. We examined the relationships between BMAL1 and CLOCK and 2 regulators of endothelial function, AKT1 and endothelial nitric oxide synthase (eNOS), in vascular region...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000452410
更新日期:2016-01-01 00:00:00
abstract::The present study investigated transcellular signalling mechanism involved in thrombin-induced production of plasminogen activator inhibitor-1 (PAI-1) in cultured vascular baboon aortic smooth muscle cells (BASMC). Treatments with thrombin dose-dependently increased the steady state levels of PAI-1 mRNA and the genera...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159205
更新日期:1997-03-01 00:00:00